Conservation of the lipooligosaccharide synthesis locus lgt among strains of Neisseria gonorrhoeae: Requirement for lgtE in synthesis of the 2C7 epitope and of the β chain of strain 15253

Journal of Experimental Medicine

Volumn 184, Issue 4, 1996, Pages 1233-1241

  Erwin, Alice L ^a   Haynes, Paul A ^a   Rice, Peter A ^b   Gotschlich, Emil C ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; GALACTOSYLTRANSFERASE; GLYCOSYLTRANSFERASE; OLIGOSACCHARIDE;

ARTICLE; BETA CHAIN; CARBOHYDRATE ANALYSIS; CARBOHYDRATE SYNTHESIS; ENZYME ACTIVITY; GENE DELETION; GENE EXPRESSION; GENE LOCUS; GENE TRANSFER; NEISSERIA GONORRHOEAE; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

BLOTTING, SOUTHERN; CARBOHYDRATE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; EPITOPES; GENES, BACTERIAL; GLYCOSYLTRANSFERASES; IMMUNOBLOTTING; LECTINS; LIPOPOLYSACCHARIDES; MOLECULAR SEQUENCE DATA; MONOSACCHARIDES; MUTATION; NEISSERIA GONORRHOEAE; PLANT LECTINS; RESTRICTION MAPPING; SPECIES SPECIFICITY; VARIATION (GENETICS);

EID: 0029961892     PISSN: 00221007     EISSN: None     Source Type: Journal    
DOI: 10.1084/jem.184.4.1233     Document Type: Article

References (38)

1. 2 chemotype LPS and do not enter epithelial host cells. Mol. Microbiol. 15:267-275.
2. Zhou, D., N.-G. Lee, and M.A. Apicella. 1994. Lipooligosaccharide biosynthesis in Neisseria gonorrhoeae: cloning, identification and characterization of the α1,5 heptosyltransferase I gene (rfaC). Mol. Microbiol. 14:609-618.
3. Kahler, C.M., R.W. Carlson, M.M. Rahman, L.E. Martin, and D.S. Stephens. 1996. Inner core biosynthesis of lipooligosaccharide (LOS) in Neisseria meningitidis serogroup B: identification and role in LOS assembly of the α1,2 N-acetylglucosamine transferase (rfaK). J. Bacteriol. 178:1265-1273.
4. van der Ley, P., M. Kramer, L. Steeghs, B. Kuipers, S.R. Andersen, M.P. Jenning, E.R. Moxon, and J.T. Poolman. 1996. Identification of a locus involved in meningococcal lipopolysaccharide biosynthesis by deletion mutagenesis. Mol. Microbiol. 19:1117-1125.
5. Yamasaki, R., D.E. Kerwood, H. Schneider, K.P. Quinn, J.M. Griffiss, and R.E. Mandrell. 1994. The structure of lipooligosaccharide produced by Neisseria gonorrhoeae, strain 15253, isolated from a patient with disseminated infection: evidence for a new glycosylation pathway of the gonococcal lipooligosaccharide. J. Biol. Chem. 269:30345-30351.
6. Mandrell, R., M. Apicella, J. Boslego, R. Chung, P. Rice, and J.M. Griffiss. 1988. Human immune response to monoclonal antibody-defined epitopes of Neisseria gonorrhoeae lipooligosaccharides. In Gonococci and Meningococci: Epidemiology, Genetics, Immunochemistry, and Pathogenesis. J.T. Poolman, H.C. Zanen, T.F. Meyer, J.E. Heckels, P.R.H. Mäkelä, H. Smith, and E.C. Beuvery, editors. Kluwer Academic Publishers, Dordrecht, The Netherlands. 569-574.
7. Gotschlich, E.C. 1994. Genetic locus for the biosynthesis of the variable portion of Neisseria gonorrhoeae LOS. J. Exp. Med. 180:2181-2190.
8. Yang, Q.-L., and E.C. Gotschlich. 1996. Variation of gonococcal lipooligosaccharide structure is due to alterations in poly-G tracts in lgt genes encoding glycosyl transferases. J. Exp. Med. 183:323-327.
9. Danaher, R.J., J.C. Levin, D. Arking, C.L. Burch, R. Sandlin, and D.C. Stein. 1995. Genetic basis of Neisseria gonorrhoeae lipooligosaccharide antigenic variation. J. Bacteriol. 177: 7275-7279.
10. Jennings, M.P., D.W. Hood, I.R.A. Peak, M. Virji, and E.R. Moxon. 1995. Molecular analysis of a locus for the biosynthesis and phase-variable expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis. Mol. Microbiol. 18:729-740.
11. Erwin, A.L., and E.C. Gotschlich. 1996. Cloning of a Neisseria meningitidis gene for L-lactate dehydrogenase; evidence for a second meningococcal L-LDH with different regulation. J. Bacteriol. 178:4807-4813.
12. Drazek, E.S., D.C. Stein, and C.D. Deal. 1995. A mutation in the Neisseria gonorrhoeae rfaD homolog results in altered lipooligosaccharide expression. J. Bacteriol. 177:2321-2327.
13. Bartolomé, B., Y. Jubete, E. Martínez, and F. De la Cruz. 1991. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene (Amst.). 102:75-78.
14. Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. In Methods in Carbohydrate Chemistry. R.C. Whistler and M.L. Wolfrom, editors. Academic Press, New York. 83-91.
15. Lese, A.J., A.A. Campagnari, W.E. Bittner, and M.A. Apicella. 1990. Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Immunol. Methods. 126: 109-117.
16. Tsai, C.-M., and C.E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
17. Towbin, H., T. Staehlin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:4350.
18. Mandrell, R., H. Schneider, M. Apicella, W. Zollinger, P.A. Rice, and J.M. Griffiss. 1986. Antigenic and physical diversity of Neisseria gonorrhoeae lipooligosaccharides. Infect. Immun. 54: 63-49.
19. McQuillen, D.P., S. Gulati, Y.-K. Tsung, J. Sharon, and P.A. Rice. 1994. Experimental immunization with gonococcal anti-idiotype vaccine against gonococcal lipooligosaccharide. In Pathobiology and Immunobiology of Neisseriaceae. C.J. Conde-Glez, S. Morse, P. Rice, F. Sparling, and E. Calderón, editors. Instituto Nacional de Salud Pública, Cuernavaca, Mexico. 867-873.
20. Blake, M.S., K.H. Johnston, G.J. Russell-Jones, and E.C. Gotschlich. 1984. A rapid sensitive method for detection of alkaline phosphatase conjugated anti-antibodies on Western blots. Anal. Biochem. 136:175-179.
21. Hardy, M.R., R.R. Townsend, and Y.C. Lee. 1988. Monosaccharide analysis of glycoconjugates by anion exchange chromatography with pulsed amperometric detection. Anal. Biochem. 170:54-62.
22. Hardy, M. R. 1989. Monosaccharide analysis of glycoconjugates by high-performance anion-exchange chromatography with pulsed amperometric detection. Methods Enzymol. 179: 76-82.
23. Kerwood, D.E., H. Schneider, and R. Yamasaki. 1992. Structural analysis of lipooligosaccharide produced by Neisseria gonorrhoeae, strain MS11mk (variant A): a precursor for a gonococcal lipooligosaccharide associated with virulence. Biochemistry. 31:12760-12768.
24. Schneider, H., J.M. Griffiss, J.W. Boslego, P.J. Hitchcock, K.M. Zahos, and M.A. Apicella. 1991. Expression of paragloboside-like lipooligosaccharides may be a necessary component of gonococcal pathogenesis in men. J. Exp. Med. 174: 1601-1605.
25. Phillips, N.J., M.A. Apicella, J.M. Griffiss, and B.W. Gibson. 1992. Structural characterization of the cell surface lipooligosaccharides from a nontypable strain of Haemophilus influenzae. Biochemistry. 31:4515-4526.
26. Yamasaki, R., B.E. Bacon, W. Nasholds, H. Schneider, and J.M. Griffiss. 1991. Structural determination of oligosaccharides derived from lipooligosaccharide of Neisseria gonorrhoeae F62 by chemical, enzymatic, and two-dimensional NMR methods. Biochemistry. 30:10566-10575.
27. John, C.M., J.M. Griffiss, M.A. Apicella, R.E. Mandrell, and B.W. Gibson. 1991. The structural basis for pyocin resistance in Neisseria gonorrhoeae lipooligosaccharides. J. Biol. Chem. 266:19303-19311.
28. O'Brien, J.P., D.L. Goldenberg, and P.A. Rice. 1983. Disseminated gonococcal infection: a prospective analysis of 49 patients and a review of pathophysiology and immune mechanisms. Medicine. 62:395-406.
29. Rick, P.D., L.W.-M. Fung, C. Ho, and M.J. Osborn. 1977. Lipid A mutants of Salmonella typhimurium: purification and characterization of a lipid A precursor produced by a mutant in 3-deoxy-D-mannooctulosonate-8-phosphate synthetase. J. Biol. Chem. 252:4904-4912.
30. Mohan, S., and C.R.H. Raetz. 1994. Endotoxin biosynthesis in Pseudomonas aeruginosa: enzymatic incorporation of laurate before 3-deoxy-D-manno-octulosonate. J. Bacteriol. 176:6944-6951.
31. Patrick, C.C., G.T. Furuta, M. Edwards, M. Estabrook, M.S. Blake, and C.J. Baker. 1993. Variation in phenotypic expression of the Opa outer membrane protein and lipooligosaccharide of Neisseria meningitidis serogroup C causing periorbital cellulitis and bacteremia. Clin. Infect. Dis. 16:523-527.
32. Mackinnon, F.G., R. Borrow, A.R. Gorringe, A.J. Fox, D.M. Jones, and A. Robinson. 1993. Demonstration of lipooligosaccharide immunotype and capsule as virulence factors for Neisseria meningitidis using an infant mouse intranasal infection model. Microbial Pathogen. 15:359-366.
33. Rice, P.A. 1989. Molecular basis for serum resistance in Neisseria gonorrhoeae. Clin. Microbiol. Rev. 2:S112-S117.
34. van Putten, J.P.M. 1993. Phase variation of lipopolysaccharide directs interconversion of invasive and immuno-resistant phenotypes of Neisseria gonorrhoeae. EMBO (Eur. Mol. Biol. Organ.) J. 12:4043-4051.
35. Aydintug, M.K., T.J. Inzana, T. Letonja, W.C. Davis, and L.B. Corbeil. 1989. Cross-reactivity of monoclonal antibodies to Escherichia coli J5 with heterologous gram-negative bacteria and extracted lipopolysaccharides. J. Infect. Dis. 160: 846-857.
36. Hitchcock, P.J., and T.M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
37. West, S.E.H., and V.L. Clark. 1989. Genetic loci and linkage associations in Neisseria gonorrhoeae and Neisseria meningitidis. Clin. Microbiol. Rev. 2:S92-S103.
38. Frasch, C.E., W.D. Zollinger, and J.T. Poolman. 1985. Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev. Infect. Dis. 7:504-510.