Overexpression and functional characterization of a serine carboxypeptidase inhibitor (IC) from Saccharomyces cerevisiae

Journal of Biochemistry

Volumn 132, Issue 6, 2002, Pages 967-973

  Mima, Joji ^a   Suzuki, Hidekazu ^a   Takahashi, Mieko ^a   Hayashi, Rikimaru ^a  

^a NONE

Author keywords

Carboxypeptidase inhibitor; Carboxypeptidase Y; IC; Overexpression; Proteinase inhibitor complex

Indexed keywords

CARBOXYPEPTIDASE C INHIBITOR; ENZYME INHIBITOR; METHIONINE; SERINE CARBOXYPEPTIDASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; CONTROLLED STUDY; DISULFIDE BOND; DNA VECTOR; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME STRUCTURE; ENZYME SUBSTRATE; FUNGAL STRAIN; GEL FILTRATION CHROMATOGRAPHY; GENE OVEREXPRESSION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SPECTROSCOPY; YEAST;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; VECTORS;

EID: 0036947385     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003311     Document Type: Article

References (36)

1. Turk, V. and Bode, W. (1991) The cystatins: protein inhibitors of cysteine proteinases. FEBS Lett. 285, 213-219
2. Stubbs, M.T., Laber, B., Bode, W., Huber, R., Jerala, R., Lenarcic, B., and Turk, V. (1990) The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction. EMBO J. 9, 1939-1947
3. Martin, J.R., Jerala, R., Kroon-Zitko, L., Zerovnik, E., Turk, V., and Waltho, J.P. (1994) Structural characterization of human stefin A in solution and implications for binding to cysteine proteinases. Eur. J. Biochem. 225, 1181-1194
4. Lenney, J.F. and Dalbec, J.M. (1969) Yeast proteinase B: identification of the inactive form as an enzyme-inhibitor complex. Arch. Biochem. Biophys. 129, 407-409
5. Matern, H., Hoffmann, M., and Holzer, H. (1974) Isolation and characterization of the carboxypeptidase Y inhibitor from yeast. Proc. Natl. Acad. Sci. USA 71, 4874-4878
6. Saheki, T., Matsuda, Y., and Holzer, H. (1974) Purification and characterization of macromolecular inhibitors of proteinase A from yeast. Eur. J. Biochem. 47, 325-332
7. Betz, H., Hinze, H., and Holzer, H. (1974) Isolation and properties of two inhibitors of proteinase B from yeast. J. Biol. Chem. 249, 4515-4521
8. Ulane, R.E. and Cabib, E. (1974) The activating system of chitin synthetase from Saccharomyces cerevisiae Purification and properties of an inhibitor of the activating factor. J Biol. Chem. 249, 3418-3422
9. A, gene. Studies of cytoplasmic proteinase inhibitor deficiency on yeast physiology. FEBS Lett. 283, 78-84
10. Schu, P., Suarez Rendueles, P., and Wilf, D.H. (1991) The proteinase yscB inhibitor (PBI2) gene of yeast and studies on the function of its protein product. Eur. J. Biochem. 197, 1-7
11. 3 inhibitor and its target aspartic proteinase from Saccharomyces cerevisiae. J Biol. Chem. 276, 2023-2030
12. Kojima, S., Deguchi, M., and Miura, K. (1999) Involvement of the C-terminal region of yeast proteinase B inhibitor 2 in its inhibitory action. J. Mol. Biol. 286, 775-785
13. Li, M., Phylip, L.H., Lees, W.E., Winther, J.R., Dunn, B.M., Wlodawer, A., Kay, J., and Gustchina, A. (2000) The aspartic proteinase from Saccharomyces cerevisiae folds its own inhibitor into a helix. Nat. Struct. Biol. 7, 113-117
14. B cooperates with Sec18p (NSF) to promote yeast vacuole inheritance. J. Cell Biol. 136, 299-306
15. B is a small cytosolic protein that participates in vacuole fusion. Proc. Natl. Acad. Sci. USA 94, 5582-5587
16. Matern, H., Barth, R., and Holzer, H. (1979) Chemical and physical properties of the carboxypeptidase Y-inhibitor from baker's yeast. Biochim. Biophys. Acta 567, 503-510
17. Robinson, L.C. and Tatchell, K. (1991) TFS1: A suppressor of cdc25 mutations in Saccharomyces cerevisiae. Mol. Gen. Genet. 230, 241-250
18. Bruun, A.W., Svendsen, I., Sorensen, S.O., Kielland-Brandt, M.C., and Winther, J.R. (1998) A high-affinity inhibitor of yeast carboxypeptidase Y is encoded by TFS1 and shows homology to a family of lipid binding proteins. Biochemistry 37, 3351-3357
19. Schoentgen, F., and Jollès, P. (1995) From structure to function: possible biological roles of a new widespread protein family binding hydrophobic ligands and displaying a nucleotide binding site. FEBS Lett. 369, 22-26
20. Schomerus, C. and Kuntzel, H. (1992) CDC25-dependent induction of inositol 1, 4, 5-trisphosphate and diacrylglycerol in Saccharomyces cerevisiae by nitrogen. FEBS Lett. 307, 249-252
21. Hansen, R.J., Switzer, R.L., Hinze, H., and Holzer, H. (1977) Effects of glucose and nitrogen source on the levels of proteinases, peptidases, and proteinase inhibitors in yeast. Biochim. Biophys. Acta 496, 103-114
22. Tanimizu, N., and Hayashi, R. (1996) Changes in vacuolar protease activities during synchronous culture of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 60, 1526-1527
23. Hayashi, R. (1976) Carboxypeptidase Y. Methods Enzymol. 45, 568-572
24. Maki, T., Kozawa, H., Mima, J., Ueno, H., and Hayashi, R. (2002) Increased proteolytic susceptibility of carboxypeptidase Y caused by modification of the disulfide zipper. Biosci. Biotechnol. Biochem. 66, 1393-1395
25. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., and Klenk, D.C. (1985) Measurement of protein using bicinchonic acid. Anal. Biochem. 150, 76-85.
26. Greenfield, N. and Fasman, G.D. (1969) Computed circular dichroism spectra for the evaluation of protein conformation. Biochemistry 8, 4103-4116
27. Gething, M.J. and Davidson, B.E. (1972) The molar absorption coefficient of reduced Ellman's reagent: 3-carboxylato-4-nitrothiophenolate. Eur. J. Biochem. 30, 352-353
28. Boyer, P.D. (1954) Spectrophotometric study of the reaction of protein sulfhydryl groups with organic mercurials. J. Am. Chem. Soc. 76, 4331-4337
29. Henderson, P.J.F. (1972) A linear equation that describes the steady-state kinetics of enzymes and subcellular particles interacting with tightly bound inhibitors. Biochem. J 127, 321-333
30. Barth, R., Wolf, D.H., and Holzer, H. (1978) Studies on the carboxypeptidase Y-inhibitor complex of yeast. Biochim. Biophys. Acta 527, 63-69
31. Endrizzi, J.A., Breddam, K., and Remington, S.J. (1994) 2.8-Å structure of yeast serine carboxypeptidase. Biochemistry 33, 11106-11120
32. Mima, J., Jung, G., Onizuka, T., Ueno, H., and Hayashi, R. (2002) Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y. Eur. J. Biochem. 269, 3220-3225
33. Boross, L. (1969) Secondary SH-disulfide exchange reactions following the modification of nucleophilic SH-groups in D-glyceraldehyde-3-phosphate dehydrogenase with DTNB. Acta Biochim. Biophys. 4, 57-69
34. Sørensen, S.O. and Winther, J.R. (1994) Active-site residues of procarboxypeptidase Y are accessible to chemical modification. Biochim. Biophys. Acta 1205, 289-293
35. Takeuchi, Y., Satow, Y., Nakamura, K.T., and Mitsui, Y. (1991) Refined crystal structure of the complex of subtilisin BPN′ and Streptomyces subtilisin inhibitor at 1.8 Å resolution. J. Mol. Biol. 221, 309-325
36. Rees, D.C. and Lipscomb, W.N. (1982) Refined crystal structure of the potato inhibitor complex of carboxypeptidase A at 2.5 Å resolution. J. Mol. Biol. 160, 475-498