Single live cell imaging for real-time monitoring of resistance mechanism in Pseudomonas aeruginosa

Journal of Biomedical Optics

Volumn 7, Issue 4, 2002, Pages 576-586

  Kyriacou, Sophia V ^a   Nowak, Michelle E ^a   Brownlow, William J ^a   Xu, Xiao Hong Nancy ^a  

^a OLD DOMINION UNIVERSITY   (United States)

Author keywords

Efflux pump; Fluorescence microscopy and spectroscopy; Live cell imaging; Multi antibiotics resistance; Pseudomonas aeruginosa; Single cell analysis

Indexed keywords

ANTIBIOTICS; BACTERIA; CELL CULTURE; FLUORESCENCE; MICROSCOPIC EXAMINATION; REACTION KINETICS; SPECTROSCOPIC ANALYSIS; SUBSTRATES;

SINGLE CELL ANALYSIS;

MOLECULAR BIOLOGY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

ETHIDIUM; FLUORESCENT DYE;

ANTIBIOTIC RESISTANCE; ARTICLE; CYTOLOGY; DRUG EFFECT; FLUORESCENCE MICROSCOPY; METABOLISM; METHODOLOGY; OPTICS; PSEUDOMONAS AERUGINOSA; SPECTROFLUOROMETRY; STATISTICAL ANALYSIS; STATISTICS;

DATA INTERPRETATION, STATISTICAL; DRUG RESISTANCE, BACTERIAL; ETHIDIUM; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; OPTICS; PSEUDOMONAS AERUGINOSA; SPECTROMETRY, FLUORESCENCE;

EID: 0036816608     PISSN: 10833668     EISSN: None     Source Type: Journal    
DOI: 10.1117/1.1506707     Document Type: Article

References (25)

1. T. Nakae, "Multiantibiotic resistance caused by active drug extrusion in Pseudomonas aeruginosa and other gram-negative bacteria," Microbiologia 13(3), 273-284 (1997), and references therein.
2. S.P. Cole, G. Bhardwaj, J.H. Gerlach, J.E. Mackie, C.E. Grant, K.C. Almquist, A.J. Stewart, E.U. Kurz, A.M. Duncan, and R.G. Deeley, "Over expression of a transporter gene in a mutidrug-resistant human lung cancer cell line," Science 258, 1650-1654 (1992).
3. For review: B.M. Byan, T.J. Dougherty, D. Beaulieu, J. Chuang, B.A. Dougherty, and J.F. Barrett, "Efflux in bacteria: what do we really know about it?" Expert Opin. 10(7), 1409-1422 (2001), and references therein.
4. D. Ma, D.N. Cook, J.E. Hearst, and H. Nikaido, "Efflux pumps and drug resistance in gram-negative bacteria," Trends Microbiol. 2(12), 489-493 (1994).
5. H. Maseda, H. Yoneyama, and T. Nakae, "Assignment of the snbstrate-selective subunits of the MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa," Antimicrob. Agents Chemother. 44(3), 658-664 (2000).
6. S.R. Morshed, Y. Lei, H. Yoneyama, and T. Nakae, "Expression of genes associated with antibiotic extrusion in Pseudomonas aeruginosa," Biochem. Biophys. Res. Commun. 210(2), 356-362 (1995).
7. Y. Lei, K. Sato, and T. Nakae, "Ofloxacin-resistant Pseudomonas aeruginosa mutants with elevated drug extrusion across the inner membrane," Biochem. Biophys. Res. Commun. 178(3), 1043-1048 (1991).
8. T. Nakae, E. Yoshihara, and H. Yoneyama, "Multiantibiotic resistance caused by active drug extrusion in hospital pathogens," J. Infect. Chemother 3, 173-183 (1997), and references therein.
9. For review: T. Nakae, "Role of membrane permeability in determining antibiotic resistance in Pseudomonas aeruginosa," Microbiol. Immunol. 39(4), 221-229 (1995), and references therein.
10. A. Lee, W. Mao, M.S. Warren, A. Mistry, K. Hoshino, R. Okumura, H. Ishida, and O. Lomovskaya, "Interplay between efflux pumps may provide either additive or multiplicative effects on drug resistance," J. Bacteriol. 182(11), 3142-3150 (2000).
11. M. Germ, E. Yoshihara, H. Yoneyama, and T. Nakae, "Interplay between the efflux pump and the outer membrane permeability barrier in fluorescent dye accumulation in Pseudomonas aeruginosa," Biochem. Biophys. Res. Commun. 261(2), 452-455 (1999).
12. T. Kitahara, H. Yoneyama, and T. Nakae, "Antibiotic diffusion pathways in the outer membrane of Pseudomonas aeruginosa," Biochem. Biophys. Res. Commun. 238(2), 457-461 (1997).
13. N. Masuda, E. Sakagawa, S. Ohya, N. Gotoh, H. Tsujimoto, and T. Nishino, "Substrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-oprM efflux pumps in Pseudomonas aeruginosa," Agents Chemother. 44(12), 3322-3327 (2000).
14. P.G. Mortimer and L.J. Piddock, "A comparison of methods used for measuring the accumulation of quinolones by Enterobacteriaceae, Pseudomonas aeruginosa and Staphylococcus aureus," J. Antimicrob. Chemother. 28(5), 639-653 (1991).
15. A. Ocaktan, H. Yoneyama, and T. Nakae, "Use of fluorescence probes to monitor function of the subunit proteins of the MexA-MexB-oprM drug extrusion machinery in Pseudomonas aeruginosa," J. Biol. Chem. 272(35), 21964-21969 (1997).
16. H. Yoneyama, H. Maseda, H. Kamiguchi, and T. Nakae, "Function of the membrane fusion protein, MexA, of the MexA, B-OprM efflux pump in Pseudomonas aeruginosa without an Anchoring Membrane," J. Biol. Chem. 275(7), 4628-4634 (2000).
17. H. Morjani, N. Aouali, R. Belhoussine, R.J. Veldman, T. Levade, and M. Manfait, "Elevation of glucosylceramide in multidrug-resistant cancer cells and accumulation in cytoplasmic droplets," Int. J. Cancer 94(2), 157-65 (2001), and references therein.
18. E. Crivellato, L. Candussio, A.M. Rosati, F. Bartoli-Klugmann, F. Mallardi, and G. Decorti, "The fluorescent probe Bodipy-FL-verapamil is a substrate for both P-glycoprotein and multidrug resistance-related protein (MRP)-1," J. Histochem. Cytochem. 50(5), 731-734 (2002), and references therein.
19. C.K. Stover, X.Q. Pham, A.L. Erwin, S.D. Mizoguchi, P. Warrener, M.J. Hickey, F.S. Brinkman, W.O. Hufnagle, D.J. Kowalik, M. Lagrou, R.L. Gather, L. Goltry, E. Tolentino, S. Westbrock-Wadman, Y. Yuan, L.L. Brody, S.N. Coulter, K.R. Folger, A. Kas, K. Larbig, R. Lim, K. Smith, D. Spencer, G.K. Wong, Z. Wu, and I.T. Paulsen, Nature (London) 406, 959-964 (2000); I.T. Paulsen, M.K. Sliwinski, and M.H. Saier, Jr., J. Mol. Biol. 277, 573-592 (1998).
20. C.K. Stover, X.Q. Pham, A.L. Erwin, S.D. Mizoguchi, P. Warrener, M.J. Hickey, F.S. Brinkman, W.O. Hufnagle, D.J. Kowalik, M. Lagrou, R.L. Gather, L. Goltry, E. Tolentino, S. Westbrock-Wadman, Y. Yuan, L.L. Brody, S.N. Coulter, K.R. Folger, A. Kas, K. Larbig, R. Lim, K. Smith, D. Spencer, G.K. Wong, Z. Wu, and I.T. Paulsen, Nature (London) 406, 959-964 (2000); I.T. Paulsen, M.K. Sliwinski, and M.H. Saier, Jr., J. Mol. Biol. 277, 573-592 (1998).
21. A.R. Morgan, J.S. Lee, D.E. Pulleyblank, N.L. Murray, and D.H. Evans, "Review: ethidium fluorescence assays. Part 1. Physicochemical studies," Nucleic Acids Res. 7(3), 547-569 (1979).
22. W.J. Brownlow, M.E. Nowak, Q. Wan, and X.-H.N. Xu (unpublished).
23. K. Saito, H. Yoneyama, and T. Nakae, "nalB-type mutations causing the overexpression of the MexAB-OprM efflux pump are located in the mexR gene of the Pseudomonas aeruginosa chromosome," FEMS Microbiol. Lett. 179(1), 67-72 (1999).
24. D. Freifelder, Physical Biochemistry, pp. 51 and 52, Freeman, New York (1982).
25. X.-H.N. Xu, J. Chen, R. Jeffers, and S. Kyriacou, "Direct measurement of sizes and dynamics of single living membrane transporters using nano-optics," Nano Lett. 2, 175-182 (2002).