Spatial relationship between the prodan site, Trp-214, and Cys-34 residues in human serum albumin and loss of structure through incremental unfolding

Biochemistry

Volumn 41, Issue 23, 2002, Pages 7443-7452

  Krishnakumar, Shyam Sundar ^a   Panda, Dulal ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY BOMBAY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

SERUM ALBUMIN;

DRUG DOSAGE; ENERGY TRANSFER; FLUORESCENCE;

BIOCHEMISTRY;

ALBUMIN; COUMARIN DERIVATIVE; CYSTEINE; GUANIDINE; NAPHTHALENE DERIVATIVE; PALMITIC ACID; PRODAN; TRYPTOPHAN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; FLUORESCENCE; HUMAN; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE;

2-NAPHTHYLAMINE; BINDING SITES; BINDING, COMPETITIVE; CYSTEINE; ENERGY TRANSFER; FLUORESCENT DYES; HEAT; HUMANS; PALMITIC ACID; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; SERUM ALBUMIN; SPECTROMETRY, FLUORESCENCE; TRYPTOPHAN; WARFARIN;

EID: 0037062557     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi025699v     Document Type: Article

References (54)

1. Atomic structure and chemistry of human serum albumin
2. Structure of serum albumin
3. The characterization of two specific drug binding sites on human serum albumin
4. Binding of drugs to human serum albumin. XI. The specificity of three binding sites as studied with albumin immobilized in microparticles
5. Evidence for a large and flexible region of human serum albumin possessing high affinity binding sites for salicylate, warfarin, and other ligands
6. Characterization of drug binding site 1 on human serum albumin: Concept about the structure of a drug-binding site Biochim
7. Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites
8. Crystallographic analysis reveals common modes of binding of medium and long-chain fatty acids to human serum albumin
9. Long chain fatty acid binding to human plasma albumin
10. Molecular aspects of ligand binding to serum albumin
11. Dietary influences on serum lipids and lipoproteins
12. Probing the Cysteine-34 residue in human serum albumin by fluorescent techniques
13. Folding of protein fragments
14. Protein misassembly in vitro
15. Structural studies and organic ligand-binding properties of bovine plasma albumin
16. Fragments of bovine serum albumin produced by limited proteolysis - Conformation and ligand binding
17. Secretory expression of human albumin domains in Saccharomyces cerevisiae and their binding of myristic acid and an acylated insulin analogue
18. Thermodynamic aspects of the thermal stability of human serum albumin
19. Molten globule-like state of human serum albumin at low pH
20. Experimental determination of the free energy of unfolding of proteins
21. Anion-induced stabilization of human serum albumin prevents the formation of intermediate during urea denaturation
22. Unfolding of acrylodan-labeled human serum albumin probed by steady state and time-resolved fluorescence methods
23. Serum albumin: Amino acid sequence
24. Human serum albumin spectroscopic studies of binding and proximity relationship for fatty acids and bilirubin
25. Structural features of the hydroxy- and keto disubstituted bile salts: Human serum albumin binding
26. Structural specificity requirements in the binding of beta lactam antibiotics to human serum albumin
27. Reversible dimer dissociation of tubulin S and tubulin detected by fluorescence anisotropy
28. Single-molecule protein folding diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2
29. Translocation of sigma (70) with RNA polymerase during transcription: Fluorescence resonance energy transfer assay for movement relative to DNA
30. Resonance energy transfer between cysteine-34 and tryptophan-214 in human serum albumin-distance measurements as a function of pH
31. Synthesis and spectral properties of a hydrophobic fluorescent probe: 6-propionyl-2-(dimethylamino) naphthalene
32. Estimation of polarity of protein interior by optical spectroscopy
33. Polar pocket with nonpolar lining
34. Stabilization of microtubule dynamics by estramustine by binding to a novel site in tubulin: A possible mechanistic basis of its antitumor action
35. Suppression of microtubule dynamics by LY290181: A potential mechanism for its antiproliferative action
36. Interaction of the antitumor compound cryptophycin-52 with tubulin
37. The fluorescent probe prodan characterizes the warfarin-binding site on human serum albumin
38. Spectroscopic studies of interaction of coenzymes and coenzyme fragments with pig heart, oxidized triphosphopyridine nucleotide specific isocitrate dehydrogenase
39. Differential binding of the fluorescent probe 8-anilinonaphthalene-2-sulfonic acid to rhodanese catalytic intermediates
40. Interaction of acrylodan with human serum albumin. A fluorescence spectroscopic study
41. Binding of Promen fluorescent probe to human serum albumin. A fluorescence spectroscopic study
42. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
43. Resonance energy transfer: Methods and applications
44. Fluorescence energy transfer as a spectroscopic ruler
45. The use of singlet-singlet energy transfer to study macromolecular assemblies
46. Fluorescence resonance energy transfer studies on the proximity relationship between the intrinsic metal ion and substrate binding sites of Escherichia coli RNA polymerase
47. Unfolding free energy changes determined by linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl α-chymotrypsin using different denaturants
48. Thermodynamic features of thermal unfolding of human serum albumin
49. Crystal structure of human serum albumin at 2.5 Å resolution
50. Crystal structure analysis of warfarin binding to human serum albumin - Anatomy of drug site 1
51. Fatty acid binding to human serum albumin: New insights from crystallographic studies
52. Structure is lost incrementally during the unfolding of barstar
53. Multiple intermediates and transition states during protein unfolding