Erythropoietin triggers a signaling pathway in endothelial cells and increases the thrombogenicity of their extracellular matrices in vitro

Thrombosis and Haemostasis

Volumn 88, Issue 4, 2002, Pages 678-685

  Fusté, Berta ^a   Serradell, Mireia ^a   Escolar, Ginés ^a   Cases, Aleix ^a   Mazzara, Roberto ^a   Castillo, Ricardo ^a   Ordinas, Antonio ^a   Díaz Ricart, Maribel ^a  

^a HOSPITAL CLÍNIC   (Spain)

Author keywords

Endothelium; Erythropoietin; Thrombosis; Tissue factor; Tyrosine phosphorylation

Indexed keywords

ANTIBODY; CYTOKINE; ERYTHROPOIETIN RECEPTOR; JAK 2 PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN; PROTEIN TYROSINE KINASE; RECOMBINANT ERYTHROPOIETIN; STAT5 PROTEIN; THROMBOPLASTIN; UNCLASSIFIED DRUG; ERYTHROPOIETIN; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; VON WILLEBRAND FACTOR;

ARTICLE; BLOOD; CELL CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DOSE RESPONSE; DRUG EFFECT; DRUG EXPOSURE; ENDOTHELIUM CELL; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RECEPTOR BINDING; SIGNAL TRANSDUCTION; THROMBOCYTE; THROMBOCYTE ADHESION; THROMBOGENICITY; CHEMICALLY INDUCED DISORDER; CHEMISTRY; CYTOLOGY; METABOLISM; PERFUSION; PHOSPHORYLATION; PHYSIOLOGY; THROMBOSIS; ULTRASTRUCTURE; UMBILICAL VEIN; VASCULAR ENDOTHELIUM;

BLOOD PLATELETS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOTHELIUM, VASCULAR; ERYTHROPOIETIN; EXTRACELLULAR MATRIX; HUMANS; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MITOGEN-ACTIVATED PROTEIN KINASES; PERFUSION; PHOSPHORYLATION; THROMBOPLASTIN; THROMBOSIS; UMBILICAL VEINS; VON WILLEBRAND FACTOR;

EID: 0036798849     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0037-1613275     Document Type: Article

References (49)

1. Kranz SB. Erythropoietin. Blood 1991; 77: 419-34.
2. Papayannopoulou T, Brice M, Farrer D, Kaushansky K. Insights into the cellular mechanism of erythropoietin-thrombopoietin synergy. Exp Hematol 1996; 24: 660-9.
3. Hawrylowicz CM. Viewpoint: A potencial role for platelets derived cytokines in the inflammatory response. Platelets 1993; 4: 1-10.
4. Eschbach JW, Egriev JC, Downing MR, Browne JK, Adamson JW. Correction of the anemia of end-stage renal disease with recombinant human erythropoietin: results of a combined phase I and II clinical trial. N Engl J Med 1987; 316: 73-8.
5. Remuzzi G. Bleeding in renal failure. Lancet 1988; 1: 1205-8.
6. Moia M, Mannucci PM, Vizzotto L, Casati S, Cattaneo M, Ponticelli C. Improvement in the haemostatic defect of uraemia after treatment with recombinant human erythropoeitin. Lancet 1987; 2: 1227-9.
7. Fernandez F, Goudable C, Sie P, Ton-That H, Durand D, Suc JM, Boneu B. Low hematocrit and prolonged bleeding time in uraemic patients: Effect of red cell transfusions. Br J Haematol 1985; 59: 139-48.
8. Zwaginga JJ, Ijsseldijk MJ, de Groot PG, Kooistra M, Vos J, vas Es A, Koomans HA, Struyvenberg A, Sixma JJ. Treatment of uremic anemia with recombinant erythropoietin also reduces the defects in platelet adhesion and aggregation caused by uremic plasma. Thromb Haemost 1991; 66: 638-47.
9. Cases A, Escolar G, Reverter JC, Ordinas A, Lopez-Pedret J, Revert L, Castillo R. Recombinant human erythropoietin treatment improves platelet function in uremic patients. Kidney Int 1992; 42: 668-72.
10. Diaz-Ricart M, Estebanell E, Cases A, Lopez-Pedret J, Castillo R, Ordinas A, Escolar G. Erythropoietin improves signaling through tyrosine phosphorylation in platelets from uremic patients. Thromb Haemost 1999; 82: 1312-7.
11. Jilma Stohlawetz P, Dzirlo L, Hergovich N, Lackner E, Mensik Ch, Eichelr HG, Kabrna E, Geissler K, Jilma B. Effects of erythropoietin on platelet reactivity and thrompoiesis in humans. Blood 2000; 95: 2983-9.
12. Anagnostou A, Lee ES, Kessimian N, Levinson R, Steiner M. Erythropoietin has a mitogenic and positive chemotactic effect on endothelial cells. Proc Natl Acad Sci USA 1990; 87: 587-91.
13. Carlini RG, Reyes AA, Rothstein M. Recombinant human erythropoietin stimulates angiogenesis in vitro. Kidney Int 1995; 47: 740-5.
14. Haller H, Christel C, Dannenberg L, Thiele P, Lindschau C, Luft FC. Signal transduction of erythropoietin in endothelial cells. Kidney Int 1996; 50: 481-8.
15. Gordge MP, Petel A, Faint RW, Rylance PB, Neild GH. Blood hyperviscosity and its relationship to progressive renal failure in patients with diabetic nephropathy. Diabet Med 1990, 7: 880-6.
16. Raine AEG. Hypertension, blood viscosity, and cardiovascular morbidity in the renal failure: Implications of erythropoietin therapy. Lancet 1988; 1: 97.
17. Baskin S, Lasker D. Erythropoietin-associated hypertension. N Engl J Med 1990; 323: 999-1000.
18. Heidenreich S, Rahn KH, Zidek W. Direct vasopressor effect of recombinant human erythropoietin on renal resistance vessels. Kidney 1991; 39: 259-65.
19. Singbartl G. Adverse events of erythropoietin in long term and in acute/short-term treatment. Clin Invest 1994; 72: S36-S43.
20. Serradell M, Díaz-Ricart M, Cases A, Zurbano MJ, Aznar-Salatti J, López-Pedret J, Ordinas A, Escolar G. Uremic medium disturbs the hemostatic balance of cultured human endothelial cells. Thromb Haemost 2001; 86: 1099-105.
21. Malyszko JS, Malyszko J, Pawlak K, Pawlak D, Buczko W, Mysliwiec M. Importance of serotonergic mechanisms in the thrombotic complications in hemodialyzed patients treated with erythropoietin. Nephron 2000; 84: 305-11.
22. Jaffe EA, Nachman RL, Becker CG. Culture of human endothelial cells derived from umbilical veins. J Clin Invest 1973; 52: 2745-56.
23. Aznar-Salatti J, Diaz-Ricart M, Anton P, Bastida E, Escolar G, Ordinas A. Variations in the thrombogenic properties of the extracellular matrix produced by endothelial cells in consecutive passages. Sangre. (Barc) 1990; 35: 173-7.
24. Towbin H, Stachelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose: Procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4.
25. Cichowski K, Brugge JS, Brass LF. Thrombin receptor activation and integrin engagement stimulate tyrosine phosphorylation of the proto-oncogene product, p95(vav), in platelets. J Biol Chem 1996; 271: 7544-50.
26. Sakariassen KS, Aarts PA, de Groot PG, Houdijk WP, Sixma JJ. A perfusion chamber developed to investigate platelet interaction in flowing blood with human vessel wall cells, their extracellular matrix, and purified components. J Lab Clin Med 1983; 102: 522-35.
27. Diaz-Ricart M, Carretero M, Castillo R, Ordinas A, Escolar G. Digital image analysis of platelet-extracellular matrix interactions: studies in von willebrand disease patients and aspirin-treated donors. Haemostasis 1994; 24: 219-29.
28. De Mey J, Moeremans M, Geuens G, Nuydens R, De Brabander M. High resolution light and electron microscopic localization of tubulin with the IGS (immunogold staining) method. Cell Biol Int Rep 1981; 5: 889-99.
29. Scopsi L, Larsson LI, Bastholm L, Nielsen MH. Silver-Enhanced colloidal gold probes as markers for scanning electron microscopy. Histochem J 1986; 86: 35-41.
30. Ihle J. Stats. Signal transducers and activators of transcription. Cell 1996; 84: 331-4.
31. Witthuhn BA, Quelle FW, Silvennoinen O, Yi T, Tang B, Miura O, Ihle J. JAK-2 associates with the erythropoietin receptor and is tyrosine phosphorylated and activated following stimulation with erythropoietin. Cell 1993; 74: 227-36.
32. Sabin F. Studies of the origin of blood vessels and of red corpuscles as seen in the living blastoderms of chicks during the second day of incubation. Contrib Embriol Carnege Inst Washington 1920; 9: 214.
33. Anagnostou A, Steiner M, Chin K, Lee ES, Kessimian N, Noguchi CT. Erythropoietin receptor mRNA expression in human endothelial cells. Proc Natl Acad Sci USA 1994; 91: 3974-8.
34. Ihle J. The Stat family in cytokine signaling. Curr Opin Cell Biol 2001; 13: 211-7.
35. Valdembri D, Serini G, Vacca A, Ribatti D, Bussolino F. In vivo activation of JAK2/STAT-3 pathway during angiogenesis induced by GM-CSF. FASEB J 2002; 16: 225-7.
36. Klingmüller U. The role of tyrosine phosphorylation in proliferation and maduration of erythroid progenitor cells: Signal emanating from the erythropoietin receptor. EJB 1997; 249: 637-47.
37. Metcalf D. The molecular control of cell division, differentiation, commitment and maturation in haematopoietic cells. Nature 1989; 339: 27-30.
38. Ratajckak J, Majka M. Kijowski J, Baj M, Pan ZK, Marquez LA, Janowska-Wieczorek A, Ratajczak MZ. Biological significance of MAPK, AKT and JAK-STAT protein activation by various erythropoietic factors in normal human early erythroid cells. Br J Haematol 2001; 115: 195-204.
39. Ribatti D, Presta M, Vacca A, Ria R, Giulani R, Dell'Era P, Nico B, Roncalli L, Dammaco F. Human erythropoietin induces a pro-angiogenic phenotype in cultured endothelial cells and stimulates neovascularization in vivo. Blood 1999; 93: 2627-36.
40. Beckman DL, Lin LL, Quinones ME, Longmore GD. Activation of the erythropoietin receptor is not required for internalization of bound erythropoietin. Blood 1999; 94: 2667-2675.
41. Nagata Y, Takahashi N, Davis RJ, Todokoro K. Activation of p38 MAP Kinase and JNK but not ERK is required for erythropoietin-induced erythropoietin differentiation. Blood 1998; 92: 1859-69.
42. Rao LVM, Rapaport SI, Bajaj SP. Activation of human factor VII in the initiation of tissue factor-dependent coagulation. Blood 1986; 65: 685-91.
43. Weiss HJ, Lages B. Evidence for tissue factor-dependent activation of the classic extrinsic coagulation mechanism in blood obtained from bleeding time wounds. Blood 1988; 71: 629-35.
44. Nemerson Y. Tissue factor and haemostasis. Blood 1988; 71: 1-8.
45. de Groot PG, Reinders JH, Sixma JJ. Perturbation of human endothelial cells by thrombin or PMA changes the reactivity of their extracellular matrix towards platelets. J Cell Biol 1987; 104: 697-704.
46. Drake TA, Morrissey JH, Edgington TS. Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis. Am J Pathol 1989; 134: 1087-97.
47. Mulder AB, Karin SM, Heggepaping KSM, Magielse CPE, Blom NR, Smit JW, Vandermeer J, Halie MR, Bom VJJ. Tumor necrosis factor α-induced endothelial tissue factor is located on the cell surface rather than in the subendothelial matrix. Blood 1994; 84: 1559-66.
48. Galvez A, Gomez-Ortiz G, Diaz-Ricart M, Escolar G, Gonzalez-Sarmiento R, Zurbano MJ, Ordinas A, Castillo R. Desmopressin (DDAVP) enhances platelet adhesion to the extracellular matrix of cultured human endothelial cells through increased expression of tissue factor. Thromb Haemost 1997; 77: 975-80.
49. Orvim U, Roald HE, Stephens RW, Roos N, Sakariassen KS. Tissue factor-induced coagulation triggers platelet thrombus formation as efficiently as fibrillar collagen at arterial blood flow conditions. Arterioscler Thromb 1994; 14: 1976-83.