Production and characterisation of a recombinant scfv reactive with human gastrointestinal carcinomas

British Journal of Cancer

Volumn 87, Issue 4, 2002, Pages 405-413

  Kim, D J ^a   Chung, J H ^a   Ryu, Y S ^a   Rhim, J H ^a   Kim, C W ^a   Suh, Y ^a   Chung, H K ^a  

^a Seoul National University College of Medicine   (South Korea)

Author keywords

Expression cloning; Sc142 antibody; Sc142 scfv; Sc142 reactive antigen

Indexed keywords

RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; CANCER DIAGNOSIS; CANCER IMMUNOTHERAPY; CONTROLLED STUDY; DISSOCIATION CONSTANT; ESCHERICHIA COLI; GASTROINTESTINAL CARCINOMA; GENE TECHNOLOGY; HUMAN; HUMAN CELL; HYBRIDOMA CELL CULTURE; IMAGING; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; SERODIAGNOSIS;

ADENOCARCINOMA; AMINO ACID SEQUENCE; ANTIGENS, NEOPLASM; ESCHERICHIA COLI; GASTROINTESTINAL NEOPLASMS; HUMANS; HYBRIDOMAS; MEMBRANE GLYCOPROTEINS; RECOMBINANT PROTEINS; STOMACH NEOPLASMS;

EID: 0037068363     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjc.6600365     Document Type: Article

References (24)

1. Immunological and structural characterization of a high affinity anti-fluorescein single-chain antibody
2. Purification and characterization of a peanut-agglutinin-binding pancreatic-cancer-related serum mucus glycoprotein
3. Antibody humanization: A case of the 'Emperor's new clothes'
4. A human model of gastric carcinogenesis
5. The use of equilibrium-density-gradient methods for the preparation and characterization of blood-group-specific glycoproteins
6. Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas
7. Determination of relative binding affinity of influenza virus N9 sialidases with the Fab fragment of monoclonal antibody NC41 using biosensor technology
8. A critique of the evidence for active host defence against cancer, based on personal studies of 27 murine tumours of spontaneous origin
9. A new tumor-associated antigen of gastrointestinal carcinoma defined by monoclonal antibody SC142
10. Creating and engineering human antibodies for immunotherapy
11. Sequence heterogeneity in Ig kappa transcripts from single B lymphocytes
12. Isolation and properties of a human pancreatic adenocarcinoma-associated antigen, DU-PAN-2
13. Phage-display library selection of high-affinity human single-chain antibodies to tumor-associated carbohydrate antigens sialyl Lewisx and Lewisx
14. Radioimmunoguided surgery in colorectal cancer using a genetically engineered anti-CEA single-chain Fv antibody
15. Phage antibodies: Filamentous phage displaying antibody variable domains
16. Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli
17. Construction, expression and characterisation of a single-chain diabody derived from a humanised anti-Lewis Y cancer targeting antibody using a heat-inducible bacterial secretion vector
18. Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent - Application to a human single-chain Fv fragment
19. Mucin genes: Structure, expression and regulation
20. Selective inhibition of tumor cell growth by a recombinant single-chain antibody-toxin specific for the erbB-2 receptor
21. Man made antibodies
22. Expression of nm23-H1 gene and Sialyl Lewis X antigen in breast cancer
23. Expression and characterization of anti-NCA-95 scFv (CEA 79 scFv) in a prokaryotic expression vector modified to contain a SfiI and NotI site