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Volumn 106, Issue 38, 2002, Pages 9847-9852

Temperature dependence of the biotin-avidin bond-rupture force studied by atomic force microscopy

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN-AVIDIN BOND-RUPTURE FORCE;

EID: 0037180006     PISSN: 10895647     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp020863+     Document Type: Article
Times cited : (47)

References (35)
  • 11
    • 0018101150 scopus 로고
    • Bell, G.I. Science 1978, 200, 618-627.
    • (1978) Science , vol.200 , pp. 618-627
    • Bell, G.I.1
  • 12
    • 0011778841 scopus 로고    scopus 로고
    • note
    • 18.
  • 23
    • 0011772639 scopus 로고    scopus 로고
    • note
    • -4 s, depending on the rate of force loading applied.
  • 25
    • 0011717101 scopus 로고    scopus 로고
    • note
    • 24 Avidin, an analogue to streptavidin, shares 38% sequence identity and is assumed to exhibit similar bonding with biotin.
  • 28
    • 0011747324 scopus 로고    scopus 로고
    • note
    • 7,10,30 MD is still currently limited to events of subpicosecond time duration, making its direct relevance to millisecond experiments such as ours questionable. Even with a significant enhancement in computer speed, it will remain a great challenge to simulate AFM bond-rupture processes occurring on the millisecond time scale with MD. Thus we chose to apply a thermodynamic approach to interpret the temperature dependence of unbinding forces in this study.
  • 29
    • 0011717562 scopus 로고    scopus 로고
    • note
    • 18 if the last remaining set of linkages were not of equal strength, or were not loaded similarly, this would manifest in the variance vs mean plot (e.g., see Figure 2B) as curvature. This did not occur in our systems, which further supports this assumption.
  • 31
    • 0011745061 scopus 로고    scopus 로고
    • note
    • e into bond stretching energy to effect rupture. Of course, the actual potential is neither of the Morse nor 6-12 form precisely. Nevertheless, these examples illustrate that probably only 20-25% of the total bond dissociation energy must be deposited before the critical distance is reached.
  • 33
    • 0011715885 scopus 로고    scopus 로고
    • note
    • -1), consistent with our findings.
  • 34
    • 0011717563 scopus 로고    scopus 로고
    • note
    • -1) is reasonable for the biotin-avidin complex, a strong noncovalent binding that contains several hydrogen bonds and van der Waals interactions.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.