Polymerase chain reaction amplification specificity: Incidence of allele dropout using different DNA preparation methods for heterozygous single cells

Journal of Assisted Reproduction and Genetics

Volumn 13, Issue 2, 1996, Pages 107-111

  Gitlin, Susan A ^a,b   Lanzendorf, Susan E ^a   Gibbons, William E ^a  

^a EASTERN VIRGINIA MEDICAL SCHOOL   (United States)

^b The Jones Institute for Reproductive Medicine   (United States)

Author keywords

allele dropout; amplification specificity; DNA preparation; polymerase chain reaction; preimplantation genetic diagnosis

Indexed keywords

DITHIOTHREITOL; DNA; POTASSIUM HYDROXIDE;

ALLELE; ARTICLE; DIAGNOSTIC ACCURACY; EMBRYO; EMBRYO TRANSFER; FERTILIZATION IN VITRO; GENE AMPLIFICATION; GENE TARGETING; GENETIC DISORDER; HUMAN; HUMAN CELL; LYMPHOBLAST; POLYMERASE CHAIN REACTION; PREIMPLANTATION EMBRYO; PRENATAL DIAGNOSIS; PRIORITY JOURNAL;

ALLELES; ARTIFACTS; BETA-N-ACETYLHEXOSAMINIDASE; CELL FRACTIONATION; CELLS, CULTURED; DITHIOTHREITOL; DNA; DNA MUTATIONAL ANALYSIS; EXONS; FEASIBILITY STUDIES; FREEZING; HEAT; HETEROZYGOTE; HUMANS; HYDROXIDES; ISOENZYMES; LYMPHOCYTES; MICROCHEMISTRY; NITROGEN; POLYMERASE CHAIN REACTION; POTASSIUM COMPOUNDS; PREDICTIVE VALUE OF TESTS; PRENATAL DIAGNOSIS; SENSITIVITY AND SPECIFICITY; TAY-SACHS DISEASE; WATER;

EID: 0030039944     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02072530     Document Type: Article

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