Determination of the stoichiometry of noncovalent complexes using reverse-phase high-performance liquid chromatography coupled with electrospray ion trap mass spectrometry

Analytical Biochemistry

Volumn 309, Issue 1, 2002, Pages 11-18

  Orsatti, Laura ^a   Di Marco, Stefania ^a   Volpari, Cinzia ^a   Vannini, Alessandro ^a   Neddermann, Petra ^a   Bonelli, Fabio ^a  

^a IRBM   (Italy)

Author keywords

Liquid chromatography; Mass spectrometry; Noncovalent complex; NS3; Stoichiometry; TraR

Indexed keywords

CALIBRATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; LIQUIDS; MASS SPECTROMETERS; MASS SPECTROMETRY; PROTEINS; STOICHIOMETRY; VIRUSES;

ACYL HOMOSERINE LACTONES; ELECTROSPRAY MASS SPECTROMETRY; ION TRAP MASS SPECTROMETER; NON-COVALENT COMPLEXES; REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SELECTED REACTION MONITORING; TRANSCRIPTIONAL REGULATOR; TRAR;

MOLAR CONCENTRATION;

HOMOSERINE; LACTONE DERIVATIVE; PROTEINASE; CARRIER PROTEIN; DRUG DERIVATIVE; ESCHERICHIA COLI PROTEIN; NS3 PROTEIN, HEPATITIS C VIRUS; NS4A COFACTOR PEPTIDE, HEPATITIS C VIRUS; OUTER MEMBRANE PROTEIN; TRAT PROTEIN, E COLI; VIRUS PROTEIN;

ANALYTIC METHOD; ARTICLE; CALCULATION; CALIBRATION; COMPLEX FORMATION; COVALENT BOND; DISSOCIATION; HEPATITIS C VIRUS; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN FAMILY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY; SPECTROMETER; STOICHIOMETRY; AMINO ACID SEQUENCE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; RHIZOBIUM RADIOBACTER;

AGROBACTERIUM; HEPATITIS C VIRUS;

AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; CALIBRATION; CARRIER PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI PROTEINS; HEPACIVIRUS; HOMOSERINE; HYDROPHOBICITY; MOLECULAR SEQUENCE DATA; RHIZOBIUM RADIOBACTER; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; VIRAL NONSTRUCTURAL PROTEINS; VIRAL PROTEINS;

EID: 0036808576     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00295-6     Document Type: Article

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