Biochemical properties of Neisseria gonorrhoeae LgtE

Journal of Bacteriology

Volumn 184, Issue 23, 2002, Pages 6410-6416

  Piekarowicz, Andrzej ^a   Stein, Daniel C ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; GALACTOSE; LIPOOLIGOSACCHARIDE; MANGANESE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE;

ARTICLE; BACTERIAL GENE; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE AMPLIFICATION; LGTE GENE; MOLECULAR CLONING; NEISSERIA GONORRHOEAE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIAL PROTEINS; CARBOHYDRATE SEQUENCE; CULTURE MEDIA; ESCHERICHIA COLI; GALACTOSE; GALACTOSYLTRANSFERASES; GLYCOSYLTRANSFERASES; LIPOPOLYSACCHARIDES; MOLECULAR SEQUENCE DATA; NEISSERIA GONORRHOEAE; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; NEISSERIA; NEISSERIA GONORRHOEAE; VECTORS;

EID: 0036889010     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.23.6410-6416.2002     Document Type: Article

References (37)

1. Arking, D., Y. Tong, and D. C. Stein. 2001. Analysis of lipooligosaccharide biosynthesis in the Neisseriaceae. J. Bacteriol. 183:934-941.
2. Banerjee, A., R. Wang, Uljohn, S., P. A. Rice, E. C. Gotschlich, and D. C. Stein. 1998. Identification of the gene (lgtg) encoding the lipooligosaccharide β chain synthesizing glucosyl transferase from Neisseria gonorrhoeae. Proc. Natl. Acad. Sci. USA 95:10872-10877.
3. Blixt, O., I. van Die, T. Norberg, and D. H. van den Eijnden. 1999. High-level expression of the Neisseria meningitidis lgtA gene in Escherichia coli and characterization of the encoded N-acetylglucosaminyltransferase as a useful catalyst in the synthesis of GIcNAc β1→3Gal and GaINAc β1→3Gal linkages. Glycobiology 9:1061-1071.
4. Burch, C. L., R. J. Danaher, and D. C. Stein. 1997. Antigenic variation in Neisseria gonorrhoeae: Production of multiple lipooligosaccharides. J. Bacteriol. 179:982-986.
5. Danaher, R. J., J. C. Levin, D. Arking, C. L. Burch, R. Sandlin, and D. C. Stein. 1995. Genetic basis of Neisseria gonorrhoeae lipooligosaccharide antigenic variation. J. Bacteriol. 177:7275-7279.
6. Erwin, A. L., P. A. Haynes, P. A. Rice, and E. C. Gotschlich. 1996. Conservation of the lipooligosaccharide synthesis locus lgt among strains of Neisseria gonorrhoeae: Requirement for lgtE in synthesis of the 2C7 epitope and of the beta chain of strain 15253. J. Exp. Med. 184:1233-1241.
7. Fermer, C., B. E. Kristiansen, O. Skold, and G. Swedberg. 1995. Sulfonamide resistance in Neisseria meningitidis as defined by site-directed mutagenesis could have its origin in other species. J. Bacteriol. 177:4669-4675.
8. Frangipane, J. V., and R. F. Rest. 1993. Anaerobic growth and cytidine 5′-monophospho-N-acetylneuraminic acid act synergistically to induce highlevel serum resistance in Neisseria gonorrhoeae. Infect. Immun 61:1657-1666.
9. Gibson, B. W., W. Melaugh, N. J. Phillips, M. A. Apicella, A. A. Campagnari, and J. M. Griffiss. 1993. Investigation of the structural heterogeneity of lipooligosaccharides from pathogenic Haemophilus and Neisseria species and of R-type lipopolysaccharides from Salmonella typhimurium by electrospray mass spectrometry. J. Bacteriol. 175:2702-2712.
10. Gibson, B. W., J. W. Webb, R. Yamasaki, S. J. Fisher, A. L. Burlingame, R. E. Mandrell, H. Schneider, and J. M. Griffiss. 1989. Structure and heterogeneity of the oligosaccharides from the lipopolysaccharides of a pyocinresistant Neisseria gonorrhoeae. Proc. Natl. Acad. Sci. USA 86:17-21.
11. Gotschlich, E. C. 1994. Genetic locus for the biosynthesis of the variable portion of Neisseria gonorrhoeae lipooligosaccharide. J. Exp. Med. 180:2181-2190.
12. Griffiss, J. M., H. Schneider, R. E. Mandrell, R. Yamasaki, G. A. Jarvis, J. J. Kim, B. W. Gibson, R. Hamadeh, and M. A. Apicella. 1988. Lipooligosaccharides: The principal glycolipids of the neisserial outer membrane. Rev. Infect. Dis. 10:S287-295.
13. Jennings, M. P., D. W. Hood, I. R. Peak, M. Virji, and E. R. Moxon. 1995. Molecular analysis of a locus for the biosynthesis and phase-variable expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis. Mol. Microbiol. 18:729-740.
14. Jennings, M. P., Y. N. Srikhanta, E. R. Moxon, M. Kramer, J. T. Poolman, B. Kuipers, and P. van der Ley. 1999. The genetic basis of the phase variation repertoire of lipopolysaccharide immunotypes in Neisseria meningitidis. Microbiology 145:3013-3021.
15. John, C. M., J. M. Griffiss, M. A. Apicella, R. E. Mandrell, and B. W. Gibson. 1991. The structural basis for pyocin resistance in Neisseria gonorrhoeae lipooligosaccharides. J. Biol. Chem. 266:19303-19311.
16. Jones, D. M., R. Borrow, A. J. Fox, S. Gray, K. A. Cartwright, and J. T. Poolman. 1992. The lipooligosaccharide immunotype as a virulence determinant in Neisseria meningitidis. Microb. Pathog. 13:219-224.
17. Kahler, C. M., R. W. Carlson, M. M. Rahman, L. E. Martin, and D. S. Stephens. 1996. Two glycosyltransferase genes, lgtF and rfaK, constitute the lipooligosaccharide ice (inner core extension) biosynthesis operon of Neisseria meningitidis. J. Bacteriol. 178:6677-6684.
18. Kerwood, D. E., H. Schneider, and R. Yamasaki. 1992. Structural analysis of lipooligosaccharide produced by Neisseria gonorrhoeae, strain MS11mk (variant A): A precursor for a gonococcal lipooligosaccharide associated with virulence. Biochemistry 31:12760-12768.
19. McGee, D. J., and R. F. Rest. 1996. Regulation of gonococcal sialyltransferase, lipooligosaccharide, and serum resistance by glucose, pyruvate, and lactate. Infect. Immun. 64:4630-4637.
20. Morse, S. A., C. S. Mintz, S. K. Sarafian, L. Bartenstein, M. Bertram, and M. A. Apicella. 1983. Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture. Infect. Immun 41:74-82.
21. Persson, K., H. D. Ly, M. Dieckelmann, W. W. Wakarchuk, S. G. Withers, and N. C. Strynadka. 2001. Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs. Nat. Struct. Biol. 8:166-175.
22. Pettit, R. K., E. S. Martin, S. M. Wagner, and V. J. Bertolino. 1995. Phenotypic modulation of gonococcal lipooligosaccharide in acidic and alkaline culture. Infect. Immun. 63:2773-2775.
23. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Sandlin, R., and D. C. Stein. 1991. Structural heterogeneity of lipopolysaccharides the Neisseriaceae. FEMS Microbiol. Lett. 90:69-72.
25. c. Infect. Immun. 61:3360-3368.
26. Song, W., L. Ma, W. Chen, and D. C. Stein. 2000. Role of lipooligosaccharide in opa-independent invasion of Neisseria gonorrhoeae into human epithelial cells. J. Exp. Med. 191:949-959.
27. Tong, Y., D. Arking, S. Ye, B. Reinhold, V. Reinhold, and D. C. Stein. 2002. Neisseria gonorrhoeae strain PID2 simultaneously expresses six chemically related lipooligosaccharide structures. Glycobiology 12:523-533.
28. Tong, Y., B. Reinhold, V. Reinhold, B. Brandt, and D. C. Stein, 2001. Structural and immunochemical characterization of the lipooligosaccharides expressed by Neisseria subflava 44. J. Bacteriol. 183:942-950.
29. Tsai, C. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipooligosaccharide in polyacrylamide gels. Anal. Biochem. 119:115-119.
30. Wakarchuk, W., A. Martin, M. P. Jennings, E. R. Moxon, and J. C. Richards. 1996. Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis. J. Biol. Chem. 271:19166-19173.
31. Wakarchuk, W. W., A. Cunningham, D. C. Watson, and N. M. Young. 1998. Role of paired basic residues in the expression of active recombinant galactosyltransferases from the bacterial pathogen Neisseria meningitidis. Protein Eng 11:295-302.
32. Westphal, O., and K. Jann. 1972. Bacterial Lipopolysaccharides: Extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem. 5:83-91.
33. White, L. A., and D. S. Kellogg, Jr. 1965. Neisseria gonorrhoeae identification in direct smears by a fluorescent antibody counterstain method. Appl. Microbiol. 13:171-174.
34. Yamasaki, R., B. E. Bacon, W. Nasholds, H. Schneider, and J. M. Griffiss. 1991. Structural determination of oligosaccharides derived from lipooligosaccharide of Neisseria gonorrhoeae F62 by chemical, enzymatic, and two-dimensional NMR methods. Biochemistry 30:10566-10575.
35. Yamasaki, R., D. E. Kerwood, H. Schneider, K. P. Quinn, J. M. Griffiss, and R. E. Mandrell. 1994. The structure of lipooligosaccharide produced by Neisseria gonorrhoeae, strain 15253, isolated from a patient with disseminated infection. Evidence for a new glycosylation pathway of the gonococcal lipooligosaccharide. J. Biol. Chem. 269:30345-30351.
36. Yamasaki, R., W. Nasholds, H. Schneider, and M. A. Apicella. 1991. Epitope expression and partial structural characterization of F62 lipooligosaccharide (LOS) of Neisseria gonorrhoeae: IgM monoclonal antibodies (3F11 and 1-1-M) recognize nonreducing termini of the LOS components. Mol. Immunol. 28:1233-1242.
37. Yang, Q. L., and E. C. Gotschlich. 1996. Variation of gonococcal lipooligosaccharide structures is due to alterations in poly-G tracts in lgt genes encoding glycosyl transferases. J. Exp. Med. 183:323-327.