The cyanobacterial PilT protein responsible for cell motility and transformation hydrolyzes ATP

Plant and Cell Physiology

Volumn 43, Issue 10, 2002, Pages 1127-1136

  Okamoto, Shinobu ^a   Ohmori, Masayuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

ATPase; Cyanobacteria; Motility; Pili; pilT

Indexed keywords

CYANOBACTERIA; ESCHERICHIA COLI; MYXOCOCCUS XANTHUS; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; SYNECHOCYSTIS; SYNECHOCYSTIS SP.; SYNECHOCYSTIS SP. PCC 6803;

EID: 0036808835     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/pcp/pcf128     Document Type: Article

References (35)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
2. Bhaya, D., Bianco, N.R., Bryant, D. and Grossman, A. (2000) Type IV pilus biogenesis and motility in the cyanobacterium Synechocystis sp. PCC 6803. Mol. Microbiol. 37: 941-51.
3. Bhaya, D., Watanabe, N., Ogawa, T. and Grossman, A.R. (1999) The role of an alternative sigma factor in motility and pilus formation in the cyanobacterium Synechocystis sp. strain PCC 6803. Proc. Natl. Acad. Sci. USA 96: 3188-3193.
4. Bradley, D.E. (1972) Shortening of Pseudomonas aeruginosa pili after RNA-phage adsorption. J. Gen. Microbiol. 72: 303-319.
5. Brahamsha, B. (1996a) A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus. Appl. Environ. Microbiol. 62: 1747-1751.
6. Brahamsha, B. (1996b) An abundant cell-surface polypeptide is required for swimming by the nonflagellated marine cyanobacterium Synechococcus. Proc. Natl. Acad. Sci. USA 93: 6504-6509.
7. Chung, Y.S., Breidt, F. and Dubnau, D. (1998) Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis. Mol. Microbiol. 29: 905-913.
8. Chung, Y.S. and Dubnau, D. (1998) All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis. J. Bacteriol. 180: 41-45.
9. Elhai, J. and Wolk, C.P. (1988) A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138.
10. Halfen, L.N. and Castenholz, R.W. (1970) Gliding in a blue-green alga: A possible mechanism. Nature 225: 1163-1165.
11. Hihara, Y. and Ikeuchi, M. (1997) Mutation in a novel gene required for photomixotrophic growth leads to enhanced photoautotrophic growth of Synechocystis sp. PCC 6803. Photosynth. Res. 53: 243-252.
12. Hihara, Y., Sonoike, K. and Ikeuchi, M. (1998) A novel gene, pmgA, specifically regulates photosystem stoichiometry in the cyanobacterium Synechocystis species PCC 6803 in response to high light. Plant Physiol. 117: 1205-1216.
13. Hoiczyk, E. (1998) Structural and biochemical analysis of the sheath of Phormidium uncinatum. J. Bacteriol. 180: 3923-3932.
14. 2+-binding glycoprotein essential for the gliding motility of cyanobacteria. Mol. Microbiol. 26: 699-708.
15. Hoiczyk, E. and Baumeister, W. (1998) The junctional pore complex, a prokaryotic secretion organelle, is the molecular motor underlying gliding motility in cyanobacteria. Curr. Biol. 8: 1161-1168.
16. Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., Kimura, T., Hosouchi, T., Matsuno, A., Muraki, A., Nakazaki, N., Naruo, K., Okumura, S., Shimpo, S., Takeuchi, C., Wada, T., Watanabe, A., Yamada, M., Yasuda, M. and Tabata, S. (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3: 109-136.
17. Lanzetta, P.A., Alvarez, L.J., Reinach, P.S. and Candia, O.A. (1979) An improved assay for nanomole amounts of inorganic phosphate. Anal. Biochem. 100: 95-97.
18. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Merz, A.J., So, M. and Sheetz, M.P. (2000) Pilus retraction powers bacterial twitching motility. Nature. 407: 98-102.
20. Okamoto, S., Ikeuchi, M. and Ohmori, M. (1999) Experimental analysis of recently transposed insertion sequences in the Synechocystis sp. PCC 6803. DNA Res. 6: 265-273.
21. Okamoto, S. and Ohmori, M. (1999) Analysis of cyanobacterial motility in Synechocystis sp. PCC 6803. Plant Cell Physiol. 40 Suppl.: 135S.
22. Possot, O. and Pugsley, A.P. (1994) Molecular characterization of PulE, a protein required for pullulanase secretion. Mol. Microbiol. 12: 287-299.
23. Pugsley, A.P. (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57: 50-108.
24. Russel, M. (1998) Macromolecular assembly and secretion across the bacterial cell envelope: Type II protein secretion systems. J. Mol. Biol. 279: 485-499.
25. Skerker, J.M. and Berg, H.C. (2001) Direct observation of extension and retraction of type IV pili. Proc. Natl. Acad. Sci. USA. 98: 6901-6904.
26. Tang, Y.W-J.Y (1993) Blot-affinity purification of antibodies. In Methods in Cell Biology vol. 37. pp. 95-104, Academic Press, Inc. New York.
27. Terauchi, K. and Ohmori, M. (1999) An adenylate cyclase, Cyal, regulates cell motility in the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 40: 248-251.
28. Vaara, T. and Vaara, M. (1988) Cyanobacterial fimbriae. Methods Enzymol. 167: 189-195.
29. Walker, J.E., Saraste, M., Runswick, M.J. and Gay, N.J. (1982) Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1: 945-951.
30. Wall, D. and Kaiser, D. (1999) Type IV pili and cell motility. Mol. Microbiol. 32: 1-10.
31. Whitchurch, C.B., Hobbs, M., Livingston, S.P., Krishnapillai, V. and Mattick, J.S. (1991) Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria. Gene 101: 33-44.
32. Williams, J.G.K. (1988) Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering. Methods Enzymol. 167: 766-778.
33. Wolfgang, M., Lauer, P., Park, H.S., Brossay, L., Hebert, J. and Koomey, M. (1998) PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae. Mol. Microbiol. 29: 321-330.
34. Wu, S.S. and Kaiser, D. (1995) Genetic and functional evidence that Type IV pili are required for social gliding motility in Myxococcus xanthus. Mol. Microbiol. 18: 547-58.
35. Wu, S.S., Wu, J. and Kaiser, D. (1997) The Myxococcus xanthus pilT locus is required for social gliding motility although pili are still produced. Mol. Microbiol. 23: 109-121.