Capillary transfer as an efficient method of transferring proteins from SDS-PAGE gels to membranes

BioTechniques

Volumn 26, Issue 3, 1999, Pages 426-430

  Zeng, Lizhi ^a   Tate, Ron ^a   Smith, Larry D ^a  

^a HL Snyder Mem Research Foundation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OVALBUMIN; POLYVINYLIDENE FLUORIDE;

ARTICLE; ARTIFICIAL MEMBRANE; CAPILLARY ELECTROPHORESIS; ELECTROPHORETIC MOBILITY; INTERMETHOD COMPARISON; MEMBRANE BINDING; MEMBRANE TRANSPORT; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRODUCTIVITY; PROTEIN DETERMINATION; PROTEIN ISOLATION; PROTEIN TRANSPORT; TECHNIQUE;

EID: 0033047246     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99263bm12     Document Type: Article

References (5)

1. Millipore. 1996. Immobilon-CD Transfer Membrane and Immobilon-CD Stain User Guide. Bedford, MA.
2. Patterson, S.D., D. Hess, T. Yungwirth and R. Aebersold. 1992. High-yield recovery of electroblotted proteins and cleavage fragments from a cationic polyvinylidene fluoride-based membrane. Anal. Biochem. 202:193-203.
3. PE Applied Biosystems. 1991. ProBlott™ Applications in SDS-PAGE, Electroblotting and Protein Sequencing, User Bulletin, Foster City, CA.
4. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., p. 9.34-9.40. CSH Laboratory Press, Cold Spring Harbor, NY.
5. Warner, R.C. 1954. The Proteins, Vol. II. Part A, p. 443, Neurath-Baily, New York.