NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii: Cloning, sequence determination and overexpression in Escherichia coli

FEMS Microbiology Letters

Volumn 209, Issue 2, 2002, Pages 155-160

  Camacho, Mónica ^a   Rodríguez Arnedo, Adoración ^a   Bonete, María José ^a  

^a UNIVERSITY OF ALICANTE   (Spain)

Author keywords

Archaea; Haloferax volcanii; Halophile; Isocitrate dehydrogenase

Indexed keywords

AMINO ACID; BUFFER; DNA; EDETIC ACID; ISOCITRATE DEHYDROGENASE; MAGNESIUM CHLORIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SODIUM CHLORIDE; UREA;

ARTICLE; BINDING AFFINITY; CELL INCLUSION; CONTROLLED STUDY; CYTOPLASM; DILUTION; EMBL NUCLEOTIDE SEQUENCE DATABASE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE ISOLATION; GENE LIBRARY; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC CODE; HALOFERAX VOLCANII; HALOPHILIC BACTERIUM; INCUBATION TIME; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ROOM TEMPERATURE; SOLUBILIZATION;

AMINO ACID SEQUENCE; BACTERIOPHAGE LAMBDA; CLONING, MOLECULAR; CONSERVED SEQUENCE; DATABASES, NUCLEIC ACID; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HALOFERAX VOLCANII; ISOCITRATE DEHYDROGENASE; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; PHYLOGENY; POLYMERASE CHAIN REACTION; PROTEIN FOLDING; RECOMBINANT PROTEINS;

ARCHAEA; ESCHERICHIA COLI; HALOFERAX; HALOFERAX VOLCANII; HALOPHILIC ARCHAEON; VECTORS;

EID: 0037046272     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)00469-X     Document Type: Article

References (20)

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