Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y

European Journal of Biochemistry

Volumn 269, Issue 13, 2002, Pages 3220-3225

  Mima, Joji ^a   Jung, Giman ^a   Onizuka, Takuo ^a   Ueno, Hiroshi ^a   Hayashi, Rikimaru ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Amphipathic property; Carboxypeptidase Y; Substrate binding site; Tetrahedral intermediate; Thiol group

Indexed keywords

AMINO ACID; ASPARTIC ACID; CARBOXYPEPTIDASE C; CYSTEINE; GLYCINE; HISTIDINE; IMIDAZOLE DERIVATIVE; MUTANT PROTEIN; PHENYLALANINE; SERINE; THIOL GROUP; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CATALYSIS; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME SUBSTRATE; HYDROPHOBICITY; MICHAELIS CONSTANT; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS;

CARBOXYPEPTIDASE C; CARBOXYPEPTIDASES; CATALYTIC DOMAIN; HISTIDINE; KINETICS; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; STRUCTURE-ACTIVITY RELATIONSHIP; SULFHYDRYL COMPOUNDS; VALINE;

EID: 0036301644     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02997.x     Document Type: Article

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