Secretory expression of human albumin domains in Saccharomyces cerevisiae and their binding of myristic acid and an acylated insulin analogue

Protein Expression and Purification

Volumn 13, Issue 2, 1998, Pages 163-169

  Kjeldsen, Thomas ^a   Pettersson, Annette F ^a   Drube, Lene ^a   Kurtzhals, Peter ^a   Jonassen, Ib ^a   Havelund, Svend ^a   Hansen, Per H ^a   Markussen, Jan ^a  

^a NOVO NORDISK A S   (Denmark)

Author keywords

Fatty acid; Folding; Insulin, prepro leader, pro peptide; Ligand binding; Secretion; Yeast

Indexed keywords

ALBUMIN; EXPRESSION VECTOR; INSULIN; MYRISTIC ACID; PROTEIN EXPRESSION; PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0032127653     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0876     Document Type: Article

References (24)

1. Peters T. P. All about Albumin. 1996;Academic Press, London.
2. Peters T. Serum albumin. Adv. Protein Chem. 37:1985;161-245.
3. Hamilton J. A., Era S., Bhamidipati S. P., Reed R. G. Locations of the three primary binding sites for long-chain fatty acids on bovine albumin. Proc. Natl. Acad. Sci. USA. 88:1991;2051-2054.
4. Ashbrook J. D., Spector A. A., Santos E. C., Fletcher J. E. Long chain fatty acid binding to human plasma albumin. J. Biol. Chem. 250:1975;2333-2338.
5. Reed R. G. Location of long chain fatty acid-binding sites of bovine serum albumin by affinity labeling. J. Biol. Chem. 261:1986;15619-15624.
6. He X. M., Carter D. C. Atomic structure and chemistry of human serum albumin. Nature. 358:1992;209-215.
7. Carter D. C., Ho J. X. Structure of serum albumin. Adv. Protein Chem. 45:1994;153-203.
8. Sleep D., Belfield G. P., Goodey A. R. The secretion of human serum albumin from the yeastSaccharomyces cerevisiae. Bio/Technology. 8:1990;42-46.
9. Okabayashi K., Nakagawa Y., Hayasuke N., Ohi H., Miura M., Ishida Y., Shimizu M., Murakami K., Hirabayashi K., Minamino H., Horii H., Masaki A., Sumi A., Ohmura T., Kawabe H. Secretory expression of the human serum albumin gene in the yeast,Saccharomyces cerevisiae. J. Biochem. 110:1991;103-110.
10. Blondeau K., Boze H., Jung G., Moulin G., Galzy P. Physiological approach to heterologous human serum albumin production byKluyvermyces lactis. Yeast. 10:1994;1297-1303.
11. Fitos I., Visy J., Simoncsits A. Binding studies with recombinant human serum albumin obtained by expression of a synthetic gene in yeast. Biochem. Pharmacology. 46:1993;1159-1163.
12. Ikegaya K., Hirose M., Ohmura T., Nokihara K. Complete determination of disulfide forms of purified recombinant human serum albumin, secreted by the yeastPichia pastoris. Anal. Chem. 69:1997;1986-1991.
13. Fleer R., Yeh P., Amellal N., Maury I., Fournier A., Bacchetta F., Baduel P., Jung G., L'Hote H., Becquart J., Fukuhara H., Mayaux J. F. Stable multicopy vectors for high-level secretion of recombinant human serum albumin byKluyveromyces. Bio/Technology. 9:1991;968-975.
14. Dodsworth N., Harris R., Denton K., Woodrow J., Wood P. C., Qurik A. Comparative studies of recombinant human albumin and human serum albumin derived by blood fractionation. Biotechnol. Appl. Biochem. 24:1996;171-176.
15. Kjeldsen T., Pettersson A. F., Hach M., Diers I., Havelund S., Hansen P. H., Andersen A. S. Synthetic leaders with potential BiP binding mediate high-yield secretion of correctly folded insulin precursors fromSaccharomyces cerevisiae. Protein Expression Purif. 9:1997;331-336.
16. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
17. Thim L., Hansen M. T., Norris K., Hoegh I., Boel E., Forstrom J., Ammerer G., Fiil N. P. Secretion and processing of insulin precursors in yeast. Proc. Natl. Acad. Sci. USA. 83:1986;6766-6770.
18. Kjeldsen T., Brandt J., Andersen A. S., Egel-Mitani M., Hach M., Pettersson A. F., Vad K. A removable spacer peptide in an alpha-factor-leader/insulin precursor fusion protein improves processing and concomitant yield of the insulin precursor inSaccharomyces cerevisiae. Gene. 170:1996;107-112.
19. Markussen J., Havelund S., Kurtzhals P., Andersen A. S., Halstrøm J., Hasselager E., Larsen U. D., Ribel U., Schaffer L., Vad K., Jonassen I. Soluble, fatty acid acylated insulins bind to albumin and show protracted action in pigs. Diabetologia. 39:1996;281-288.
20. Kurtzhals P., Havelund S., Jonassen I., Kiehr B., Larsen U. D., Ribel U., Markussen J. Albumin binding of insulins acylated with fatty acids: Characterization of the ligand-protein interaction and correlation between binding affinity and timing of the insulin effectin vivo. Biochem. J. 312:1995;725-731.
21. Kurtzhals P., Havelund S., Jonassen I., Kiehr B., Ribel U., Markussen J. Albumin binding and time action of acylated insulins in various species. J. Pharmacol. Sci. 85:1996;304-308.
22. (125). Diabetologia. 19:1980;546-554.
23. Reed R. G., Feldhoff R. C., Clute O. L., Peters T. J. Fragments of bovine serum albumin produced by limited proteolysis. Conformation and ligand binding. Biochemistry. 14:1975;4578-4583.
24. Ashbrook J. D., Spector A. A., Fletcher J. E. Medium chain fatty acid binding to human plasma albumin. J. Biol. Chem. 247:1972;7038-7042.