Modification of an essential amino group in the mineralocorticoid receptor evidences a differential conformational change of the receptor protein upon binding of antagonists, natural agonists and the synthetic agonist 11,19-oxidoprogesterone

Biochimica et Biophysica Acta - Molecular Cell Research

Volumn 1589, Issue 1, 2002, Pages 31-48

  Piwien Pilipuk, Graciela ^a   Kanelakis, Kimon C ^a   Ghini, Alberto A ^b   Lantos, Carlos P ^b   Litwack, Gerald ^c   Burton, Gerardo ^b   Galigniana, Mario D ^a,b  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^c THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

2,4,6 Trinitrobenzenesulfonate; Aldosterone; hsp90 heterocomplex; Pyridoxal 5 phosphate; Sodium retention

Indexed keywords

11,19 OXIDOPROGESTERONE; 21 DEOXYPREGNANESTEROID 11,19 OXIDOPROGESTERONE; 6,19 OXIDOPROGESTERONE; ALDOSTERONE; ALKYLATING AGENT; AMINO ACID; CHYMOTRYPSIN A; CORTICOSTERONE; DEOXYCORTICOSTERONE; HEAT SHOCK PROTEIN 90; LIGAND; LYSINE; MINERALOCORTICOID ANTAGONIST; MINERALOCORTICOID RECEPTOR; PROGESTERONE DERIVATIVE; PYRIDOXAL 5 PHOSPHATE; RECEPTOR PROTEIN; SPIRONOLACTONE; TESTOSTERONE; TRINITROBENZENESULFONIC ACID; UNCLASSIFIED DRUG;

ALKYLATION; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; BINDING SITE; CHEMICAL MODIFICATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; INHIBITION KINETICS; LIGAND BINDING; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; RAT; RECEPTOR BINDING; STEROID BINDING;

ALDOSTERONE; ALKYLATING AGENTS; AMINES; ANIMALS; BINDING SITES; CHYMOTRYPSIN; CYTOSOL; HSP90 HEAT-SHOCK PROTEINS; HYDROGEN-ION CONCENTRATION; KIDNEY; MALE; PROGESTERONE; PROTEIN CONFORMATION; PYRIDOXAL PHOSPHATE; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, MINERALOCORTICOID; TIME FACTORS; TRINITROBENZENESULFONIC ACID; TRITIUM;

EID: 0037070088     PISSN: 01674889     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4889(01)00184-7     Document Type: Article

References (49)

1. The steroid and thyroid hormone receptor superfamily
2. Steroid receptor interactions with heat shock protein and immunophilin chaperones
3. Effects of calf intestinal alkaline phosphatase, phosphatase inhibitors and phosphorylated compounds on the rate of activation of glucocorticoid receptor complexes
4. Phosphorylation of steroid hormone receptors
5. Inhibition of glucocorticoid receptor nucleocytoplasmic shuttling by okadaic acid requires intact cytoskeleton
6. Geldanamycin, a heat shock protein 90-binding benzoquinone ansamycin, inhibits steroid dependent translocation of the glucocorticoid receptor from cytoplasm to the nucleus
7. Heat shock protein 90-dependent (geldanamycin-inhibited) movement of the glucocorticoid receptor through the cytoplasm to the nucleus requires intact cytoskeleton
8. Different regions of the immunophilin FKBP52 determine its association with the glucocorticoid receptor, hsp90, and cytoplasmic dynein
9. Evidence that the peptidylprolyl isomerase (PPIase) domain of the hsp90-binding immunophilin FKBP52 is a dynein interaction domain involved in glucocorticoid receptor movement to the nucleus
10. Mineralocorticoid action: Target tissue specificity is enzyme, not receptor, mediated
11. Features of the shuttle pair 11β-hydroxyprogesterone/11-keto-progesterone
12. Demonstration of nuclear translocation of the mineralocorticoid receptor (MR) using an anti-MR antibody and confocal scanning microscopy
13. Differential intracellular localization of the human mineralocorticoid receptor on binding of agonists and antagonists
14. Tautomycin inhibits phosphatase-dependent transformation of the rat kidney mineralocorticoid receptor
15. The steroid binding domain influences intracellular solubility of the baculovirus overexpressed glucocorticoid and mineralocorticoid receptors
16. Distinct functions of the hsp90 kDa heat-shock protein (hsp90) in oestrogen and mineralocorticoid receptor activity: Effects of hsp90 deletion mutants
17. Native rat kidney mineralocorticoid receptor is a phosphoprotein whose transformation to a DNA-binding form is induced by phosphatases
18. Comparative inhibition by hard and soft metal ions of steroid-binding capacity of renal mineralocorticoid receptor cross-linked to the 90-kDa heat shock protein heterocomplex
19. The unliganded mineralocorticoid receptor is associated with heat shock proteins 70 and 90 and the immunophilin FKBP-52
20. Overexpression and characterization of the human mineralocorticoid receptor
21. Functional regulation of corticosteroid receptors by phosphorylation and redox potential
22. Protein phosphatases type and/or type 2A regulate nucleocytoplasmic shuttling of glucocorticoid receptors
23. Stability study on renal type I mineralocorticoid receptor
24. Sulfhydryl groups are involved in the binding of agonists and antagonists to the human mineralocorticoid receptor
25. Cysteines 849 and 942 of human mineralocorticoid receptor are crucial for steroid binding
26. Oxidative stress induced by L-buthionine-(S,R)-sulfoximine, a selective inhibitor of glutathione metabolism, abrogates mouse kidney mineralocorticoid function
27. Mechanism of action of the potent sodium-retaining steroid 11,19-oxidoprogesterone
28. An improved preparation of 11,19-oxidopregn-4-ene-3,20-dione and 6,19-oxidopregn-4-ene-3,11,20-trione
29. Pyridoxal phosphate. An anionic probe for protein amino groups exposed on the outer and inner surfaces of intact human red blood cells
30. Characteristics of papillary collecting duct cells in primary culture
31. Sodium-retaining activity of some natural and synthetic 21-deoxysteroids
32. Ligand properties of 11,19-oxidoprogesterone. A preliminary report
33. Evidence for an essential lysyl group residue in phospholipase D from Streptomyces sp. by modification with diethyl pyrocarbonate and pyridoxal 5-phosphate
34. Determination of free aminogroups in proteins by trinitrobenzenesulfonic acid
35. Inactivation of E. coli RNA polymerase by pyridoxal 5′-phosphate: Identification of a low pKa lysine as the modified residue
36. Control of estrogen receptor ligand binding by hsp90
37. 11,19-Oxidoprogesterone: A potent sodium retaining steroid binding to an alternative site in kidney type I receptor
38. Renal mineralocorticoid receptors and hippocampal corticosterone binding species have identical intrinsic steroid specificity
39. Specificity in mineralocorticoid versus glucocorticoid action
40. Molecular cloning of a mineralocorticoid (type I) receptor complementary DNA from rat hippocampus
41. Hormone and antihormone induce distinct conformational changes which are central to steroid receptor activation
42. Transcriptional activation by the estrogen receptor requires a conformational change in the ligand-binding domain
43. Different agonist- and antagonist-induced conformational changes in retinoic acid receptors analyzed by protease mapping
44. Activating mineralocorticoid receptor mutation in hypertension exacerbated by pregnancy
45. A single amino acid mutation of Ala-773 in the mineralocorticoid receptor confers agonist properties to 11β-substituted spirolactones
46. Mechanism of action of aldosterone blockers
47. Autoradiographic evidence of nuclear binding of spironolactone in rabbit cortical collecting tubule
48. Characterization of the interaction of the human mineralocorticoid receptor with hormone response elements
49. Cell-specific, promoter-dependent mineralocorticoid agonist activity of spironolactone