Improved initial yields in C-terminal sequence analysis by thiohydantoin chemistry using purified diphenylphosphoryl isothiocyanate: NMR evidence for a reaction intermediate in the coupling reaction

Analytical Biochemistry

Volumn 307, Issue 2, 2002, Pages 202-211

  Graham, Kenneth ^a   Shively, John E ^a  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

C terminal sequence analysis; Diphenylphosphoryl isothiocyanate; Thiohydantoin

Indexed keywords

AMINO ACIDS; CARBOXYLATION; CHEMICAL BONDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PEPTIDES; PURIFICATION; REAGENTS;

C-TERMINAL SEQUENCES; CARBOXYL GROUPS; COUPLING MECHANISM; COUPLING REACTION; ISOTHIOCYANATES; REVERSED PHASE HPLC; SPECTROSCOPIC EVIDENCE; THIOHYDANTOIN;

REACTION INTERMEDIATES;

AMINO ACID; BENZENE; CARBOXYLIC ACID; DIPHENYLPHOSPHORYL ISOTHIOCYANATE; ISOTHIOCYANIC ACID DERIVATIVE; PEPTIDYLTHIOHYDANTOIN DERIVATIVE; POTASSIUM DERIVATIVE; POTASSIUM TRIMETHYLSILANOLATE; PYRIDINE; THIOHYDANTOIN DERIVATIVE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHEMICAL REACTION; DERIVATIZATION; MOLECULAR STABILITY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTON NUCLEAR MAGNETIC RESONANCE; REACTION ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEQUENCE ANALYSIS; SYNTHESIS;

EID: 0037102188     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00025-8     Document Type: Article

References (43)

1. A protein sequenator
2. A gas-liquid solid-phase peptide and protein sequenator
3. Caspases: The proteases of the apoptotic pathway
4. Prodomains-adaptors-oligomerization: The pursuit of caspase activation in apoptosis
5. Caspases: Key mediators of apoptosis
6. Proteases, proteolysis, apoptosis
7. Role for caspase mediated cleavage of rad 51 in induction of apoptosis by DNA damage
8. Caspase-mediated cleavage of DNA topoisomerase I at unconventional sites during apoptosis
9. Caspase-mediated cleavage of p21Waf1/Cip1 converts cancer cells from growth arrest to undergoing apoptosis
10. Identification of caspases that cleave presenilin-1 and presenilin-2: Five presenilin-1 (PSI) mutations do not alter the sensitivity of PSI to caspases
11. Carboxyl-terminal fragments of presenilin-1 are closely related to cytoskeletal abnormalities in Alzheimer's brains
12. Phosphorylation of presenilin-2 regulates its cleavage by caspases and retards progression of apoptosis
13. Proteolytic processing of the Alzheimer's disease amyloid precursor protein within its cytoplasmic domain by caspase-like proteases
14. Efficient sequence analysis of six gene products (7-74kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high resolution mass spectrometry
15. Purification and characterization of serine acetyltransferase from Escherichia coli partially truncated at the C-terminal region
16. Sequence properties of GPI-anchored proteins near the omega-site: Constraints for the polypeptide binding site of the putative transamidase
17. Carboxy-terminal processing of the urokinase receptor: Implication for substrate recognition and glycosylphosphatidylinositol anchor addition
18. Mammalian GPI proteins: Sorting, membrane residence and functions
19. GPI-anchor biosynthesis
20. How glycosylphosphatidylinositol-anchored membrane proteins are made
21. PIG-tailed membrane proteins
22. Cloning and characterization of a mammalian prenyl protein specific protease
23. Structure characterization by tandem mass spectrometry of the posttranslational polyglycation of tubulin
24. Multiple forms of tubulin: Different gene products and covalent modifications
25. Uber eine neue methode sur ermittlung der konstitution von peptiden
26. Chemical procedures for C-terminal sequencing of peptides and proteins
27. Chemical methods of protein sequence analysis
28. Studies in thiohydantoin chemistry. 2. C-terminal sequencing of peptides
29. Studies in thiohydantoin chemistry. 1. Some aspects of the Schlack-Kumpf reaction
30. Sequencing of peptides and proteins from the carboxy terminus
31. Activation of the carboxy-terminus of a peptide for carboxy-terminal sequencing
32. Carboxy-terminal sequencing-formation and hydrolysis of C-terminal peptidylthiohydantoins
33. Automated carboxy-terminal sequence analysis of peptides
34. Automated carboxy-terminal sequence analysis of peptides and proteins using diphenyl phosphorisothiocyanatidate
35. Automated carboxy-terminal sequence analysis of polypeptides containing C-terminal proline
36. Peptides. IV. Selective removal of the C-terminal residue as a thiohydantoin: The use of diphenyl horisothiocyanatidate
37. Electro-blotting of proteins to Teflon tape acid membranes for N-terminal and C-terminal sequence analysis
38. Chemical carboxy-terminal sequence analysis of peptides using acetyl isothiocyanate
39. A method for preparation of amino acid thiohydantoins from free amino acids activated by acetyl chloride for development of protein C-terminal sequencing
40. H-1, C-13 and N-15 chemical-shift referencing in biomolecular NMR
41. High sensitivity analysis of phenylthiohydanotin amino acid derivatives by electrospray mass spectrometry
42. Diphenylphosphoryl azide: A new convenient reagent for a modified Curtis reaction and for peptide synthesis