Real-time determination of telomerase activity in cell extracts using an optical biosensor

Biological Chemistry

Volumn 383, Issue 10, 2002, Pages 1659-1666

  Schmidt, Pete M ^a   Matthes, Eckart ^a   Scheller, Frieder W ^a   Bienert, Michael ^a   Lehmann, Christine ^a   Ehrlich, Angelika ^a   Bier, Frank F ^a  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

Author keywords

Biosensor; Grating coupler; Phosphorothioate modified oligonucleotides; Telomerase; TRAP assay

Indexed keywords

CELL EXTRACT; DNA; DNA POLYMERASE; NUCLEOTIDE; OLIGONUCLEOTIDE; PEPTIDE NUCLEIC ACID; PHOSPHOROTHIOIC ACID; TELOMERASE; BUFFER;

ARTICLE; BINDING AFFINITY; BINDING SITE; BIOSENSOR; CELL LYSATE; CELL STRAIN HL 60; CHEMICAL MODIFICATION; CONTROLLED STUDY; DISSOCIATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SUBSTRATE; GENE AMPLIFICATION; HUMAN; HUMAN CELL; IMMOBILIZATION; NUCLEIC ACID ANALYSIS; NUCLEIC ACID PROBE; NUCLEOTIDE METABOLISM; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SIGNAL NOISE RATIO; TELOMERE; TELOMERIC REPEAT AMPLIFICATION PROTOCOL; TUMOR CELL; VELOCITY; CELL CULTURE; CHEMISTRY; ENZYMOLOGY; FIBROBLAST; GENETIC PROCEDURES; GENETICS; INSTRUMENTATION; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OPTICS; PROTEIN BINDING; SENSITIVITY AND SPECIFICITY; SURFACE PROPERTY;

BASE SEQUENCE; BIOSENSING TECHNIQUES; BUFFERS; CELL EXTRACTS; FIBROBLASTS; HL-60 CELLS; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; OPTICS; PEPTIDE NUCLEIC ACIDS; PROTEIN BINDING; SENSITIVITY AND SPECIFICITY; SURFACE PROPERTIES; TELOMERASE; TUMOR CELLS, CULTURED;

EID: 0036806579     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2002.186     Document Type: Article

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