Physical methods to determine the binding mode of putative ligands for hepatitis C virus NS3 helicase

Analytical Biochemistry

Volumn 309, Issue 2, 2002, Pages 186-195

  Sarver, Ronald W ^a   Rogers, Joseph M ^a   Stockman, Brian J ^a   Epps, Dennis E ^b   DeZwaan, Jack ^a   Harris, Melissa S ^a   Baldwin, Eric T ^a  

^a Pharmacia and Upjohn   (United States)

^b PFIZER INC   (United States)

Author keywords

Calorimetry; Circular dichroism; Hepatitis C virus helicase; Inhibitor; Nuclear magnetic resonance; X ray crystallography

Indexed keywords

BINDING ENERGY; CALORIMETERS; CALORIMETRY; DENATURATION; DICHROISM; DISSOCIATION; ENZYMES; EQUILIBRIUM CONSTANTS; ISOTHERMS; MOLECULES; NUCLEAR MAGNETIC RESONANCE; OLIGONUCLEOTIDES; VIRUSES;

DISSOCIATION CONSTANT; EQUILIBRIUM DISSOCIATION CONSTANT; HELICASES; HIGH-THROUGHPUT SCREENING; INHIBITOR; ISOTHERMAL TITRATION CALORIMETRY; NON-SPECIFIC BINDING; NONSTRUCTURAL PROTEINS;

X RAY CRYSTALLOGRAPHY;

ENZYME INHIBITOR; HELICASE; HELICASE INHIBITOR; NONSTRUCTURAL PROTEIN 3; OLIGONUCLEOTIDE; UNCLASSIFIED DRUG;

ARTICLE; CALORIMETRY; DISSOCIATION CONSTANT; DRUG DETERMINATION; DRUG SCREENING; ENZYME ASSAY; ENZYME BINDING; ENZYME DENATURATION; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; HEPATITIS C VIRUS; ISOTHERM; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; TITRIMETRY;

HEPATITIS C VIRUS;

EID: 0037109691     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00301-9     Document Type: Article

References (19)

1. D.E. Epps, R.W. Sarver, J.M. Rogers, J.T. Herberg, P.K. Tomich, The ligand affinity of proteins measured by isothermal denaturation kinetics, Anal. Biochem. 292 (2001) 40-50.
2. N. Boyer, P. Marcellin, Pathogenesis, diagnosis and management of hepatitis C, J. Hepatology, Suppl. 32 (2000) 98-112.
3. M.A. Walker, Hepatitis C virus: An overview of current approaches and progress, Drug Discovery Today 4 (11) (1999) 518-529.
4. H.S Cho, N.C. Ha, L.W. Kang, M.C. Kyung, H.B. Sung, K.Y. Sung, B.H. Oh, Crystal Structure of RNA helicase from genotype 1b hepatitis C virus. A feasible mechanism of unwinding duplex RNA, J. Biol. Chem. 273 (1998) 15045-15052.
5. J.L. Kim, K.A. Morgenstern, J.P. Griffith, M.D. Dwyer, J.A. Thomson, M.A. Murcko, C. Lin, P.R. Caron, Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: The crystal structure provides insights into the mode of unwinding, Structure 5 (1998) 89-100.
6. N. Yao, T. Hesson, M. Cable, Z. Hong, A.D. Kwong, H.V. Le, P.C. Weber, Structure of the hepatitis C virus RNA helicase domain, Nature Struct. Biol. 4 (1997) 463-467.
7. 2, and Poly(rU), J. Biol. Chem. 273 (1998) 7390-7396.
8. M. Houghton, Q. Choo, J. Han, J. Choe, HCV NS3 Protein Fragments having Helicase Activity and Improved Solubility, Patent US6194140.
9. C.E. Bystrom, D.W. Pettigrew, S.J. Remington, B.P. Branchaud, ATP Analogs with non-transferable groups in the γ position as inhibitors of glycerol kinase, Bioorg. Med. Chem. Lett. 20 (7) (1997) 2613-2616.
10. V.V. Plotnikov, J.M. Brandts, L. Lin, J.F. Brandts, A new ultrasensitive scanning calorimeter, Anal. Biochem. 250 (1997) 237-244.
11. E.R. Malinowski, D.G. Howery, Factor Analysis in Chemistry, Robert Krieger, Malabar, FL, 1989.
12. R.W. Sarver, W.C. Krueger, An infrared and circular dichroism combined approach to the analysis of protein secondary structure, Anal. Biochem. 199 (1991) 61-67.
13. J.P. Hennessey Jr., W.C. Johnson Jr., Information content in the circular dichroism of proteins, Biochemistry 20 (1981) 1085-1094.
14. L.A. Compton, C.W. Johnson Jr., Analysis of protein circular dichroism spectra for secondary structure using a simple matrix multiplication, Anal. Biochem. 155 (1986) 155-167.
15. P.J. Hajduk, E.T. Olejniczak, S.W. Fesik, One-dimensional relaxation- and diffusion-edited NMR methods for screening compounds that bind to macromolecules, J. Am. Chem. Soc. 119 (1997) 12257-12261.
16. L. Lin, A.B. Mason, R.C. Woodworth, J.F. Brandts, Calorimetric studies of the binding of ferric ions to ovotransferrin and interactions between binding sites, Biochemistry 30 (1991) 11660-11669.
17. G.L. Bryant, Jr., M.S. Harris, E.T. Baldwin, L. Tandeske, K.R. Shoemaker, B.C. Finzel, HCV Helicase RNA-Binding Domain Flexibility Quantified by Comparison of Multiple Crystal Forms, American Crystallographic Association Meeting, Buffalo, NY, May 22-27, 1999.
18. S.S. Velankar, P. Soultanas, M.S. Dillingham, H.S. Subramanya, D.B. Wigley, Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism, Cell 97 (1999) 75-84.
19. S.R. Sprang, G protein mechanisms: Insights from structural analysis, Annu. Rev. Biochem. 66 (1997) 639-678.