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Single-molecule analysis of kinesin motility reveals regulation by the cargo-binding tail domain
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Single-molecule fluorescence microscopy was used to measure frequency of microtubule-based motility of different kinesin heavy chain constructs. Truncated kinesin lacking a tail binds microtubules and moves 10-times more frequently than the full-length motor. In addition, alteration of a flexible hinge domain or a coiled-coil domain in the neck of kinesin eliminates inhibition by the tail.
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Freidman D., Vale R. Single-molecule analysis of kinesin motility reveals regulation by the cargo-binding tail domain. Nat Cell Biol. 1:1999;293-297. Single-molecule fluorescence microscopy was used to measure frequency of microtubule-based motility of different kinesin heavy chain constructs. Truncated kinesin lacking a tail binds microtubules and moves 10-times more frequently than the full-length motor. In addition, alteration of a flexible hinge domain or a coiled-coil domain in the neck of kinesin eliminates inhibition by the tail.
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(1999)
Nat Cell Biol
, vol.1
, pp. 293-297
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Freidman, D.1
Vale, R.2
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39
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0033575694
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Functional anatomy of the kinesin molecule in vivo
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This paper describes the development of an in vivo assay for kinesin function using Neurospora. Using this approach, the authors identify domains in the motor's stalk and tail that are likely to be important for cargo-binding and regulation.
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Kirchner J., Seiler S., Fuchs S., Schliwa M. Functional anatomy of the kinesin molecule in vivo. EMBO J. 18:1999;4404-4413. This paper describes the development of an in vivo assay for kinesin function using Neurospora. Using this approach, the authors identify domains in the motor's stalk and tail that are likely to be important for cargo-binding and regulation.
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(1999)
EMBO J
, vol.18
, pp. 4404-4413
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Kirchner, J.1
Seiler, S.2
Fuchs, S.3
Schliwa, M.4
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40
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0032550207
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Kinesin light chains are essential for axonal transport in Drosophila
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Gindhart J., Desai C., Beuhausen S., Zinn K., Goldstein L. Kinesin light chains are essential for axonal transport in Drosophila. J Cell Biol. 20:1998;443-454.
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(1998)
J Cell Biol
, vol.20
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Gindhart, J.1
Desai, C.2
Beuhausen, S.3
Zinn, K.4
Goldstein, L.5
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41
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0031883078
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A specific light chain of kinesin associates with mitochondria in cultured cells
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Khodjakov A., Lizunova E., Minin A., Koonce M., Gyoeva F. A specific light chain of kinesin associates with mitochondria in cultured cells. Mol Biol Cell. 9:1998;333-343.
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(1998)
Mol Biol Cell
, vol.9
, pp. 333-343
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Khodjakov, A.1
Lizunova, E.2
Minin, A.3
Koonce, M.4
Gyoeva, F.5
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42
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0031664962
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In vitro reconstitution of microtubule plus end-directed, GTPγ S-sensitive motility of Golgi membranes
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Using an in vitro motility assay that reconstitutes Golgi vesicle transport, the authors demonstrated inhibition of microtubule plus-end directed motility by GTPγS and suggested that G proteins regulate motility of Golgi membranes along microtubules.
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Fullerton A.T., Bau M.Y., Conrad P.A., Bloom G.S. In vitro reconstitution of microtubule plus end-directed, GTPγ S-sensitive motility of Golgi membranes. Mol Biol Cell. 9:1998;2699-2714. Using an in vitro motility assay that reconstitutes Golgi vesicle transport, the authors demonstrated inhibition of microtubule plus-end directed motility by GTPγS and suggested that G proteins regulate motility of Golgi membranes along microtubules.
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(1998)
Mol Biol Cell
, vol.9
, pp. 2699-2714
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Fullerton, A.T.1
Bau, M.Y.2
Conrad, P.A.3
Bloom, G.S.4
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43
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0032476645
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Overexpression of tau protein inhibits kinesin-dependent trafficking of vesicles, mitochondria, and endoplasmic reticulum: Implications for Alzheimer's disease
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•]).
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•]).
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(1998)
J Cell Biol
, vol.143
, pp. 777-794
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Ebneth, A.1
Godemann, R.2
Stamer, K.3
Illenberger, S.4
Trinczek, B.5
Mandelkow, E.6
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44
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0032799381
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Tau regulates the attachment/detachment but not the speed of motors in microtubule-dependent transport of single vesicles and organelles
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Real-time analysis of fluorescently labeled post-Golgi vesicles and mitochondria shows that Tau expression does not affect the rate of plus-end- and minus-end-directed organelle transport along microtubules but reduces the distance they travel continuously in both directions and frequency of transitions from minus-end to plus-end movement.
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Trinczek B., Ebneth A., Mandelkow E. Tau regulates the attachment/detachment but not the speed of motors in microtubule-dependent transport of single vesicles and organelles. J Cell Sci. 112:1999;2355-2367. Real-time analysis of fluorescently labeled post-Golgi vesicles and mitochondria shows that Tau expression does not affect the rate of plus-end- and minus-end-directed organelle transport along microtubules but reduces the distance they travel continuously in both directions and frequency of transitions from minus-end to plus-end movement.
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(1999)
J Cell Sci
, vol.112
, pp. 2355-2367
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Trinczek, B.1
Ebneth, A.2
Mandelkow, E.3
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45
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0032548920
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Developmental regulation of vesicle transport in Drosophila embryos: Forces and kinetics
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The movement of lipid droplets in Drosophila embryos was shown to occur bidirectionally along microtubules. Their net distribution is achieved by regulation of the amount of time spent moving in either direction (persistence time). Embryos with the klar mutation have impaired plus-end- and minus-end-directed transport, suggesting that the product of this gene may coordinate plus-end- and minus-end-directed motors bound to these organelles.
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Welte M.A., Gross S.P., Postner M., Block S.M., Wieschaus E.F. Developmental regulation of vesicle transport in Drosophila embryos: forces and kinetics. Cell. 92:1998;547-557. The movement of lipid droplets in Drosophila embryos was shown to occur bidirectionally along microtubules. Their net distribution is achieved by regulation of the amount of time spent moving in either direction (persistence time). Embryos with the klar mutation have impaired plus-end- and minus-end-directed transport, suggesting that the product of this gene may coordinate plus-end- and minus-end-directed motors bound to these organelles.
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(1998)
Cell
, vol.92
, pp. 547-557
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Welte, M.A.1
Gross, S.P.2
Postner, M.3
Block, S.M.4
Wieschaus, E.F.5
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46
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0029905303
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Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles
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Vaisberg E.A., Grissom P.M., McIntosh J.R. Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles. J Cell Biol. 133:1996;831-842.
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(1996)
J Cell Biol
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Vaisberg, E.A.1
Grissom, P.M.2
McIntosh, J.R.3
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