A new membrane permeabilization method for the detection of intracellular antigens by flow cytometry

Journal of Immunological Methods

Volumn 124, Issue 1, 1989, Pages 103-109

  Hallden G ^a   Andersson, U ^b   Hed, J ^a   Johansson, S G O ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b ST GÖRAN S HOSPITAL   (Sweden)

Author keywords

Flow cytometry; Intracellular immunofluorescence; leukocyte; Membrane permeabilization; n octyl D glucopyranoside; Paraformaldehyde

Indexed keywords

LEUKOCYTE ANTIGEN; MONOCLONAL ANTIBODY; PARAFORMALDEHYDE; VIMENTIN;

CYTOCHEMISTRY; FLOW CYTOMETRY; HUMAN; IMMUNOFLUORESCENCE; MEMBRANE PERMEABILITY; METHODOLOGY; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; ANTIGENS, SURFACE; CELL MEMBRANE PERMEABILITY; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT ANTIBODY TECHNIQUE; FORMALDEHYDE; GLUCOSIDES; HUMAN; INTRACELLULAR MEMBRANES; LEUKOCYTES; LIGHT; POLYMERS; PRESERVATION, BIOLOGICAL; SCATTERING, RADIATION; STAINING AND LABELING; SUPPORT, NON-U.S. GOV'T; VIMENTIN;

EID: 0024435624     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(89)90191-9     Document Type: Article

References (7)

1. Enumeration of IFN-γ-producing cells by flow cytometry: comparison with fluorescence microscopy
2. A rapid method for detection of cellular proliferation using carboxyfluorescein. Assay of growth factors (IL-2, IL-1) and growth inhibiting antibodies
3. Detection of immune deposits in glomeruli: The masking effect on antigenicity of formalin in the presence of proteins
4. The use of fluorescence quenching in flow cytofluorometry to measure the attachment and ingestion phases in phagocytosis in peripheral blood without prior cell separation
5. Detection of intracytoplasmic antigens by flow cytometry
6. Detergent effects on enzyme activity and solubilization of lipid bilayer membranes