Identification of a novel microtubule-associated protein that regulates microtubule organization and cytokinesis by using a GFP-screening strategy

Current Biology

Volumn 12, Issue 22, 2002, Pages 1946-1951

  Manabe, Ri Ichiroh ^a,b   Whitmore, Leanna ^a   Weiss, Jonathan M ^a   Horwitz, Alan Rick ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b AICHI MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSKELETON PROTEIN; GREEN FLUORESCENT PROTEIN; MICROTUBULE ASSOCIATED PROTEIN; COMPLEMENTARY DNA; GLFND PROTEIN, HUMAN; HYBRID PROTEIN; PHOTOPROTEIN; TUBULIN;

CELL DIVISION; CHO CELL; CYTOKINESIS; DNA SCREENING; GENETIC REGULATION; GENETIC SCREENING; MICROTUBULE ORGANIZING CENTER; OPITZ SYNDROME; PROTEIN ANALYSIS; ANIMAL; ARTICLE; CYTOLOGY; DNA MICROARRAY; FIBROBLAST; GENE DELETION; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METABOLISM; METHODOLOGY; MICROTUBULE; MOLECULAR GENETICS; PHYSIOLOGY; ULTRASTRUCTURE;

ANIMALS; CELL DIVISION; CHO CELLS; CRICETINAE; DNA, COMPLEMENTARY; FIBROBLASTS; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; TRANSFECTION; TUBULIN;

EID: 0037137436     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(02)01299-X     Document Type: Article

References (25)

1. Kirschner, M., and Mitchison, T. (1986). Beyond self-assembly: From microtubules to morphogenesis. Cell 45, 329-342.
2. Horwitz, A.R., and Parsons, J.T. (1999). Cell migration: Movin' on. Science 286, 1102-1103.
3. Rogers, S.L., and Gelfand, V.I. (2000). Membrane trafficking, organelle transport and the cytoskeleton. Curr. Opin. Cell Biol. 12, 57-62.
4. Cassimeris, L. (1999). Accessory protein regulation of microtubule dynamics throughout the cell cycle. Curr. Opin. Cell Biol. 11, 134-141.
5. Drewes, G., Ebneth, A., and Mandelkow, E.M. (1998). MAPs, MARKs and microtubule dynamics. Trends Biochem. Sci. 23, 307-311.
6. Quaderi, N.A., Schweiger, S., Gaudenz, K., Franco, B., Rugarli, E.I., Berger, W., Feldman, G.J., Volta, M., Andolfi, G., Gilgenkrantz, S., et al. (1997). Opitz G/BBB syndrome, a defect of midline development, is due to mutations in a new RING finger gene on Xp22. Nat. Genet. 17, 285-291.
7. Sawin, K.E., and Nurse, P. (1996). Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein. Proc. Natl. Acad. Sci. USA 93, 15146-15151.
8. Rolls, M.M., Stein, P.A., Taylor, S.S., Ha, E., McKeon, F., and Rapoport, T.A. (1999). A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. J. Cell Biol. 146, 29-44.
9. Fujii, G., Tsuchiya, R., Ezoe, E., and Hirohashi, S. (1999). Analysis of nuclear localization signals using a green fluorescent protein-fusion protein library. Exp. Cell Res. 251, 299-306.
10. Schweiger, S., Foerster, J., Lehmann, T., Suckow, V., Muller, Y.A., Walter, G., Davies, T., Porter, H., van Bokhoven, H., Lunt, P.W., et al. (1999). The Opitz syndrome gene product, MID1, associates with microtubules. Proc. Natl. Acad. Sci. USA 96, 2794-2799.
11. Perry, J., Short, K.M., Romer, J.T., Swift, S., Cox, T.C., and Ashworth, A. (1999). FXY2/MID2, a gene related to the X-linked Opitz syndrome gene FXY/MID1, maps to Xq22 and encodes a FNIII domain-containing protein that associates with microtubules. Genomics 62, 385-394.
12. Cox, T.C., Allen, L.R., Cox, L.L., Hopwood, B., Goodwin, B., Haan, E., and Suthers, G.K. (2000). New mutations in MID1 provide support for loss of function as the cause of X-linked Opitz syndrome. Hum. Mol. Genet. 9, 2553-2562.
13. Olson, K.R., McIntosh, J.R., and Olmsted, J.B. (1995). Analysis of MAP 4 function in living cells using green fluorescent protein chimeras. J. Cell Biol. 130, 639-650.
14. Brandt, R., and Lee, G. (1993). Functional organization of microtubule-associated protein tau. Identification of regions which affect microtubule growth, nucleation, and bundle formation in vitro. J. Biol. Chem. 268, 3414-3419.
15. Saurin, A.J., Borden, K.L., Boddy, M.N., and Freemont, P.S. (1996). Does this have a familiar RING? Trends Biochem. Sci. 21, 208-214.
16. Cao, T., Borden, K.L., Freemont, P.S., and Etkin, L.D. (1997). Involvement of the rfp tripartite motif in protein-protein interactions and subcellular distribution. J. Cell Sci. 110, 1563-1571.
17. Short, K.M., Hopwood, B., Yi, Z., and Cox, T.C. (2002). MID1 and MID2 homo- and heterodimerise to tether the rapamycinsensitive PP2A regulatory subunit, Alpha 4, to microtubules: Implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders. BMC Cell Biol. 3, 1.
18. Piperno, G., LeDizet, M., and Chang, X.J. (1987). Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. J. Cell Biol. 104, 289-302.
19. Takemura, R., Okabe, S., Umeyama, T., Kanai, Y., Cowan, N.J., and Hirokawa, N. (1992). Increased microtubule stability and alpha tubulin acetylation in cells transfected with microtubule-associated proteins MAP1B, MAP2 or tau. J. Cell Sci. 103, 953-964.
20. Vandre, D.D., Centonze, V.E., Peloquin, J., Tombes, R.M., and Borisy, G.G. (1991). Proteins of the mammalian mitotic spindle: Phosphorylation/dephosphorylation of MAP-4 during mitosis. J. Cell Sci. 98, 577-588.
21. Cainarca, S., Messali, S., Ballabio, A., and Meroni, G. (1999). Functional characterization of the Opitz syndrome gene product (midin): Evidence for homodimerization and association with microtubules throughout the cell cycle. Hum. Mol. Genet. 8, 1387-1396.
22. Karsenti, E. (1993). Severing microtubules in mitosis. Curr. Biol. 3, 208-210.
23. Mandato, C.A., Benink, H.A., and Bement, W.M. (2000). Microtubule-actomyosin interactions in cortical flow and cytokinesis. Cell Motil. Cytoskeleton 45, 87-92.
24. Fishkind, D.J., and Wang, Y.L. (1995). New horizons for cytokinesis. Curr. Opin. Cell Biol. 7, 23-31.
25. Chang, W., Gruber, D., Chari, S., Kitazawa, H., Hamazumi, Y., Hisanaga, S., and Bulinski, J.C. (2001). Phosphorylation of MAP4 affects microtubule properties and cell cycle progression. J. Cell Sci. 114, 2879-2887.