Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells

Prenatal Diagnosis

Volumn 22, Issue 12, 2002, Pages 1130-1134

  Rice, John E ^a   Sanchez, J Aquiles ^a   Pierce, Kenneth E ^a   Wangh, Lawrence J ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

Molecular beacons; Preimplantation genetic diagnosis; Real time PCR; Single cell PCR; Tay Sachs disease

Indexed keywords

FLUOROCHROME;

ALLELE; AMPLICON; ARTICLE; ASSAY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENE AMPLIFICATION; GENETIC ANALYSIS; GENOTYPE; HETEROZYGOSITY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; KINETICS; LYMPHOBLAST; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; PRENATAL DIAGNOSIS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; TAY SACHS DISEASE; VALIDATION PROCESS; ADULT; BIOASSAY; CELL LINE; CHEMISTRY; CYTOLOGY; DNA PROBE; FEMALE; GENETICS; LYMPHOCYTE; METHODOLOGY; PREGNANCY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADULT; ALLELES; BIOLOGICAL ASSAY; CELL LINE; DNA PROBES; FEMALE; GENOTYPE; HUMANS; LYMPHOCYTES; PREGNANCY; PREIMPLANTATION DIAGNOSIS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TAY-SACHS DISEASE;

EID: 0036898367     PISSN: 01973851     EISSN: None     Source Type: Journal    
DOI: 10.1002/pd.500     Document Type: Article

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