Stable augmentation of activated sludge with foreign catabolic genes harboured by an indigenous dominant bacterium

Environmental Microbiology

Volumn 4, Issue 10, 2002, Pages 577-583

  Watanabe, Kazuya ^a   Teramoto, Maki ^a   Harayama, Shigeaki ^a  

^a MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIODEGRADATION, ENVIRONMENTAL; CATALYSIS; COMAMONAS; GENES, BACTERIAL; GENETIC ENGINEERING; MIXED FUNCTION OXYGENASES; PHENOL; REFUSE DISPOSAL; SEWAGE;

BACTERIA (MICROORGANISMS); COMAMONAS SP.; COMAMONAS TESTOSTERONI;

EID: 0036801608     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-2920.2002.00342.x     Document Type: Article

References (27)

1. Davi, M.L., and Gnudi, F. (1999) Phenolic compounds in surface water. Wat Res 33: 3213-3219.
2. De Lorenzo, V., and Timmis, K.N. (1994) Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Meth Enzymol 235: 386-405.
3. Dejonghe, W., Goris, J., El Fantroussi, S., Hofte, M., de Vos, P., Verstraete, W., and Top, E.M. (2000) Effect of dissemination of 2,4-dichlorophenoxyacetic acid (2,4-D) degradation plasmids on 2,4-D degradation and on bacterial community structure in two different soil horizons. Appl Environ Microbiol 66: 3297-3304.
4. Eckenfelder, W.W., and O'Conner, D.J. (1961) Biological Waste Treatment. Pergamon: Oxford.
5. Erb, R.W., Eichner, C.A., Wagner-Dobler, I., and Timmis, K.N. (1997) Bioprotection of microbial communities from toxic phenol mixtures by a genetically designed pseudomonad. Nat Biotechnol 15: 378-382.
6. Haldimann, A., Prahalad, M.K., Fisher, S.L., Kim, S.-K., Walsh, C.T., and Wanner, B.L. (1996) Altered recognition mutants of the response regulator PhoB: a new genetic strategy for studying protein-protein interactions. Proc Natl Acad Sci USA 93: 14361-14366.
7. Hino, S., Watanabe, K., and Takahashi, N. (1998) Phenol hydroxylase cloned from Ralstonia eutropha strain E2 exhibits novel kinetic properties. Microbiology 144: 1765-1772.
8. Lee, S.Y., Bollinger, J., Bezdicek, D., and Ogram, A. (1996) Estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative PCR method Appl Environ Microbiol 62: 3787-3793.
9. Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208-218.
10. Muyzer, G., de Waal, E.C., and Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59: 695-700.
11. Olsen, R.H., DeBusscher, G., and McCombie, J.M. (1982) Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol 150: 60-69.
12. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
13. Sayler, G.S., and Ripp, S. (2000) Field application of genetically engineered microorganisms for bioremediation processes. Curr Opin Biotechnol 11: 286-289.
14. Sayler, G.S., Fleming, J.T., and Nivens, D.E. (2001) Gene expression monitoring in soils by mRNA and gene lux fusions. Curr Opin Biotechnol 12: 455-460.
15. Soda, S., Ike, M., and Fujita, M. (1998) Effects of inoculation of a genetically engineered bacterium on performance and indigenous bacteria of a sequencing batch activated sludge process treating phenol. J Ferment Bioeng 86: 90-96.
16. Suarez, A., Gutter, A., Strats, M., Staendner, L.H., Timmis, K.N., and Guzman, C.A. (1997) Green fluorescent protein-based reporter systems for genetic analysis of bacteria induding monocopy applications. Gene 196: 69-74.
17. Teramoto, M., Futamata, H., Harayama, S., and Watanabe, K. (1998) Characterization of a high-affinity phenol hydroxylase from Comamonas testosteroni R5 by gene cloning and expression in Pseudomonas aeruginosa PAO1c. Mol Gen Genet 262: 552-558.
18. Teramoto, M., Harayama, S., and Watanabe, K. (2001) PhcS represses gratuitous expression of phenol-metabolizing enzymes in Comamonas testosteroni R5. J Bacteriol 183: 4227-4234.
19. Teramoto, M., Ohnishi, K, Harayama, S., and Watanabe, K. (2002) An AraC/XylS family member at a high level in a Hierarchy of Regulators for phenol-metabolizing enzymes in Comamonas testosteroni R5. J Bacteriol 14: 3941-3946.
20. Van Veen, J.A., van Overbeek, L.S., and van Elsas, J.D. (1997) Fate and activity of microorganisms introduced into soil. Microbiol Mol Biol Rev 61: 121-135.
21. Watanabe, K. (2001) Microorganisms relevant to bioremediation. Curr Opin Biotechnol 12: 237-241.
22. Watanabe, K. Hino, S., Onodera, K., Kajie, S., and Takahashi, N. (1996a) Diversity in kinetics of bacterial phenol-oxygenating activity. J Ferment Bioeng 81: 562-565.
23. Watanabe, K, Hino, S., and Takahashi, N. (1996b) Effects of exogenous phenol-degrading bacteria on performance and ecosystem of activated sludge. J Ferment Bioeng 82: 291-298.
24. Watanabe, K., Yamamoto, S., Hino, S., and Harayama, S. (1998a) Population dynamics of phenol-degrading bacteria in activated sludge determined by gyrB-targeted quantitative PCR. Appl Environ Microbiol 64: 1203-1209.
25. Watanabe, K., Teramoto, M., Futamata, H., and Harayama, S. (1998b) Molecular detection, isolation, and physiological characterization of functionally dominant phenol-degrading bacteria in activated sludge. Appl Environ Microbiol 64: 4396-4402.
26. Watanabe, K., Teramoto, M., and Harayama, S. (1999) An outbreak of nonflocculating catabolic populations caused the breakdown of a phenol-digesting activated-sludge process. Appl Environ Microbiol 65: 2813-2819.
27. Watanabe, K., Miyashita, M., and Harayama, S. (2000) Reclamation of an activated-sludge microbial consortium by selective biostimulation. Appl Microbiol Biotechnol 54: 719-723.