Investigation of the production of intracellular cytokines by T lymphocytes using flow cytometry - Methodological problems

Epidemiologie, Mikrobiologie, Imunologie

Volumn 51, Issue 3, 2002, Pages 111-118

  Mareckova H ^a   Ravdan, A ^a   Fucikova T ^a   Janatkova I ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

Author keywords

Intracellular cytokines; Mitogens; Protein transport inhibitors

Indexed keywords

ANTICOAGULANT AGENT; BREFELDIN A; CD4 ANTIGEN; CELL PROTEIN; CYTOKINE; HEPARIN; MITOGENIC AGENT; MONENSIN; PHORBOL 13 ACETATE 12 MYRISTATE;

ACCURACY; ARTICLE; BLOOD ANALYSIS; BLOOD CULTURE; BLOOD STORAGE; CLINICAL PROTOCOL; CONTROLLED STUDY; CYTOKINE PRODUCTION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MEDICAL LITERATURE; METHODOLOGY; PROTEIN ANALYSIS; STANDARDIZATION; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION; T LYMPHOCYTE SUBPOPULATION; TIME; CHEMISTRY;

CYTOKINES; FLOW CYTOMETRY; HUMANS; T-LYMPHOCYTES;

EID: 0036674920     PISSN: 12107913     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Inability of activated cord blood T lymphocytes to perform Th1-like and Th-2 like response: Implication for transplantation
2. The value of measuring intracellular cytokines by flow cytometry
3. A new membrane permeabilization method for the detection of intracellular antigens by flow cytometry
4. Detection of intracellular cytokines by flow cytometry
5. Flow cytometric method for analysis of cytokine expression in clinical samples
6. Identification of functional subsets by flow cytometry: Intracellular detection of cytokine expression
7. Expression and kinetics of cytokines by intracellular staining using flow cytometry
8. Intracellular cytokine production by human CD4+ and CD8+ T cells from normal and immunodeficient donors using directly conjugated anti-cytokine antibodies and three-colour flow cytometry
9. Brefeldin A, but not monensin completely blocks CD69 expression on mouse lymphocytes: Efficiency of inhibitors of protein secretion in protocols for intracellular cytokine staining by flow cytometry
10. Control of lymphocytes recirculation in man: II. Differential regulation of the cutaneous lymphocytes-associated antigen, a tissue-selective homing receptor for skin - Homing cell
11. Detectionn of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies
12. Cytokine Flow cytometry: Understanding cytokine biology at single-cell level
13. Kinetics of intracytoplasmic Th1 and Th2 cytokine production assessed by flow cytometry following in vitro activation of peripheral blood mononuclear cells
14. Assessment of cytokines by immunoflorescence and the paraformaldehyde saponin procedure
15. In vivo modulation of cytokines synthesis by intravenous immunoglobulin
16. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry
17. Quantification of immunosupression by flow cytometric measurement of intracellular cytokine synthesis
18. Determination of antigenic-specific memory/effector CD4+ T cell frequencies by flow cytometry: Evidence for a novel, antigen specific homeostatic mechanism in HIV-associated immunodeficiency