Cloning and characterization of a cDNA encoding an antimicrobial protein from mung bean seeds

Botanical Bulletin of Academia Sinica

Volumn 43, Issue 4, 2002, Pages 251-259

  Chen, Kuan Chung ^a   Lin, Ching Yu ^a   Chung, Mei Chu ^a   Kuan, Cheng Chun ^a   Sung, Hsien Yi ^a   Tsou, Samson C S ^a   Kuo, C George ^a   Chen, Ching San ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Antimicrobial protein; Immunolocalization; In situ hybridization; Mung bean

Indexed keywords

COMPLEMENTARY DNA; PROTEIN VRCRP; VEGETABLE PROTEIN;

ANTIMICROBIAL ACTIVITY; CELL GROWTH; GENBANK; GROWTH INHIBITION; INSECT CELL; ISOELECTRIC POINT; MOLECULAR WEIGHT; MUNG BEAN; NEAR ISOGENIC LINE; NORTHERN BLOTTING; PLANT PARENCHYMA CELL; PLANT SEED; PROTEIN LOCALIZATION;

ESCHERICHIA COLI; RHIZOCTONIA SOLANI; SPODOPTERA FRUGIPERDA;

EID: 0036807175     PISSN: 00068063     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Bloch, C. Jr. and M. Richardson. 1991. A new family of small (5 kDa) protein inhibitors of insect α-amylases from seeds of sorghum (Sorghum bicolor (L.) Moench) have sequence homologies with wheat γ-purothionins. FEBS Lett. 279: 101-104.
2. Bohlmann, H. 1994. The role of thionins in plant protection. Critical Rev. Plant Sci. 13: 1-16.
3. Borisjuk, L., H. Weber, R. Panitz, R. Manteuffel, and U. Wobus. 1995. Embryogenesis of Vicia faba L.: Histodifferentiation in relation to starch and storage protein synthesis. J. Plant Physiol. 147: 203-218.
4. Broekaert, W.F., F.R.G. Terras, B.P.A. Cammue, and R.W. Osborn. 1995. Plant defensins: Novel antimicrobial peptides as components of the host defense system. Plant Physiol. 108: 1353-1358.
5. Broekaert, W.F., B.P.A. Cammue, M.F.C. De Bolle, K. Thevissen, G.W. De Samblanx, and R.W. Osborn. 1997. Antimicrobial peptides from plants. Critical Rev. Plant Sci. 16: 297-323.
6. Cammue, B.P.A., M.F.C. De Bolle, F.R.G. Terras, P. Proost, J. Van Damme, S.B. Rees, J. Vanderleyden, and W.F. Broekaert. 1992. Isolation and characterization of a novel class of plant antimicrobial peptides from Mirabilis jalapa L. seeds. J. Biol. Chem. 267: 2228-2233.
7. Chomczynski, P. 1992. One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal. Biochem. 201: 134-139.
8. Chong, S., F.B. Mersha, D.G. Comb, M.E. Scott, D. Landry, L. M. Vence, F.B. Perler, J. Benner, R.B. Kucera, C.A. Hirvonen, J.J. Pelletier, H. Paulus, and M.Q. Xu. 1997. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192: 271-281.
9. Chung, R.P.T., G.M. Neumann, and G.M. Polya. 1997. Purification and characterization of basic proteins with in vitro antifungal activity from seeds of cotton, Gossypium hirsutum. Plant Sci. 127: 1-16.
10. Church, G.M. and W. Gilbert. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81: 1991-1995.
11. Colilla, F.J., A. Rocher, and E. Mendez. 1990. γ-Purothionins: Amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm. FEBS Lett. 270: 191-194
12. Duvick, J.P., T. Rood, A.G. Rao, and D.R. Marshak. 1992. Purification and characterization of a novel antimicrobial peptide from maize (Zea mays L.) kernels. J. Biol. Chem. 267: 18814-18820.
13. 2+ influx and phospholipase A2 activation and are inhibited by thionin tyrosine iodination. Proc. Natl. Acad. Sci. USA 86: 5849-5853.
14. Feinberg, A.P. and B. Vogelstein. 1984. A technique for radio-labeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal. Biochem. 137: 266-267.
15. Froy, O. and M. Gurevitz. 1998. Membrane potential modulators: A thread of scarlet from plants to humans. FASEB J. 12: 1793-1796.
16. Garcia-Olmedo, F., A. Molina, A. Segura, and M. Moreno. 1995. The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol. 3: 72-74.
17. Harrison, S.J., J.P. Marcus, K.C. Goulter, J.L. Green, D.J. Maclean, and J.M. Manners. 1997. An antimicrobial peptide from the Australian native Hardenbergia violacea provides the first functionally characterized member of a subfamily of plant defensins. Aust. J. Plant Physiol. 24: 571-578.
18. Hughes, P., E. Dennis, M. Whitecross, D. Llewellyn, and P. Gage. 2000. The cytotoxic plant protein, beta-purothionin, forms ion channels in lipid membranes. J. Biol. Chem. 275: 823-827.
19. Ishibashi, N., D. Yamauchi, and T. Minamikawa. 1990. Stored mRNA in cotyledons of Vigna unguiculata seeds: Nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination. Plant Mol. Biol. 15: 59-64.
20. Kobayashi, Y., H. Takashima, H. Tamaoki, Y. Kyogoku, P. Lambert, H. Kuroda, N. Chino, T.X. Watanabe, T. Kimura, S. Sakakibara, and L. Moroder. 1991. The cystine-stabilized α-helix: A common structural motif of ion-channel blocking neurotoxic peptides. Biopolymer 31: 1213-1220.
21. Kushmerick, C., M. de Souza Castro, J. Santos Cruz, C. Jr. Bloch, and P.S. Beirao. 1998. Functional and structural features of γ-zeathionins, a new class of sodium channel blockers. FEBS Lett. 440: 302-306.
22. Linthorst, H.J.M. 1991. Pathogenesis-related proteins of plants. Crit. Rev. Plant Sci. 10: 123-150.
23. Marcus, J.P., J.L. Green, K.C. Goulter, and J.M. Manners. 1999. A family of antimicrobial peptides is produced by processing of a 7S globulin protein in Macadamia integrifolia kernels. Plant J. 19: 699-710.
24. Ponstein, A.S., S.A. Bres-Vloemans, M.B. Sela-Buurlage, P.J.M. van den Elzen, L.S. Melchers, and B.J.C. Cornelissen. 1994. A novel pathogen- and wound-inducible tobacco (Nicotiana tabacum) protein with antifungal activity. Plant Physiol. 104: 109-118.
25. Saiki, R.K., D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G. T. Horn, K.B. Mullis, and H.A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491.
26. Schumbaum, A., F. Mauch, U. Vogeli, and T. Boller. 1896. Plant chitinases are potent inhibitors of fungal growth. Nature 324: 365-367.
27. Terras, F.R.G., K. Eggermont, V. Kovaleva, N.V. Raikhel, R.W. Osborn, A. Kester, S.B. Rees, S. Torrekens, F. Van Leuven, J. Vanderleyden, B.P.A. Cammue, and W.F. Broekaert. 1995. Small cysteine-rich antifungal proteins from radish: The role in host defense. Plant Cell 7: 573-588.
28. Terras, F.R.G., H.M.E. Schoofs, M.F.C. de Bolle, F. van L, S. B. Rees, J. Vanderleyden, B.P.A. Cammue, and E.F. Broekaert. 1992. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 267: 15301-15309.
29. Van Etten, H.D., D.E. Mattews, and P.S. Mattews. 1989. Phytoalexin detoxification: Importance for pathogenicity and practical implications. Annu. Rev. Phytopath. 27: 143-164.
30. Van Parijs, J., W.F. Broekaert, I.J. Goldstein, and W.J. Peumans. 1991. Hevein: An antifungal protein from rubber-tree (Hevea brasiliensis) latex. Planta 183: 258-264.
31. Verwocrd, T.C., B.M.M. Dekker, and A. Hockema. 1989. A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res. 17: 2362.
32. Zeng, L., R. Tate, and L.D. Smith. 1999. Capillary transfer as an efficient method of transferring proteins from SDS-PAGE gels to membranes. BioTechniques 26: 426-430.
33. Zhang, Y. and K. Lewis. 1997. Fabatins: New antimicrobial plant peptides. FEMS Microbiol Lett. 149: 59-64.