Loss of μ-opioid receptor-mediated G-protein activation in the pons/medulla of mice lacking the exons 2 and 3 of μ-opioid receptor gene

Neuroscience Letters

Volumn 335, Issue 2, 2002, Pages 91-94

  Mizoguchi, Hirokazu ^a   Wu, Hsiang En ^a   Narita, Minoru ^b   Loh, Horace H ^c   Nagase, Hiroshi ^d   Tseng, Leon F ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b HOSHI UNIVERSITY   (Japan)

^c UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^d TORAY INDUSTRIES INC   (Japan)

Author keywords

opioid receptor; opioid receptor; G protein activation; Mouse; Pons medulla

Indexed keywords

ANISOLE; BETA ENDORPHIN; DEXTRO ALANINE; ENDOMORPHIN 1; ENDOMORPHIN 2; ENKEPHALIN DERIVATIVE; GLYCOL ENKEPHALIN; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); MU OPIATE RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BRAIN MEMBRANE; CONTROLLED STUDY; ENZYME BINDING; EXON; EXON 2; EXON 3; GENE SEQUENCE; GENETIC CODE; KNOCKOUT MOUSE; MEDULLA OBLONGATA; MOUSE; NONHUMAN; PONS; PRIORITY JOURNAL; PROTEIN TARGETING; RECEPTOR GENE;

ANALGESICS, OPIOID; ANALYSIS OF VARIANCE; ANIMALS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENKEPHALIN, ALA(2)-MEPHE(4)-GLY(5)-; EXONS; GENE DELETION; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); MEDULLA OBLONGATA; MEMBRANES; MICE; MICE, KNOCKOUT; OLIGOPEPTIDES; RECEPTORS, OPIOID, MU; SULFUR ISOTOPES;

EID: 0037176186     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(02)01171-0     Document Type: Article

References (18)

1. Chang J.K., Chang K.-J. The role of amino-terminal sequence of β-endorphin and dynorphin in the determination of opioid receptor type selectivity. Life Sci. 33(Suppl. 1):1983;267-269.
2. Chen Y., Mestek A., Liu J., Hurley A., Yu L. Molecular cloning and functional expression of a μ-opioid receptor from rat brain. Mol. Pharmacol. 44:1993;8-12.
3. Loh H.H., Liu H.C., Cavalli A., Yang W.L., Chen Y.F., Wei L.N. μ opioid receptor knockout in mice: effects on ligand-induced analgesia and morphine lethality. Mol. Brain Res. 54:1998;321-326.
4. Matthes H.W.D., Maldonado R., Simonin F., Valverde O., Slowe S., Kitchen I., Befort K., Dierich A., Le M.M., Dolle P., Tzavara E., Hanoune J., Roques B.P., Kieffer B.L. Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the μ-opioid-receptor gene. Nature. 383:1996;819-823.
5. Mansour A., Khachaturian H., Lewis M.E., Akil H., Watson S.J. Anatomy of CNS opioid receptors. Trends Neurosci. 11:1988;308-314.
6. Mizoguchi H., Narita M., Nagase H., Tseng L.F. Activation of G-proteins in the mouse pons/medulla by β-endorphin is mediated by the stimulation of μ- and ε-receptors. Life Sci. 67:2000;2733-2743.
7. Mizoguchi H., Narita M., Oji D.E., Suganuma C., Nagase H., Sora I., Uhl G.R., Cheng E.Y., Tseng L.F. The μ-opioid receptor gene-dose dependent reductions in G-protein activation in the pons/medulla and antinociception induced by endomorphins in μ-opioid receptor knockout mice. Neuroscience. 94:1999;203-207.
8. 1-opioid receptors in endomorphin- and β-endorphin-induced G-protein activation in the mouse pons/medulla. Neuroscience. 100:2000;835-839.
9. Mizoguchi H., Wu H.-E., Narita M., Hall F.S., Sora I., Uhl G.R., Nagase H., Tseng L.F. Antagonistic property of buprenorphine for putative ε-opioid receptor-mediated G-protein activation by β-endorphin in pons/medulla of the μ-opioid receptor knockout mouse. Neuroscience. 2002;. (in press).
10. Narita M., Mizoguchi H., Narita M., Sora I., Uhl G.R., Tseng L.F. Absence of G-protein activation by μ-opioid receptor agonists in the spinal cord of μ-opioid receptor knockout mice. Br. J. Pharmacol. 126:1999;451-456.
11. 35S]GTPγS binding in the mouse spinal cord. Eur. J. Pharmacol. 351:1998;383-387.
12. Nock B., Giordano A.L., Cicero T.J., O'Connor L.H. Affinity of drugs and peptides for U-69,593-sensitive and -insensitive κ opiate binding sites: the U-69,593-insensitive site appears to be the β-endorphin-specific ε receptor. J. Pharmacol. Exp. Ther. 254:1990;412-419.
13. Nock B., Giordano A.L., Moore B.W., Cicero T.J. Properties of the putative ε opioid receptor: identification in rat, guinea pig, cow, pig and chicken brain. J. Pharmacol. Exp. Ther. 264:1993;349-359.
14. Raynor K., Kong H., Chen Y., Yasuda K., Yu L., Bell G.I., Reisine T. Pharmacological characterization of the cloned κ-, δ-, and μ-opioid receptors. Mol. Pharmacol. 45:1994;330-334.
15. Schuller A.G., King M.A., Zhang J., Bolan E., Pan Y.X., Morgan D.J., Chang A., Czick M.E., Unterwald E.M., Pasternak G.W., Pintar J.E. Retention of heroin and morphine-6β-glucuronide analgesia in a new line of mice lacking exon 1 of MOR-1. Nat. Neurosci. 2:1999;151-156.
16. Sora I., Takahashi N., Funada M., Ujike H., Revay R.S., Donovan D.M., Miner L.L., Uhl G.R. Opiate receptor knockout mice define μ receptor roles in endogenous nociceptive responses and morphine-induced analgesia. Proc. Natl. Acad. Sci. USA. 94:1997;1544-1549.
17. Tseng L.F. Evidence for ε-opioid receptor-mediated β-endorphin-induced analgesia. Trends Pharmacol. Sci. 22:2001;623-630.
18. 35S]thio)triphosphate binding to membranes from human neuroblastoma SH-SY5Y cells. Mol. Pharmacol. 47:1995;848-854.