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The diameter used here is the average of the value estimated from the collapse point of PLGA (1.1 nm) and the CPK molecular model of PLGA in the all-trans conformation (1.3 nm).
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A small jump-in of 1-2 nm is detectable under the current condition, because (1) we performed the SFA measurement by the conventional method, and (2) the jump-in occurs against a relatively large repulsion. The former means that we stopped at each separation distance and moved to a next separation manually with observing the FECO fringes by eyes. The latter means that we needed to move the motor to a considerable extent: when we move the surface by i nm against the repulsion with a decay length of 28 nm, we need to drive the motor drive by ca. 10 nm at F/R ∼ 1 mN/m. Thus it is difficult to fail to observe "jump-in", because we first detected the "jump-in" by eyes and then confirmed that it was also visible in the plotted data.
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