Analysis of oligosaccharide structures of glycoproteins in polyacrylamide gel

Analytical Biochemistry

Volumn 303, Issue 2, 2002, Pages 206-209

  Nakakita, Shin ichi ^a   Ama, Daisuke ^a   Natsuka, Shunji ^a   Hase, Sumihiro ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

OLIGOSACCHARIDE STRUCTURE; POLYACRYLAMIDE GELS;

CHEMISTRY;

GLYCOPROTEIN; OLIGOSACCHARIDE; POLYACRYLAMIDE GEL;

ANALYTIC METHOD; ANIMAL TISSUE; BRAIN TISSUE; CARBOHYDRATE ANALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; MOUSE; NONHUMAN; NOTE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

ANIMALIA;

EID: 0037089473     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5583     Document Type: Article

References (11)

1. Sequencing of N-linked oligosaccharides directly from protein gels: In-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography
2. Monosaccharide and oligosaccharide analysis of proteins transferred to polyvinylidene fluoride membranes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis
3. Characterization of carbohydrates using highly fluorescent 2-aminobenzoic acid tag following gel electrophoresis of glycoproteins
4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
5. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
6. Analysis of N- and O-glycans by pyridylamination
7. Release of O-linked sugar chains from glycoproteins with anhydrous hydrazine and pyridylamination of the sugar chains with improved reaction conditions
8. A pyridylamination method aimed at automatic oligosaccharide analysis of N-linked sugar chains
9. The asparagine-linked oligosaccharides on bovine fetuin
10. Presence of an O-glycosidically linked hexasaccharide in fetuin
11. Development-dependent expression of complex-type sugar chains specific to mouse brain