Cloning of an antifreeze protein gene from carrot and its influence on cold tolerance in transgenic tobacco plants

Plant Cell Reports

Volumn 21, Issue 4, 2002, Pages 296-301

  Fan, Y ^a   Liu, B ^a   Wang, H ^a   Wang, S ^a   Wang, J ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Antifreeze protein gene; Carrot; Cold induced; Ion leakage assay; Transformation

Indexed keywords

DNA; GENES; PROTEINS; SEED; TOBACCO;

CARROT ANTIFREEZE PROTEIN (AFT); POLYMERASE CHAIN REACTION (PCR);

CLONING;

DAUCUS CAROTA; EMBRYOPHYTA; ESCHERICHIA COLI; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 0036923208     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-002-0495-3     Document Type: Article

References (15)

1. Clark MS, Gu H-Y, Qu L-J (1998) Plant Molecular Biology a Laboratory Manual (Chinese translation). Chinese High Education Press, Beijing
2. Cui Q (1995) Plant Physiology and Biochemistry - A Laboratory Manual. Chinese Agriculture Press, Beijing
3. DeVries AL, Komatsu SK, Feeney RE (1970) Chemical and physical properties of freezing point-depressing glycoproteins from Antarctic fishes. J Biol Chem 245:2901-2908
4. Dityatkin SY, Lisovskaya KV, Panzhava NN, Iliashenko BN (1972) Frozen-thawed bacteria as recipients of isolated coliphage DNA. Biochem Biophys Acta 281:319-323
5. Holsters M, de Waele D, Depicker A, Messens E, van Montagu M, Schell J (1978) Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163:181-187
6. Horsch RB, Fry J, Hofmann N, Neidermeyer J, Rogers SG, Fraley RT (1998) Leaf disc transformation. In: Gelvin SB, Schilperoot RA (eds) Plant Molecular Biology Manual. Kluwer, Dordrecht, pp A5/1-A5/9
7. Jang Y, Jia S-R, Fei Y-B, Tan K-H (1999) Antifreeze proteins and their role in plant antifreeze physiology (in Chinese). Acta Bot Sin 41:677-685
8. Meyer K, Keil M, Naldrett MJ (1999) A leucine-rich repeat protein of carrot that exhibits antifreeze activity. FEBS Lett 447:171-178
9. Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13:7207-7221
10. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491
11. Sambrook J, Fritsch EF, Maniatis JT, Jin D-Y, Li M-F, et al. (1993) Molecular Cloning - A Laboratory Manual, 2nd Edn. (Chinese translation). Science Press, Beijing
12. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503-517
13. Wallis JG, Wang H, Guerra DJ (1997) Expression of a synthetic antifreeze protein in potato reduces electrolyte release at freezing temperatures. Plant Mol Biol 35:323-330
14. Worrall D, Elias L, Ashford D, Smallwood M, Sidebottom C, Lillford P, Telford J, Holt C, Bowles D (1998) A carrot leucine-rich-repeat protein that inhibits ice recrystallization. Science 282:115-117
15. Wu D-Z (1984) Introduce a fine variety of flue-cured tobacco NC82 (in Chinese). Chin Tobacco 1:20-21