Quantification of hepatitis C virus in human liver and serum samples by using LightCycler reverse transcriptase PCR

Journal of Clinical Microbiology

Volumn 40, Issue 11, 2002, Pages 4346-4348

  White, Peter A ^a   Pan, Yong ^a   Freeman, Anthony J ^a   Marinos, George ^a   Ffrench, Rosemary A ^a   Lloyd, Andrew R ^a   Rawlinson, William D ^a  

^a PRINCE OF WALES HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ARTICLE; CORRELATION ANALYSIS; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; HUMAN TISSUE; INFECTION RISK; LIVER; NONHUMAN; PATIENT MONITORING; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; VIREMIA; VIRUS IDENTIFICATION; VIRUS LOAD;

HEPACIVIRUS; HEPATITIS C, CHRONIC; HUMANS; LIVER; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD; VIREMIA;

HEPATITIS C VIRUS; RNA VIRUSES;

EID: 0036840157     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.11.4346-4348.2002     Document Type: Article

References (22)

1. Beld, M., R. Sentjens, S. Rebers, C. Weegink, J. Weel, C. Sol, and R. Boom. 2002. Performance of the new Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: Conversion to international units and comparison with the Roche COBAS Amplicor HCV monitor, version 2.0, assay. J. Clin. Microbiol. 40:788-793.
2. Choo, Q. L., G. Kuo, A. J. Weiner, L. R. Overby, D. W. Bradley, and M. Houghton. 1989. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359-362.
3. De Moliner, L., P. Pontisso, G. L. De Salvo, L. Cavalletto, L. Chemello, and A. Alberti. 1998. Serum and liver HCV RNA levels in patients with chronic hepatitis C: Correlation with clinical and histological features. Gut 42:856-860.
4. Detmer, J., R. Lagier, J. Flynn, C. Zayati, J. Kolberg, M. Collins, M. Urdea, and R. Sanchez-Pescador. 1996. Accurate quantification of hepatitis C virus (HCV) RNA from all HCV genotypes by using branched-DNA technology. J. Clin. Microbiol. 34:901-907.
5. Freeman, A. J., G. J. Dore, M. G. Law, M. Thorpe, J. Von Overbeck, A. R. Lloyd, G. Marinos, and J. M. Kaldor. 2001. Estimating progression to cirrhosis in chronic hepatitis C virus infection. Hepatology 34:809-816.
6. Freeman, A. J., A. Zekry, L. R. Whybin, C. E. Harvey, I. A. van Beek, S. L. de Kantzow, W. D. Rawlinson, C. R. Boughton, P. W. Robertson, G. Marinos, and A. R. Lloyd. 2000. Hepatitis C prevalence among Australian injecting drug users in the 1970s and profiles of virus genotypes in the 1970s and 1990s. Med. J. Aust. 172:588-591.
7. Hu, K. Q., C. H. Yu, and J. M. Vierling. 1993. One-step RNA polymerase chain reaction for detection of hepatitis C virus RNA. Hepatology 18:270-274.
8. Idrovo, V., P. J. Dailey, L. J. Jeffers, E. Coelholittle, D. Bernstein, M. Bartholomew, L. Alvarez, M. S. Urdea, M. L. Collins, and E. R. Schiff. 1996. Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. J. Viral Hepatitis 3:239-246.
9. Kishimoto, H., K. Yoshioka, M. Yano, K. Ukai, H. Ito, K. Watanabe, O. Kawamata, and S. Kakumu. 2001. Real-time detection system for quantitation of hepatitis C virus RNA: A comparison with the other three methods. Hepatol. Res. 19:12-21.
10. Komurian-Pradel, F., G. Paranhos-Baccala, M. Sodoyer, P. Chevallier, B. Mandrand, V. Lotteau, and P. Andre. 2001. Quantitation of HCV RNA using real-time PCR and fluorimetry. J. Virol. Methods 95:111-119.
11. Kuo, G., Q. L. Choo, H. J. Alter, G. L. Gitnick, A. G. Redeker, R. H. Purcell, T. Miyamura, J. L. Dienstag, M. J. Alter, C. E. Stevens, et al. 1989. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362-364.
12. Martell, M., J. Gomez, J. I. Esteban, S. Sauleda, J. Quer, B. Cabot, R. Esteban, and J. Guardia. 1999. High-throughput real-time reverse transcription PCR quantitation of hepatitis C virus RNA. J. Clin. Microbiol. 37:327-332.
13. McGuinness, P. H., G. A. Bishop, D. M. Painter, R. Chan, and G. W. McCaughan. 1996. Intrahepatic hepatitis C RNA levels do not correlate with degree of liver injury in patients with chronic hepatitis C. Hepatology 23:676-687.
14. Mellor, J., A. Hawkins, and P. Simmonds. 1999. Genotype dependence of hepatitis C virus load measurement in commercially available quantitative assays. J. Clin. Microbiol. 37:2525-2532.
15. Nolte, F. S., C. Thurmond, and M. W. Fried. 1995. Preclinical evaluation of Amplicor hepatitis C virus test for detection of hepatitis C virus RNA. J. Clin. Microbiol. 33:1775-1778.
16. Takeuchi, T., A. Katsume, T. Tanaka, A. Abe, K. Inoue, K. TsukiyamaKohara, R. Kawaguchi, S. Tanaka, and M. Kohara. 1999. Real-time detection system for quantification of hepatitis C virus genome. Gastroenterology 116:636-642.
17. Tanaka, E., C. Ohue, K. Aoyagi, K. Yamaguchi, S. Yagi, K. Kiyosawa, and H. J. Alter. 2000. Evaluation of a new enzyme irnmunoassay for hepatitis C virus (HCV) core antigen with clinical sensitivity approximating that of genomic amplification of HCV RNA. Hepatology 32:388-393.
18. Terrault, N. A., P. J. Dailey, L. Ferrell, M. L. Collins, J. C. Wilber, M. S. Urdea, B. N. Bhandari, and T. L. Wright. 1997. Hepatitis C virus - Quantitation and distribution in liver. J. Med. Virol. 51:217-224.
19. White, P. A., X. Zhai, I. Carter, Y. Zhao, and W. D. Rawlinson. 2000. Simplified hepatitis C virus genotyping using heteroduplex mobility analysis. J. Clin. Microbiol. 38:477-482.
20. Yamada, G., M. Takatani, F. Kishi, M. Takahashi, T. Doi, T. Tsuji, S. Shin, M. Tanno, M. S. Urdea, and J. A. Kolberg. 1995. Efficacy of interferon alfa therapy in chronic hepatitis C patients depends primarily on hepatitis C virus RNA level. Hepatology 22:1351-1354.
21. Young, K. K., R. M. Resnick, and T. W. Myers. 1993. Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay. J. Clin. Microbiol. 31:882-886.
22. Zeuzem, S., J. H. Lee, A. Franke, B. Ruster, O. Prummer, G. Herrmann, and W. K. Roth. 1998. Quantification of the initial decline of serum hepatitis C virus RNA and response to interferon alfa. Hepatology. 27:1149-1156.