Helix-hairpin-helix motifs confer salt resistance and processivity on chimeric DNA polymerases

Proceedings of the National Academy of Sciences of the United States of America

Volumn 99, Issue 21, 2002, Pages 13510-13515

  Pavlov, Andrey R ^a   Belova, Galina I ^a   Kozyavkin, Sergei A ^a   Slesarev, Alexei I ^a  

^a Fidelity Systems Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETAINE; CHIMERIC PROTEIN; DNA POLYMERASE; DNA TOPOISOMERASE; GLUCONATE POTASSIUM; SODIUM CHLORIDE; TAQ POLYMERASE;

ARTICLE; CHIMERA; DNA BINDING; DNA SYNTHESIS; ENZYME ACTIVITY; HELIX HAIRPIN HELIX MOTIF; MATHEMATICAL MODEL; PRIORITY JOURNAL; PROTEIN MOTIF; SALT TOLERANCE; SEQUENCE ANALYSIS; THERMOSTABILITY;

AMINO ACID MOTIFS; BASE SEQUENCE; BETAINE; BINDING SITES; DNA; DNA TOPOISOMERASES, TYPE I, ARCHAEAL; DNA-DIRECTED DNA POLYMERASE; ENZYME ACTIVATION; ENZYME STABILITY; GLUTAMATES; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SODIUM CHLORIDE; SUBSTRATE SPECIFICITY; TAQ POLYMERASE; TEMPERATURE;

EID: 0037109049     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.202127199     Document Type: Article

References (26)

1. Harrison, S. C. (1991) Nature 353, 715-719.
2. Thayer, M. M., Ahern, H., Xing, D., Cunningham, R. P. & Tainer, J. A. (1995) EMBO J. 14, 4108-4120.
3. Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H. & Kraut, J. (1996) Biochemistry 35, 12742-12761.
4. Doherty, A. J., Serpell, L. C. & Ponting, C. P. (1996) Nucleic Acids Res. 24, 2488-2497.
5. Shao, X. & Grishin, N. V. (2000) Nucleic Acids Res. 28, 2643-2650.
6. Slesarev, A. I., Stetter, K. O., Lake, J. A., Gellert, M., Krah, R. & Kozyavkin, S. A. (1993) Nature 364, 735-737.
7. Slesarev, A. I., Lake, J. A., Stetter, K. O., Gellert, M. & Kozyavkin, S. A. (1994) J. Biol. Chem. 269, 3295-3303.
8. Slesarev, A. I., Belova, G. I., Lake, J. A. & Kozyavkin, S. A. (2001) Methods Enzymol. 334, 179-192.
9. Belova, G. I., Prasad, R., Kozyavkin, S. A., Lake, J. A., Wilson, S. H. & Slesarev, A. I. (2001) Proc. Natl. Acad. Sci. USA 98, 6015-6020.
10. Belova, G. I., Prasad, R., Nazimov, I. V., Wilson, S. H. & Slesarev, A. I. (2002) J. Biol. Chem. 277, 4959-4965.
11. Press, W. H., Flannery, B. P., Teukolsky, S. A. & Vetterling, W. T. (1986) Numeric Recipes: The Art of Scientific Computing (Cambridge Univ. Press, Cambridge, U.K.).
12. Boeker, E. A. (1982) Biochem. J. 203, 117-123.
13. Washington, M. T., Johnson, R. E., Prakash, S. & Prakash, L. (1999) J. Biol. Chem. 274, 36835-36838.
14. Kozyavkin, S. A., Pushkin, A. V., Eiserling, F. A., Stetter, K. O., Lake, J. A. & Slesarev, A. I. (1995) J. Biol. Chem. 270, 13593-13595.
15. Rees, W. A., Yager, T. D., Korte, J. & von Hippel, P. H. (1993) Biochemistry 32, 137-144.
16. Melchior, W. B., Jr., & Von Hippel, P. H. (1973) Proc. Natl. Acad. Sci. USA 70, 298-302.
17. Fairfield, F. R., Newport, J. W., Dolejsi, M. K. & von Hippel, P. H. (1983) J. Biomol. Struct. Dyn. 1, 715-727.
18. von Hippel, P. H., Fairfield, F. R. & Dolejsi, M. K. (1994) Ann. N.Y. Acad. Sci. 726, 118-131.
19. Villbrandt, B., Sobek, H., Frey, B. & Schomburg, D. (2000) Protein Eng. 13, 645-654.
20. Innis, M. A., Myambo, K. B., Gelfand, D. H. & Brow, M. A. (1988) Proc. Natl. Acad. Sci. USA 85, 9436-9440.
21. Li, Y., Mitaxov, V. & Waksman, G. (1999) Proc. Natl. Acad. Sci. USA 96, 9491-9496.
22. Perler, F. B., Kumar, S., & Kong, H. (1996) Adv. Protein Chem. 48, 377-435.
23. Nixon, A. E., Ostermeier, M. & Benkovic, S. J. (1998) Trends Biotechnol. 16, 258-264.
24. Beguin, P. (1999) Curr. Opin. Biotechnol. 10, 336-340.
25. Kaledin, A. S., Slyusarenko, A. G. & Gorodetskii, S. I. (1980) Biokhimiya 45, 644-651.
26. Abu Al-Soud, W, & Radstrom, P. (1998) Appl. Environ. Microbiol. 64, 3748-3753.