A simple and reliable method for screening retroviral producer clones without selectable markers

Human Gene Therapy

Volumn 8, Issue 10, 1997, Pages 1189-1194

  Onodera, Masafumi ^a   Yachie, Akihiro ^b   Nelson, David M ^a   Welchlin, Heidi ^a   Morgan, Richard A ^a   Blaese, R Michael ^a  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^b KANAZAWA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC G 418; NEOMYCIN; PHOSPHOTRANSFERASE; VIRUS VECTOR;

ARTICLE; ASSAY; CELL CLONE; COLONY FORMING UNIT; DOT HYBRIDIZATION; GENE TRANSFER; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOGENICITY; NONHUMAN; RETROVIRUS; SOUTHERN BLOTTING;

UNIDENTIFIED RETROVIRUS;

EID: 0030881446     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1997.8.10-1189     Document Type: Article

References (20)

1. BUNNELL, B.A., MUUL, L.M., DONAHUE, R.E., BLAESE, R.M., and MORGAN, R.A. (1995). High-efficiency retroviral-mediated gene transfer into human and non-human primate peripheral blood lymphocytes. Proc. Natl. Acad. Sci. USA 92, 7739-7743.
2. CONE, R.D., and MULLIGAN, R.C. (1984). High-efficiency gene transfer into mammalian cells: generation of helper-free recombinant retrovirus with broad mammalian host range. Proc. Natl. Acad. Sci. USA 81, 6349-6353.
3. CRYSTAL, R.G. (1995). Transfer of genes to humans: early lessons and obstacles to success. Science 270, 404-410.
4. EMERMAN, M., and TEMIN, H.M. (1984). Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism. Cell 39, 459-467.
5. GORDON, E.M., and ANDERSON, W.F. (1994). Gene therapy using retroviral vectors. Curr. Opin. Biotechnol. 5, 611-616.
6. HOCK, R.A., MILLER, A.D., and OSBORNE, W.R.A. (1989). Expression of human adenosine deaminase from various strong promoters after gene transfer into human hematopoietic cell lines. Blood 74, 876-881.
7. HULL, R. (1985). Characterization of virus. In Virology, a Practical Approach. B.W.J. Mahy, ed. (IRL Press, Oxford, Washington DC), pp. 14-17.
8. HUSZAR, D., ELLIS, J., and BERNSTEIN, A. (1989). A rapid and accurate method for determining the titer of a retrovirus lacking a selectable marker. Technique J. Meth. Cell Mol. Biol. 1, 28-32.
9. KOHN, D.B., MITSUYA, H., BALLOW, M., SELENGUE, J.E., BARANKIEWICZ, J., COHEN, A., GELFAND, E., ANDERSON, W.F., and BLAESE, R.M. (1989). Establishment and characterization of adenosine deaminase-deficient human T cell lines. J. Immunol. 142, 3971-3977.
10. KOHN, D., WEINBERG, K., NOLTA, J., HEISS, L., LENARSKY, C., CROOKS, G., HANLEY, M., ANNETT, G., BROOKS, J., EL-KHOREIY, A., LAWRENCE, K., WELLS, W., MOEN., R., BASTIAN, J., WILLIAMS-HERMAN, D., ELDER, M., WARA, D., BOWEN, T., HERSHFIELD, M., MULLEN, C., BLAESE, R., and PARKMAN, R. (1995). Engraftment of gene-modified umbilical cord blood cells in neonates with adenosine deaminase deficiency. Nature Med. 1, 1017-1023.
11. KRALL, W.J., SKELTON, D.C., YU, X.J., RIVIERE, I., LEHN, P., MULLIGAN, R.C., and KOHN, D.B. (1996). Increased levels of spliced RNA account for augmented expression from the MFG retroviral vector in hematopoietic cells. Gene Ther. 3, 37-48.
12. MARKOWITZ, D., GOFF, S., and BANK, A. (1988). A safe packaging line for gene transfer: separating viral genes on two different plasmids. J. Virol. 62, 1120-1124.
13. von MELCHER, H., and HOUSEMAN, D. (1988). The expression of neomycin phosphotransferase in human promyelocytic leukemia cells (HL-60) delays their differentiation. Oncogene 2, 137-140.
14. MILLER, A.D., and BUTTIMORE, C. (1986). Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol. Cell Biol. 6, 2895-2902.
15. MILLER, A.D., GARCIA, J.V., VON SUHR, N., LYNCH, C.M., WILSON, C., and EIDEN, M.V. (1991). Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J. Virol. 65, 2220-2224.
16. MULLIGAN, R.C. (1993). The basic science of gene therapy. Science 260, 926-932.
17. OHASHI, T., BOGGS, S., ROBBINS, P., BAHNSON, A., PATRENE, K., WEI, F.S., WEI, J.F., LI, J., LUCHT, L., FEI, Y., CLARK, S., KIMAK, M., HE, H., MOWERY-RUSHTON, P., and BARRANGER, J.A. (1992). Efficient transfer and sustained high expression of the human glucocerebrosidase gene in mice and their functional macrophages following transplantation of bone marrow transduced by a retroviral vector. Proc. Natl. Acad. Sci. USA 89, 11332-11336.
18. RIDDELL, S.R., ELLIOTT, M., LEWINSOHN, D.A., GILBERT, M.J., WILSON, L., MANLEY, S.A., LUPTON, S.D., OVERELL, R.W., REYNOLDS, T.C., COREY, L., and GREENBERG, P.D. (1996). T-cell mediated rejection of gene-modified HIV-specific cytotoxic T lymphocytes in HIV-infected patients. Nature Med. 2, 216-223.
19. RIVIERE, I., BROSE, K., and MULLIGAN, R.C. (1995). Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells. Proc. Natl. Acad. Sci. USA 92, 6733-6737.
20. XU, L., YEE, J.K., WOLFF, J.A., and FRIEDMANN, T. (1989). Factors affecting long-term stability of Moloney murine leukemia virus-based vectors. Virology 171, 331-341.