Rapid quantification of DNA samples extracted from buccal scrapes prior to DNA profiling

BioTechniques

Volumn 23, Issue 1, 1997, Pages 18-20

  Hopwood, A ^a   Oldroyd, N ^a   Fellows, S ^a   Ward, R ^a   Owen, S A ^a   Sullivan, K ^a  

^a FORENSIC SCIENCE SERVICE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA DETERMINATION; GENE AMPLIFICATION; GENE LOCUS; HUMAN; MOUTH CAVITY; QUANTITATIVE ASSAY;

EID: 0030792979     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97231bm01     Document Type: Article

References (6)

1. Gill, P., C.P. Kimpton, A.J. Urquhart, N.J. Oldroyd, E.S. Millican, S.K. Watson and T.J. Downes. 1995. Automated short tandem repeat (STR) analysis in forensic casework - a strategy for the future. Electrophoresis 16: 1543-1552.
2. Haughland, R. 1996. Handbook of Fluorescent Probes and Research Chemicals, 6th ed. Molecular Probes, Eugene, OR.
3. Kimpton, C.P., N.J. Oldroyd, S.K. Watson, R.R.E. Frazier, P.E. Johnson, E.S. Millican, A.J. Urquhart, B.L. Sparkes and P. Gill. 1996. Validation of highly discriminating multiplex short tandem repeat amplification systems for individual identification. Electrophoresis 17:1283-1293.
4. Walsh, P.S., D.A. Metzger and R. Higuchi. 1991. Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506-513.
5. Walsh, P.S., J. Varlaro and R. Reynolds. 1992. A rapid chemiluminescent method for quantification of human DNA. Nucleic Acids Res. 20:5061-5065.
6. Waye, J.S., L.A. Presley, B. Budowle, G.G. Shutler and R.M. Fourney. 1989. A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts. BioTechniques 7:852-855.