The light subunit of system bo,+ is fully functional in the absence of the heavy subunit

EMBO Journal

Volumn 21, Issue 18, 2002, Pages 4906-4914

  Reig, Nùria ^a   Chillarón, Josep ^a   Bartoccioni, Paola ^a   Fernández, Esperanza ^a   Bendahan, Annie ^a   Zorzano, Antonio ^a   Kanner, Baruch ^a   Palacín, Manuel ^a   Bertran, Joan ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Bo,+AT reconstitution; Bo,+AT rBAT exchanger; Cystinuria; Reabsorption; Transport asymmetry

Indexed keywords

AMINO ACID; GLYCOPROTEIN; LIPOSOME; PROTEIN SUBUNIT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME SUBSTRATE; GLYCOSYLATION; HELA CELL; HUMAN; HUMAN CELL; KINETICS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN TRANSPORT;

AMINO ACID TRANSPORT SYSTEMS; AMINO ACID TRANSPORT SYSTEMS, BASIC; ANIMALS; ARGININE; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CYSTINE; CYSTINURIA; DOGS; HELA CELLS; HUMANS; KIDNEY; LEUCINE; LIPOSOMES; MEMBRANE GLYCOPROTEINS; PROTEIN SUBUNITS;

ANIMALIA;

EID: 0037119974     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf500     Document Type: Article

References (49)

1. Abumrad, N., Harmon, C. and Ibrahimi, A. (1998) Membrane transport of long-chain fatty acids: evidence for a facilitated process. J. Lipid Res.39, 2309-2318.
2. −. Pflügers Arch., 442, 286-296.
3. Bertran, J., Werner, A., Moore, M.L., Stange, G., Markovich, D., Biber, J., Testar, X., Zorzano, A., Palacín, M. and Murer, H. (1992) Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine, dibasic and neutral amino acids. Proc. Natl Acad. Sci. USA, 89, 5601-5605.
4. Bruce, L.J., Groves, J.D., Okubo, Y., Thilaganathan, B. and Tanner, M.J. (1994) Altered band 3 structure and function in glycophorin A- and B-deficient (MkMk) red blood cells. Blood, 84, 916-922.
5. Busch, A.E., Herzer, T., Waldegger, S., Schmidt, F., Palacín, M., Biber, J., Markovich, D., Murer, H. and Lang, F. (1994) Opposite directed currents induced by the transport of dibasic and neutral amino acids in Xenopus oocytes expressing the protein rBAT. J. Biol. Chem., 269, 25581-25586.
6. Calonge, M.J. et al. (1994) Cystinuria caused by mutations in rBAT, a gene involved in the transport of cystine. Nature Genet., 6, 420-425.
7. Chairoungdua, A. et al. (1999) Identification of an amino acid transporter associated with the cystinuria-related Type II membrane glycoprotein. J. Biol. Chem., 274, 28845-28848.
8. +L-like. A tertiary active transport mechanism for renal reabsorption of cystine and dibasic amino acids. J. Biol. Chem., 271, 17761-17770.
9. Chillarón, J., Estévez, R., Samarzija, I., Waldegger, S., Testar, X., Lang, F., Zorzano, A., Busch, A. and Palacín, M. (1997) An intracellular trafficking defect in type I cystinuria rBAT mutants M467T and M467K. J. Biol. Chem., 272, 9543-9549.
10. Chillarón, J., Roca, R., Valencia, A., Zorzano, A. and Palacín, M. (2001) Heteromeric amino acid transporters: biochemistry, genetics and physiology. Am. J. Physiol. Renal. Physiol., 281, F995-F1018.
11. Coady, M.J., Jalal, F., Chen, X., Lemay, G., Berteloot, A. and Lapointe, J.Y. (1994) Electrogenic amino acid exchange via the rBAT transporter. FEBS Lett., 356, 174-178.
12. Coady, M.J., Chen, X.Z. and Lapointe, J.Y. (1996) rBAT is an amino acid exchanger with variable stoichiometry. J. Membr. Biol., 149, 1-8.
13. Devés, R. and Boyd, C.A. (2000) Surface antigen CD98(4F2): not a single membrane protein, but a family of proteins with multiple functions. J. Membr. Biol., 173, 165-177.
14. o,+AT) of rBAT. Nature Genet., 23, 52-57.
15. o,+AT heterodimer is the main apical reabsorption system for cystine in kidney. Am. J. Physiol. Renal Physiol., 283, F540-F548.
16. Folch, J., Lees, M. and Sloane Stanley, G.H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226, 497-509.
17. Font, M. et al. (International Cystinuria Consortium) (2001) Functional analysis of mutations in SLC7A9 and genotype/phenotype correlation in non-Type I cystinuria. Hum. Mol. Genet., 10, 305-316.
18. Frimpter, G.W., Horwith, M., Furth, E., Fellows, R.E. and Thompson, D.D. (1962) Inulin and endogenous amino acid renal clearances in cystinuria: evidence for tubular secretion. J. Clin. Invest., 41281-288.
19. +-independent neutral amino acid transporter that associates with the 4F2 heavy chain and exhibits substrate selectivity for small neutral D- and L-amino acids. J. Biol. Chem., 275, 9690-9698.
20. Geering, K. (2001) The functional role of beta subunits in oligomeric P-type ATPases. J. Bioenerg. Biomembr., 33, 425-438.
21. Kagawa, Y. and Racker, E. (1971) Partial resolution of the enzymes catalyzing oxidative phosphorylation. J. Biol. Chem., 246, 5477-5487.
22. Kanai, Y., Segawa, H., Miyamoto, K., Uchino, H., Takeda, E. and Endou, H. (1998) Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J. Biol. Chem., 273, 23629-23632.
23. +L neutral and basic amino acid transporter. Insights into the mechanisms of substrate recognition. J. Biol. Chem., 275, 20787-20793.
24. Kirk, P., Wilson, M.C., Heddle, C., Brown, M.H., Barclay, A.N. and Halestrap, A.P. (2000) CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO J., 19, 3896-3904.
25. Malathi, P., Preiser, H., Fairclough, P., Malett, P. and Crane, R.K. (1979) A rapid method for the isolation of kidney brush border membranes. Biochim. Biophys. Acta, 554, 259-263.
26. Mastroberardino, L., Spindler, B., Pfeiffer, R., Skelly, P.J., Loffing, J., Shoemaker, C.B. and Verrey, F. (1998) Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family. Nature, 395, 288-291.
27. Meier, C., Ristic, Z., Klauser, S. and Verrey, F. (2002) Activation of system L heterodimeric amino acid exchangers by intracellular substrates. EMBO J., 21, 580-589.
28. Mizoguchi, K. et al. (2001) Human cystinuria-related transporter: Localization and functional characterization. Kidney Int., 59, 1821-1833.
29. Mykkanen, J. et al. (2000) Functional analysis of novel mutations of y+LAT-1 amino acid transporter gene causing lysinuric protein intolerance (LPI). Hum. Mol. Genet., 9, 431-438.
30. +-independent transporter for small neutral amino acids that transport D-serine with high affinity. Neurosci. Lett., 287, 231-235.
31. Palacín, M., Borsani, G. and Sebastio, G. (2001a) The molecular bases of cystinuria and lysinuric protein intolerance. Curr. Opin. Genet. Dev., 11, 328-335.
32. Palacín, M., Goodyer, P., Nunes, V. and Gasparini, P. (2001b) Cystinuria. In Scriver, C.R., Beaudet, A.L., Sly, S.W. and Valle, D. (eds), Metabolic and Molecular Bases of Inherited Diseases. 8th edn. McGraw-Hill, New York, pp. 4909-4932.
33. +)L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter family. EMBO J., 18, 49-57.
34. Pfeiffer, R., Loffing, J., Rossier, G., Bauch, C., Meier, C., Eggermann, T., Loffing-Cueni, D., Kuhn, L.C. and Verrey, F. (1999b) Luminal heterodimeric amino acid transporter defective in cystinuria. Mol. Biol. Cell, 10, 4135-4147.
35. Pineda, M., Fernández, E., Torrents, D., Estévez, R., Lopez, C., Camps, M., Lloberas, J., Zorzano, A. and Palacín, M. (1999) Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids. J. Biol. Chem., 274, 19738-19744.
36. Pines, G., Danbolt, N.C., Bjoras, M., Zhang, Y., Bendahan, A., Eide, L., Koepsell, H., Storm-Mathisen, J., Seeberg, E. and Kanner, B.I. (1992) Cloning and expression of a rat brain L-glutamate transporter. Nature, 360, 464-467.
37. Radian, R. and Kanner, B.I. (1985) Reconstitution and purification of the sodium- and chloride-coupled γ-aminobutyric acid transporter from rat brain. J. Biol. Chem., 260, 11859-11865.
38. Rossier, G., Meier, C., Bauch, C., Summa, V., Sordat, B., Verrey, F. and Kuhn, L.C. (1999) LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine. J. Biol. Chem., 274, 34948-34954.
39. Sato, H., Tamba, M., Ishii, T. and Bannai, S. (1999) Cloning and expression of a plasma membrane cystine/glutamate exchange transporter composed of two distinct proteins. J. Biol. Chem., 274, 11455-11448.
40. −. Antioxid. Redox Signal., 2665-671.
41. +-independent neutral amino acid transporter with broad substrate selectivity. J. Biol. Chem., 274, 19745-19751.
42. Silbernagl, S. (1988) The renal handling of amino acids and oligopeptides. Physiol. Rev., 68, 911-1007.
43. o,+-like system in chicken brush border jejunum. J. Membr. Biol., 180, 213-220.
44. +L. A candidate gene for lysinuric protein intolerance. J. Biol. Chem., 273, 32437-32445.
45. Verrey, F., Jack, D.L., Paulsen, I.T., Saier, M.H., Jr and Pfeiffer, R. (1999) New glycoprotein-associated amino acid transporters. J. Membr. Biol., 172, 181-192.
46. Verrey, F., Meier, C., Rossier, G. and Kuhn, L.C. (2000) Glycoprotein-associated amino acid exchangers: broadening the range of transport specificity. Pflügers Arch., 440, 503-512.
47. Wagner, C.A., Lang, F. and Bröer, S. (2001) Function and structure of heterodimeric amino acid transporters. Am. J. Physiol. Cell Physiol., 281, C1077-C1093.
48. Wang, Y. and Tate, S.S. (1995) Oligomeric structure of a renal cystine transporter: implications in cystinuria. FEBS Lett., 368, 389-392.
49. Wilson, M.C., Meredith, D. and Halestrap, A.P. (2002) Fluorescence resonance energy transfer studies on the interaction between the lactate transporter MCT1 and CD147 provide information on the topology and stoichiometry of the complex in situ. J. Biol. Chem., 277, 3666-3672.