Development of a plasmepsin II fluorescence polarization assay suitable for high throughput antimalarial drug discovery

Journal of Biomolecular Screening

Volumn 7, Issue 4, 2002, Pages 367-371

  Flotow, Horst ^a   Leong, Chung Yan ^a   Buss, Antony D ^a  

^a MerLion Pharmaceuticals Pte Ltd   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIMALARIAL AGENT; AVIDIN; ESCULIN; HARMALINE; PEPSTATIN; PLASMEPSIN II; SANGUINARINE; SCOPOLETIN; STREPTAVIDIN; ASPARTIC PROTEINASE;

ACCURACY; ARTICLE; DRUG IDENTIFICATION; DRUG ISOLATION; DRUG SCREENING; ENERGY TRANSFER; ENZYME INHIBITION; FLUORESCENCE POLARIZATION; IC 50; INTERMETHOD COMPARISON; PRIORITY JOURNAL; PROTEIN EXPRESSION; RELIABILITY; ANIMAL; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; MALARIA; METABOLISM; METHODOLOGY; PARASITOLOGY; PLASMODIUM FALCIPARUM; SPECTROFLUOROMETRY;

ANIMALS; ANTIMALARIALS; ASPARTIC ENDOPEPTIDASES; DRUG EVALUATION, PRECLINICAL; ENERGY TRANSFER; MALARIA; PLASMODIUM FALCIPARUM; SPECTROMETRY, FLUORESCENCE;

EID: 0036671411     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1089/108705702320351222     Document Type: Article

References (15)

1. Plasmepsins as antimalarial targets
2. An overview of chemotherapeutic targets for antimalarial drug discovery
3. Hemoglobin metabolism in the malaria parasite Plasmodium falciparum
4. Aspartic proteases of Plasmodium falciparum and other parasitic protozoa as drug targets
5. Antimalarial synergy of cysteine and aspartic acid protease inhibitors
6. Studies on plasmepsin I and II from the malarial parasite Plasmodium falciparum and their exploitation as drug targets
7. Expression and characterization of plasmepsin I from Plasmodium falciparum
8. Structure and inhibition of plasmepsin II, a hemoglobin-degrading enzyme from Plasmodium falciparum
9. Kinetic analysis of plasmepsins I and II, aspartic proteases of the Plasmodium falciparum digestive vacuole
10. The aspartic protease from the rodent parasite Plasmodium berghei as a potential model for plasmepsins from the human malaria parasite Plasmodium falciparum
11. Fluorescence polarization and anisotropy in high throughput screening: Perspectives and primer
12. A simple statistical parameter for use in evaluation and validation of high throughput screening assays
13. High level expression and characterization of plasmepsin II II, an aspartic proteinase from Plasmodium falciparum
14. Naturally-occurring and recombinant forms of the aspartic proteinases plasmepsins I and II from the human malaria parasite Plasmodium falciparum
15. Potent low-molecular-weight non-peptide inhibitors of malarial aspartyl protease plasmepsin II