Establishment of a novel collagenase perfusion method to isolate rat pancreatic stellate cells and investigation of their gene expression of TGF-β1, type I collagen, and CTGF in primary culture or freshly isolated cells

Acta Medica Okayama

Volumn 56, Issue 4, 2002, Pages 211-218

  Shinji, Toshiyuki ^a   Ujike, Kozo ^a   Ochi, Koji ^a   Kusano, Nobuchika ^a   Kikui, Tetsuya ^a   Matsumura, Naoki ^a   Emori, Yasuyuki ^a   Seno, Toshinobu ^a   Koide, Norio ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

Collagenase perfusion; Connective tissue growth factor; Pancreatic stellate cell; Transforming growth factor beta; WBN Kob rat

Indexed keywords

COLLAGEN TYPE 1; COLLAGENASE; CONNECTIVE TISSUE GROWTH FACTOR; IMMEDIATE EARLY PROTEIN; SIGNAL PEPTIDE; TGFB1 PROTEIN, RAT; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1;

ANIMAL; ARTICLE; CELL CULTURE; CELL SEPARATION; CYTOLOGY; FIBROSIS; GENE EXPRESSION; GENETICS; IMMUNOLOGICAL PROCEDURES; MALE; METHODOLOGY; PANCREAS; PATHOLOGY; PERFUSION; PHASE CONTRAST MICROSCOPY; PHYSIOLOGY; RAT; RAT STRAIN; WISTAR RAT;

ANIMALS; CELL SEPARATION; CELLS, CULTURED; COLLAGEN TYPE I; COLLAGENASES; FIBROSIS; GENE EXPRESSION; IMMEDIATE-EARLY PROTEINS; IMMUNOLOGIC TECHNIQUES; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MALE; MICROSCOPY, PHASE-CONTRAST; PANCREAS; PERFUSION; RATS; RATS, INBRED STRAINS; RATS, WISTAR; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 0036689698     PISSN: 0386300X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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