Reduced volume PCR amplification reactions using the AmpFℓSTR® Profiler Plus™ kit

Journal of Forensic Sciences

Volumn 47, Issue 6, 2002, Pages 1224-1237

  Gaines, Michelle L ^a   Wojtkiewicz, Patrick W ^a   Valentine, Jennifer A ^a   Brown, Connie L ^a  

^a North Louisiana Criminalistics Laboratory   (United States)

Author keywords

DNA typing; Forensic science; Polymerase chain reaction; Reduced volume reaction; Sensitivity

Indexed keywords

ANALYTIC METHOD; ARTICLE; DEVICE; DNA DETERMINATION; DNA FINGERPRINTING; GENE AMPLIFICATION; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY;

ALLELES; DNA FINGERPRINTING; ELECTROPHORESIS; FORENSIC MEDICINE; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STATISTICS;

EID: 0036846228     PISSN: 00221198     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods In Enzymol 1987;155:335-50.
2. Jeffreys AJ, Wilson V, Neumann R, Keyte J. Amplification of human minisatellites by the polymerase chain reaction: Towards DNA finger-printing of single cells. Nucleic Acids Res 1988;16(23):10953-71.
3. Lins AM, Sprecher CJ, Puers C, Schumm JW. Multiplex sets for the amplification of polymorphic short tandem repeat loci - Silver stain and fluorescence detection. BioTechniques 1996;20(5):882-9.
4. Urquhart A, Oldroyd NJ, Kimpton CP, Gill P. Highly discriminating heptaplex short tandem repeat PCR system for forensic identification. BioTechniques 1995;18(1):116-21.
5. Reynolds R, Sensabaugh G, Blake E. Analysis of genetic markers in forensic DNA samples using the polymerase chain reaction. Anal Chem 1991;63:2-15.
6. Gill P, Sparkes R. Pinchin R, Clayton T, Whitaker J, Buckleton J. Interpreting simple STR mixtures using allele peak areas. Forensic Sci Int 1998;91:41-53.
7. Clayton TM, Whitaker JP, Sparkes R, Gill P. Analysis and interpretation of mixed forensic stains using DNA STR profiling. Forensic Sci Int 1998;91:55-70.
8. Sprecher CJ, Puers C, Lins AM, Schumm JW. General approach to analysis of polymorphic short tandem repeat loci. BioTechniques 1996;20 (2):266-76.
9. Edwards A, Civitello A, Hammond HA, Caskey CT. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Amer J Hum Genet 1991;49:746-56.
10. Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R. Evaluation of 13 short tandem repeat loci for use in personal identification applications. Amer J Hum Genet 1994;55:175-89.
11. Gill P, Whitaker J, Flaxman C. Brown N. Buckleton J. An investigation of the rigor of interpretation rules for STR's derived from less than 100 pg of DNA. Forensic Sci Int 2000;112:17-40.
12. Whitaker, JP, Cotton, EA, Gill P. A comparison of the characteristics of profiles produced with the AmpFℓSTRr® SGM Plus™ Multiplex System for both standard and Low Copy Number (LCN) STR DNA analysis. Forensic Sci Int 2001;123:215-23.
13. Ferreira-Gonzalez A, Yanovich S, Langley MR, Weymouth LA, Wilkinson DS, Garrett CT. Enhanced analytical sensitivity of a quantitative PCR for CMV using a modified nucleic-acid extraction procedure. J Clin Lab Anal 2000;14:32-7.
14. Leclair B, Foumey R. STR DNA typing: Increased sensitivity and efficient sample consumption using reduced PCR reaction volumes for database analysis, and casework implications. Proceedings of the 9th International Symposium on Human Identification; 7-10 Oct. 1998: Orlando. Madison (WI): Promega Corporation, 1999, (abstract).
15. Borys S. Bowen KL, Foumey RM. PCR volume reduction study using bloodstained FFA™ collection cards and capillary electrophoresis. Proceedings of the 9th International Symposium on Human Identification; 7-10 Oct. 1998; Orlando. Madison (WI): Promega Corporation, 1999, (abstract).
16. Comey CT, Budowle B. Validation studies on the analysis of the HLA DQα locus using the polymerase chain reaction. J Forensic Sci 1991;36 (6):1633-48.
17. Sambrook J, Maniatis T, Fritsch EF. Molecular Cloning (A Laboratory Manual). New York: Cold Spring Harbor Laboratory, 1982.
18. Hopwood A, Oldroyd N, Fellows S, Ward R, Owen SA, Sullivan K. Rapid quantification of DNA samples extracted from buccal scrapes prior to DNA profiling. BioTechniques 1997;23(1):18-20.
19. PE Applied Biosystems. AmpFℓSTRr® Profiler Plus™ PCR Amplification Kit User's Manual. The Perkin Elmer Corporation, 1997.
20. Wojtkiewicz, PW. Pituitary Gene Expression in Mice with Altered Growth Hormone Levels: Quantitative RT-PCR of Transcription Factors Pit-1, Zn-16, TEF, and Prop-1 [Dissertation]. New Orleans (LA): Tulane University, 1999.
21. Gaines ML, Wojtkiewicz P. Internal validation of the AmpFℓSTR Profiler Plus™ and COfiler™ PCR amplification kits. Proceedings of the American Academy of Forensic Sciences; 15-20 Feb. 1999; Orlando. Colorado Springs: American Academy of Forensic Sciences, 1999, (abstract).
22. Walsh PS, Erlich HA, Higuchi R. Preferential PCR amplification of alleles: Mechanisms and solutions. PCR Meth and Appl 1992;1:241-50.
23. Gill P, Sparkes R, Fereday L, Werrett DJ. Report of the European Network of Forensic Science Institute (ENSFI): Formulation and testing of principles to evaluate STR multiplexes. Forensic Sci Int 2000;108:1-29.