Increased proteolytic susceptibility of carboxypeptidase y caused by modification of the disulfide zipper

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 6, 2002, Pages 1393-1395

  Maki, Toshihiko ^a   Kozawa, Hajime ^a   Mima, Joji ^a   Ueno, Hiroshi ^a,b   Hayashi, Rikimaru ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NARA WOMEN'S UNIVERSITY   (Japan)

Author keywords

Carboxypeptidase Y; Disulfide zipper; Pro carboxypeptidase Y; Protease susceptibility; Structural motif

Indexed keywords

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

CARBOXYPEPTIDASE; CARBOXYPEPTIDASE C; DISULFIDE; PROTEINASE;

ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE; WESTERN BLOTTING;

BLOTTING, WESTERN; CARBOXYPEPTIDASE C; CARBOXYPEPTIDASES; DISULFIDES; ENDOPEPTIDASES; ENZYME STABILITY; MUTATION; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE;

EID: 0036616666     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.1393     Document Type: Article

References (8)

1. Hayashi, R., Bai, Y., and Hata, T., Kinetic studies of carboxypeptidase Y. I. Kinetic parameters for the hydrolysis of synthetic substrates. J. Biochem., 77, 69-79 (1975).
2. Hayashi, R. and Hata, T., Action of yeast proteinase C on synthetic peptides and poly-, amino acids. Biochem. Biophys. Acta, 263, 673-679 (1972).
3. Endrizzi, J. A., Breddam, K., and Remington, S.J., 2.8-Â structure of yeast serine carboxypeptidase. Biochemistry, 33, 11106-11120 (1994).
4. Liao, D. -I., Breddam, K., Sweet, R. M., Bullock, T., and Remington, S. J., Refined atomic model of wheat serine carboxypeptidase II at 2.2-Â resolution. Biochemistry, 31, 9796-9812 (1992).
5. Rudenko, G., Bonten, E., d’Azzo, A., and Hol, W. G., Three-dimensional structure of the human ‘protective protein’: structure of the precursor form suggests a complex activation mechanism. Structure, 15, 1249-1259 (1995).
6. Matsuzaki, H., Ueno, H., Hayashi, R., and Liao, T. H., Bovine spleen cathepsin A: characterization and comparison with the protective protein. J. Biochem., 123, 701-706 (1998).
7. Hayashi, R., Bai, Y., and Hata, T., Further confirmation of carboxypeptidase Y as a metal-free enzyme having a reactive serine residue. J. Biochem., 77, 1313-1318 (1975).
8. Wolf, D. H., Proteinase action in vitro versus proteinase function in vivo: mutants shed light on intracellular proteolysis in yeast. TIBS, 7, 35-37 (1982).