Quantitative detection of Escherichia coli 0157:H7 in ground beef by immunomagnetic separation and competitive polymerase chain reaction

Food Biotechnology

Volumn 16, Issue 3, 2002, Pages 155-166

  Guan, J ^a   Levin, R E ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Beef; E. coli 0157:H7; PCR; Quantitative; SLT 1; SLT 2

Indexed keywords

CONCENTRATION (PROCESS); DNA; ESCHERICHIA COLI; IMMUNOLOGY;

GROUND BEEF; IMMUNOMAGNETIC SEPARATION (IMS);

BIOTECHNOLOGY;

ESCHERICHIA COLI;

EID: 0036914598     PISSN: 08905436     EISSN: None     Source Type: Journal    
DOI: 10.1081/FBT-120016665     Document Type: Article

References (21)

1. Patton, J.C.; Paton, A.W. Pathogenesis and diagnosis of shiga toxin-producing escherichia coli infections. Clin. Microbiol. Rev. 1998, 11, 450-479.
2. Shelton, D.R.; Karns, J. Quantitative detection of Escherichia coli 0157:H7 in surface waters by using Immunomagnetic Electrochemiluminescence. Appl. Environ. Microbiol. 2001, 67, 2908-2915.
3. Elder, R.O.; Keen, J.E.; Siragusa, G.R.; Barkocy-Gallapher, G.A.; Koohmaraie, M.; Laegreid, W.W. Correlation of enterohemorrhagic escherichia coli 0157:H7 prevalence in feces, hides, land carcasses of beef cattle during processing. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 2999-3003.
4. Centers for Disease Control. Surveillance for foodborne disease outbreaks - United States, 1993-1997. Morbidity and Mortality Weekly Report. 2000, Vol. 49, No. SS-1.
5. Gooding, C.M.; Choudary, P.V. Detection of Escherichia coli 0157:H7 in ground beef in eight hours. J. Microbial. Methods. 1998, 34, 89-98.
6. Gilgen, M.; Hübner, P.; Höfelin, C.; Lüthy, J.; Candrian, U. PCR-based detection of verotoxin-producing Escherichia coli (VTEC) in ground beef. Res. Microbiol. 1998, 149, 145-54.
7. Chen, J.; Honson, R.; Griffiths, M. Detection of verotoxigenic Escherichia coli by magnetic capture-hybridization PCR. Appl. Environ. Microbiol. 1998, 64, 147-152.
8. Forti, N.Y.; Mulchandani, A.; Chen, W. Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli 0157:H7. Anal. Biochem. 2001, 289, 281-288.
9. Oberst, R.D.; Hays, M.P.; Hoha, L.K.; Phebus, R.K.; Yamashiro, C.T.; Paszko-Kolva, C.; Flood, S.J.A.; Sargeant, J.M.; Gillespie, J.R. PCR-based DNA amplification and presumptive detection of Escherichia coli 0157:h7 with an internal fluorogenic probe and the 5′ nuclease (Taq Man) assay. Appl. Environ. Microbiol. 1998, 64, 3389-3396.
10. Witham, P.K.; Yamashiro, C.T.; Livak, K.J.; Batt, C.A. A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beef. Appl. Environ. Microbiol. 1996, 62, 1347-1353.
11. Li, W.; Drake, M.A. Development of a quantitative competitive PCR assay for detection and quantification of E. coli 0157:H7 cells. Appl. Environ. Microbiol. 2001, 67, 3291-3294.
12. Zarchar, V.L.; Thomas, R.A.; Goustin, A.S. Absolute quantification of target DNA: A simple competitive PCR for efficient analysis of multiple samples. Nucleic Acid Res. 1993, 8, 2017-2018.
13. Reilly, K.; Attwook, G.T. Detection of Clostridium proteoclasticum and closely related strains in the rumen by competitive PCR. Appl. Environ. Microbiol. 1998, 64, 907-913.
14. Abolmaaty, A.; Vu, C.; Oliver, J.; Levin, R.E. Development of a new lysis solution for releasing genomic DNA from bacterial cells for DNA amplification by polymerase chain reaction. Microbios. 2000, 101, 181-189.
15. Calderwood, S.B.; Auclair, F.; Donohe-Rolf, A.; Keusch, G.T.; Mekalanos, J.J. Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 1987, 84, 4364-4368.
16. Jackson, M.P.; Neill, R.J.; O'Brien, A.D.; Holmes, R.K.; Newland, J.W. Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933. FEMS Microbiol. Letters. 1987, 44, 109-114.
17. Rupf, S.; Merte, K.; Eschrich, K. Quantification of bacteria in oral samples by competitive polymerase chain reaction. J. Dental Res. 1999, 78, 850-856.
18. Abolmatty, A.; El-Shemy, M.G.; Khallaf, M.F.; Levin, R.E. Effect of lysing methods and their variables on the yield of Escherichia coli 0157:H7 DNA and its PCR amplification. J. Microbial. Methods. 1998, 34, 133-141.
19. Piatek, M.; Luk, K.C.; Williams, B.K.; Lifson, J.D. Quantitative competitive polymerase chain reaction for accurate quantitation of HIV DNA and RNA species. Bio Techniques. 1993, 14, 70-80.
20. Tuttle, J.; Gomez, T.; Doyle, M.P.; Wells, J.G.; Zhao, T.; Tauxe, R.V.; Griffin, P.M. Lessons from a large outbreak of Escherichia coli 0157:H7 infections: Insights into the infectious dose and method of widespread contamination of hamburger patties. Epidomiol. Infect. 1999, 22, 185-192.
21. Venozy-Rozand, C.; Mazuy, C.; Ray-Gueniot, S.; Boutrand-Loei, S.; Meyrand, A.; Richard, Y. Detection of Escherichia coli 0157 in French food samples using an immunomagnetic separation method and the VIDAS™ E. coli 0157. Lett. Appl. Microbial. 1997, 25, 442-446.