Use of fluorescence polarization detection for the measurement of fluopeptide™ binding to G protein-coupled receptors

Journal of Receptors and Signal Transduction

Volumn 22, Issue 1-4, 2002, Pages 333-343

  Gagne, A ^a   Banks, P ^a   Hurt, Stephen D ^a,b  

^a PERKINELMER INC   (United States)

^b PerkinElmer Life Sciences   (United States)

Author keywords

Binding; Fluorescence; Ligand; Peptide; Receptor

Indexed keywords

BRADYKININ; G PROTEIN COUPLED RECEPTOR; LIGAND; MELANIN CONCENTRATING HORMONE; MELANOCORTIN RECEPTOR; MEMBRANE RECEPTOR; RADIOLIGAND;

BINDING ASSAY; CONFERENCE PAPER; DRUG SCREENING; DRUG TARGETING; FLAME PHOTOMETRY; FLUORESCENCE POLARIZATION; HUMAN; LIGAND BINDING; RADIOASSAY; RECEPTOR AFFINITY; RECEPTOR BINDING;

ALPHA-MSH; BINDING, COMPETITIVE; BORON COMPOUNDS; FLUORESCEINS; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HETEROTRIMERIC GTP-BINDING PROTEINS; HUMANS; LIGANDS; MELANINS; PEPTIDE FRAGMENTS; RADIOLIGAND ASSAY; RECEPTORS, CELL SURFACE; RECEPTORS, CORTICOTROPIN; RECEPTORS, MELANOCORTIN; RECEPTORS, PEPTIDE; SENSITIVITY AND SPECIFICITY;

EID: 0036978077     PISSN: 10799893     EISSN: 15324281     Source Type: Journal    
DOI: 10.1081/RRS-120014605     Document Type: Conference Paper

References (11)

1. Stadel, J.M.; Wilson, S.; Bergsma, D.J. Orphan G Protein-Coupled Receptors: A Neglected Opportunity for Pioneer Drug Discovery. Trends Pharmacol. Sci. 1997, 18 (11), 430-437.
2. Owicki, J.C. Fluorescence Polarization and Anisotropy in High Throughput Screening: Perspectives and Primer. J. Biomol. Screening 2000, 5 (5), 297-306.
3. Allen, M.; Reeves, J.; Mellor, G. High Throughput Fluorescence Polarization: A Homogeneous Alternative to Radioligand Binding for Cell Surface Receptors. J. Biomol. Screening 2000, 5, 63-69.
4. Zhang, J.-H.; Chung, T.D.Y.; Oldenburg, K.R. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J. Biomol. Screening 1999, 4, 67-73.
5. Banks, P.; Gosselin, M.; Prystay, L. Impact of a Red-Shifted Dye Label for High Throughput Fluorescence Polarization Assays of G Protein-Coupled Receptors. J. Biomol. Screening 2000, 5, 329-334.
6. Banks, P.; Gosselin, M.; Prystay, L. Fluorescence Polarization Assays for High Throughput Screening of G Protein-Coupled Receptors. J. Biomol. Screening 2000, 5, 159-167.
7. Fisher, S.L.; Yagaloff, K.A.; Burn, P. Melanocortin and Leptin Signaling Systems: Central Regulation of Catabolic Energy Balance. J. of Recept. Signal Transd. Res. 1999, 19, 203-216.
8. McEachern, A.E.; Shelton, E.R.; Bhakta, S.; Obernolte, R.; Bach, C.; Zuppan, P.; Fujisaki, J.; Aldrich, R.W.; Jarnagin, K. Expression Cloning of a Rat B2 Bradykinin Receptor. Proc. Natl. Acad. Sci. USA 1991, 88, 7724-7728.
9. Hess, J.K.; Borkowski, J.A.; Young, G.S.; Strader, C.D.; Ransom, R.W. Cloning and Pharmacological Characterization of a Human Bradykinin (BK-2) Receptor. Biochem. Biophys. Res. Commun. 1992, 184, 260-268.
10. Hock, F.J.; Wirth, K.; Albus, U.; Linz, W.; Gerhards, H.J.; Weimer, G.; Henke, S.; Breipohl, G.; Konig, W.; Knolle, J. HOE 140 a New and Potent Long Acting Bradykinin Antagonist: In Vitro Studies. Br. J. Pharmacol. 1991, 102, 769-773.
11. Hintermann, E.; Drozdz, R.; Tanner, H.; Eberle, A.N. Synthesis and Characterization of New Radioligands for the Mammalian Melanin-Concentrating Hormone (MCH) Receptor. J. Recept. Signal Transd. Res. 1999, 19, 411-422.