Telomere length analysis on cord blood cells by the flow-FISH method

Haematologia

Volumn 32, Issue 3, 2002, Pages 265-269

  Regéczy, Nóra ^a,b   Valent, Sándor ^d   Kormos, Luca ^c   Hajdu, Melinda ^a   Gopcsa, Laszlo ^a   Pálóczi, Katalin ^a,c  

^a NATIONAL MEDICAL CENTER   (Hungary)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^c SEMMELWEIS UNIVERSITY   (Hungary)

^d National Medical Center ^*

Author keywords

Cord blood; Flow FISH; Telomere length

Indexed keywords

CD19 ANTIGEN; CD3 ANTIGEN; CD34 ANTIGEN;

ALLOGENIC BONE MARROW TRANSPLANTATION; ARTICLE; BLOOD CELL; CELL LINE; CELL SUBPOPULATION; CHROMOSOME SIZE; CONTROLLED STUDY; DISEASE COURSE; DISEASE MARKER; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; MONONUCLEAR CELL; NEWBORN; NORMAL HUMAN; NUCLEIC ACID PROBE; RECIPIENT; STEM CELL; TELOMERE; TELOMERIC REPEAT AMPLIFICATION PROTOCOL; UMBILICAL CORD BLOOD;

BLOOD SPECIMEN COLLECTION; CELL CULTURE TECHNIQUES; FETAL BLOOD; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; METHODS; TELOMERE;

EID: 0036962653     PISSN: 00176559     EISSN: None     Source Type: Journal    
DOI: 10.1163/15685590260461075     Document Type: Article

References (13)

1. Blackburn, E. H., Structure and funcion of telomeres, Nature 530, 569 (1991).
2. Blackburn, E. H., Telomerases, Annu. Rev. Biochem. 61, 113 (1992).
3. Hultdin, M., Grönlund, E., Norrback, K. F., Eriksson-Lindström, E., Just, T., Roos, G., Telomere analysis by fluorescence in situ hybridization and flow cytometry, Nucleic Acids Res. 26, 3651 (1998).
4. Rufer, N., Brümmendorf, T. H., Kolvraa, S., Bischoff, C., Christensen, K., Wadsworth, L., Schulzer, M., Lansdorp, P. M., Telomere fluorescence measurements in granulocytes and T lymphocyte subsets point to a high turnover of hematopoietic stem cells and memory T cells in early childhood, J. Exp. Med. 190, 157 (1999).
5. Rufer, N., Dragowska, W., Thornbury, G., Roosnek, E., Lansdorp, P. M., Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry, Nature Biotechnol. 16, 743 (1998).
6. Notaro, R., Cimmino, A., Tabarini, D., Rotoli, B., Luzzatto, L., In vivo telomere dynamics of human hematopoietic stem cells, Proc. Natl. Acad. Sci. USA 94, 13782 (1997).
7. Brümmendorf, T. H., Holyoake, T. L., Rufer, N., Barnett, M. J., Schulzer, M., Eaves, C. J., Eaves, A. C., Lansdorp, P. M., Prognostic implications of differences in telomere length between normal and malignant cells from patients with chronic myeloid leukaemia measured by flow cytometry, Blood 95, 1883 (2000).
8. Wynn, R. F., Cross, M. A., Hatton, C., Will, A. M., Lashford, L. S., Dexter, T. M., Testa, N. G., Accelerated telomere shortening in young recipients of allogeneic bone-marrow transplants, Lancet 351, 178 (1998).
9. Akiyama, M., Hoshi, Y., Sakurai, H., Yamada, H., Yamada, O., Mizoguchi, H., Changes of telomere length in children after hematopoietic stem cell transplantation, Bone Marrow Transplant. 21, 167 (1998).
10. Vaziri, H., Dragowska, W., Allsopp, R. C., Thomas, T. E., Harley, C. B., Lansdorp, P. M., Evidence for mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age, Proc. Natl. Acad. Sci. USA 91, 9857 (1994).
11. Paloczi, K., Batai, A., Gopcsa, L., Ezsi, R., Petranyi, G. Gy., Immunophenotypic characterisation of cord blood B lymphocytes, Bone Marrow Transplant. 22, 89 (1998).
12. Weng, N. P., Hathcock, K. S., Hodes, R. J., Regulation of telomere length and telomerase in T and B cells: a mechanism for maintaining replicative potential, Immunity 9, 151 (1998).
13. Chatziantoniou, V., Telomerase: biological function and potential role in cancer management, Pathol. Oncol. Res. 7, 161 (2001).