Absence of G-protein activation by μ-opioid receptor agonists in the spinal cord of μ-opioid receptor knockout mice

British Journal of Pharmacology

Volumn 126, Issue 2, 1999, Pages 451-456

  Narita, Minoru ^a   Mizoguchi, Hirokazu ^a   Narita, Michiko ^a   Sora, Ichiro ^b   Uhl, George R ^b   Tseng, Leon F ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b NATIONAL INSTITUTE ON DRUG ABUSE   (United States)

Author keywords

Endomorphins; G proteins; Homologous recombination; Knockout mice; Opioid peptides; Signal transduction; Spinal cord; opioid receptors

Indexed keywords

3,4 DICHLORO N METHYL N [2 (1 PYRROLIDINYL)CYCLOHEXYL]BENZENEACETAMIDE METHANESULFONATE; ENDOMORPHIN 1; ENDOMORPHIN 2; ENKEPHALIN[2 DEXTRO ALANINE 4 METHYLPHENYLALANINE 5 GLYCINE]; ENKEPHALIN[2,5 DEXTRO PENICILLAMINE]; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); MU OPIATE RECEPTOR; MU OPIATE RECEPTOR AGONIST;

ANIMAL TISSUE; ARTICLE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SCATCHARD PLOT; SPINAL CORD;

EID: 0032908151     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0702330     Document Type: Article

References (21)

1. 35S]thio)triphosphate binding by endogenous opioids acting at a cloned mu receptor. J. Pharmacol. Exp. Ther., 286, 282-288.
2. Chavkin, C. & Goldstein, A. (1984). Opioid receptor reserve in normal and morphine-tolerant guinea pig ileum myenteric plexus. Proc. Natl. Acad. Sci. U.S.A., 81, 7253-7257.
3. Chen, Y., Mestek, A., Liu, J., Hurley, J.A. & Yu, L. (1993). Molecular cloning and functional expression of a μ-opioid receptor from the rat brain. Mol. Pharmacol., 44, 8-12.
4. Harrison, L.M., Kastin, A.J. & Zadina, J.E. (1998). Differential effects of endomorphin-1, endomorphin-2, and Tyr-W-MIF-1 on activation of G-proteins in SH-SY5Y human neuroblastoma membranes. Peptides, 19, 749-753.
5. Kitchen, I., Slowe, S.J., Matthes, H.W.-D. & Kieffer, B. (1997). Quantitative autoradiographic mapping of μ-, δ-and κ-opioid receptors in knockout mice lacking the μ-opioid receptor gene. Brain Res., 778, 73-88.
6. Law, P.-Y. (1995). G-proteins and opioid receptors' function. In The Pharmacology of Opioid Peptides, ed. Tseng, L.F. Amsterdam: Harwood Academic Publishers, pp.109-130.
7. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
8. Matthes, H.W.D., Maldonado, R., Simonin, F., Valverde, O., Slowe, S., Kitchen, I., Befort, K., Dierich, A., Le, M.M., Dolle, P., Tzavara, E., Hanoune, J., Roques, B.P. & Kieffer, B.L. (1996). Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the mu-opioid-receptor gene. Nature, 383, 819-823.
9. Matthes, H.W.D., Smadja, C., Valverde, O., Vonesch, J.-L., Foutz, A.S., Boudinot, E., Denavit-Saubié, M., Severini, C., Negri, L., Roques, B.P, Maldonado, R. & Kieffer, B.L. (1998). Activity of the δ-opioid receptor is partially reduced, whereas activity of the κ-receptor is maintained in mice lacking the μ-receptor. J. Neuroscience, 18, 7285-7295.
10. 35S]GTPγS binding in the mouse spinal cord. Eur. J. Pharmacol., 351, 383-387.
11. Narita, M. & Tseng, L.F. (1998). Evidence for the existence of the β-endorphin-sensitive 'ε-opioid receptor' in the brain: the mechanisms of ε-mediated antinociception. Jpn. J. Pharmacol., 76, 233-253.
12. Selly, D.E., Sim, L.J., Xiao, R., Liu, Qixu & Childers, S.R (1997). μ-Opioid receptor-stimulated guanosin-5-O-(γ-thio)-triphosphate binding in rat thalamua and cultured cell lines: Signal transduction mechanisms underlying agonist efficacy. Mol. Pharmacol., 51, 87-96.
13. 35S]GTPγS binding in rat brain: Evidence for partial agonist activity at μ-opioid receptors. J. Neurochem., 70, 1567-1576.
14. 35S]thio]-triphosphatc binding. Proc. Natl. Acad. Sci. USA, 92, 7242-7246.
15. Sim, J.S., Selly, D.E., Xiao, R. & Childers, S.R. (1996). Differences in G-protein activation by μ-and δ-opioid, and cannabinoid, receptors in rat striatum. Eur. J. Pharmacol., 307, 97-105.
16. 35S]GTPγS binding in comparison with opioid agonist-stimulated ones in brain regions of the mice. Neurosci. Lett., 237, 113-116.
17. 5]enkephalin-induced analgesia. Eur. J. Pharmacol., 324, R1-R2.
18. Sora, I., Takahashi, N., Funada, M., Ujike, H., Revay, R.S., Donovan, D.M., Miner, L.L. & Uhl, G.R. (1997b). Opiate receptor knockout mice define μ receptor roles in endogenous nociceptive responses and morphine-induced analgesia. Proc. Natl. Acad. Sci. U.S.A., 94, 1544-1549.
19. Tallarida, R.J. & Murray, R.B. (1987). Manual of Pharmacological Calculation with Computer Programs. New York: Springer.
20. 35S]thio)triphosphate binding to membranes from human neuroblastoma SH-SY5Y cells. Mol. Pharmacol., 47, 848-854.
21. Zadina, J., Hackler, L., Ge, L.-J. & Kastin, A.J. (1997). A potent and selective endogenous agonist for the μ-opiate receptor. Nature, 386, 499-502.