Non-classical nuclear localization signal peptides for high efficiency lipofection of primary neurons and neuronal cell lines

Neuroscience

Volumn 112, Issue 1, 2002, Pages 1-5

  Ma, H ^a   Zhu, J ^a   Maronski, M ^a   Kotzbauer, P T ^b   Lee, V Y ^b   Dichter, M A ^a   Diamond, S L ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Hippocampal neurons; NT2 neuron; Retinal ganglion cells; Transportin; Ventral mesencephalon neuron

Indexed keywords

KARYOPHERIN; RIBONUCLEOPROTEIN; SIGNAL PEPTIDE;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL LINE; CELL NUCLEUS; CELL STRAIN; CELLULAR DISTRIBUTION; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSFECTION; HIPPOCAMPUS; HUMAN; HUMAN CELL; LIPOFECTION; MESENCEPHALON; MITOSIS; NERVE CELL; NONHUMAN; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN TARGETING; RAT; RETINA GANGLION CELL;

ANIMALS; CELL LINE; CELL NUCLEUS; CHICK EMBRYO; CULTURE TECHNIQUES; EMBRYO; HIPPOCAMPUS; HUMANS; LIPOSOMES; NEURONS; PROTEIN SORTING SIGNALS; RATS; RATS, SPRAGUE-DAWLEY; TISSUE DISTRIBUTION; TRANSFECTION;

EID: 0037067045     PISSN: 03064522     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0306-4522(02)00044-1     Document Type: Article

References (17)

1. Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems
2. Adenovirus in the brain: Recent advances of gene therapy for neurodegenerative diseases
3. Combined use of the green and yellow fluorescent proteins and fluorescence-activated cell sorting to select populations of transiently transfected PC12 cells
4. Single-cell electroporation for gene transfer in vivo
5. Functional synapses are formed between human NTera2 (NT2N, hNT) neurons grown on astrocytes
6. Efficient expression of tetracycline-responsive gene after transfection of dentate gyrus neurons in vitro
7. Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons
8. A nuclear export signal in hnRNP A1: A signal-mediated, temperature-dependent nuclear protein export pathway
9. Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector
10. Nuclear targeting peptide scaffolds for lipofection of nondividing mammalian cells
11. Inducibility of BDNF gene promoter I detected by calcium-phosphate-mediated DNA transfection is confined to neuronal but not to glial cells
12. Mitosis enhances transgene expression of plasmid delivered by cationic liposomes
13. Adeno-associated virus mediated gene transfer into primary rat brain neuronal and glial cultures: Enhancement with the pH-sensitive surfactant dodecyl 2-(1′-imidazolyl) propionate
14. Calcium phosphate-mediated transfection of primary cultured brain neurons using GFP expression as a marker: Application for single neuron electrophysiology
15. Optimized protocol for biolistic transfection of brain slices and dissociated cultured neurons with a hand-held gene gun
16. Lipid-mediated gene transfer into primary neurons using FuGene: Comparison to C6 glioma cells and primary glia
17. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism