Anchorage of cyclodextrin glucanotransferase on the outer membrane of Escherichia coli

Biotechnology and Bioengineering

Volumn 79, Issue 4, 2002, Pages 457-464

  Wan, Hsiao Ming ^a   Chang, Ban Yang ^a   Lin, Sung Chyr ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Cyclodextrin glucanotransferase; E. coli; Surface display

Indexed keywords

CELL MEMBRANES; CLONING; ENZYMES; ESCHERICHIA COLI; GENES;

CELL DEBRIS FRACTIONS;

BIOCHEMICAL ENGINEERING;

CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; HYBRID PROTEIN; PROTEINASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL FRACTIONATION; ENZYME ACTIVITY; ENZYME CONFORMATION; ESCHERICHIA COLI; IMMUNOBLOTTING; OUTER MEMBRANE; POLYMERASE CHAIN REACTION;

BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GLUCOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 0037143790     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10301     Document Type: Article

References (32)

1. Expressing genes in different Escherichia coli compartments
2. Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface
3. Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies
4. Flow cytometric screening of cell-based libraries
5. Transport and anchoring of μ-lactamase to the external surface of Escherichia coli
6. Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface
7. Specific adhesion and hydrolysis of cellulose by intact Escerichia coli expressing surface anchored cellulase or cellulose binding domains
8. Analysis of mutations in cyclodextrin glucanotransferase from Bacillus stearothermophilus which affect cyclization characteristics and thermostability
9. Practical applications of engineering Gram-negative bacterial cell surfaces
10. Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines
11. Immobilization of starch-degrading enzymes. I. A comparative study on soluble and immobilized cyclodextrin glucanotransferase
12. Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae
13. Immobilized cyclomaltodextrin glucanotransferase of an alkalophilic Bacillus sp. No. 38-32
14. Cell surface display of hepatitis B virus surface antigen by using Pseudomonas syringae ice nucleation protein
15. Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variants
16. Extracellular transport of cholera toxin B subunit using Neisseria IgA protease b-domian: Conformation-dependent outer membrane translocation
17. Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptide
18. Formation of bacterial membrane ice-nucleating lipoglycoprotein complexes
19. Export of an N-terminal fragment of Escherichia coli flagellin by a flagellum-specific pathway
20. Selection of casmium specific hexapeptides and their expression as OmpA fusion proteins in Escherichia coli
21. Purification and properties of cyclodextrin glucanotransferase of an alkalophilic Bacillus sp
22. Production of Schardinger β-dextrin by soluble and immobilized cyclodextrin glucanotransferase of an alkalophilic Bacillus sp
23. Secretion of the cell surface lipoprotein pullulanse by Escherichia coli: Collaboration or competition between the specific secretion pathway and the lipoprotein sorting pathway
24. Display of green fluorescent protein on Escherichia coli cell surface
25. Cell surface display of organophosphorus hydrolase using ice nucleation protein
26. Enhanced metalloadsorption of bacteria cells displaying poly-His peptides
27. Bacterial surface display: Trends and progress
28. Characterization of Escherichia coli expressing an Lpp'OmpA(46-159)-PhoA fusion protein localized in the outer membrane
29. Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae: Effects of insert position and host background
30. The cyclodextrins and their application sin biotechnology
31. Bacterial cyclodextrin glucanotranferase