X-ray crystal structure and functional analysis of vaccinia virus K3L reveals molecular determinants for PKR subversion and substrate recognition

Molecular Cell

Volumn 10, Issue 2, 2002, Pages 295-305

  Dar, Arvin C ^a,b   Sicheri, Frank ^a  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; PROTEIN K3L; PROTEIN KINASE; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME SUBSTRATE; MOLECULAR RECOGNITION; MUTATIONAL ANALYSIS; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN STRUCTURE; REGULATORY MECHANISM; VACCINIA VIRUS; X RAY CRYSTALLOGRAPHY;

MAMMALIA; VACCINIA VIRUS;

EID: 0036670396     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(02)00590-7     Document Type: Article

References (34)

1. Characterization of pp60c-src tyrosine kinase activities using a continuous assay: Autoactivation of the enzyme is an intermolecular autophosphorylation process
2. Vaccinia virus-encoded eIF-2 alpha homolog abrogates the antiviral effect of interferon
3. Distinct patterns of IFN sensitivity observed in cells infected with vaccinia K3L- and E3L- mutant viruses
4. Crystallography and NMR system: A new software suite for macromolecular structure determination
5. The solution structure of the S1 RNA binding domain: A member of an ancient nucleic acid-binding fold
6. Recombinant vaccinia virus K3L gene product prevents activation of double-stranded RNA-dependent, initiation factor 2 alpha-specific protein kinase
7. Double-stranded-RNA-dependent protein kinase and TAR RNA-binding protein form homo- and heterodimers in vivo
8. The kinase insert domain of interferon-induced protein kinase PKR is required for activity but not for interaction with the pseudosubstrate K3L
9. The vaccinia virus K3L gene product potentiates translation by inhibiting double-stranded-RNA-activated protein kinase and phosphorylation of the alpha subunit of eukaryotic initiation factor 2
10. The E3L and K3L vaccinia virus gene products stimulate translation through inhibition of the double-stranded RNA-dependent protein kinase by different mechanisms
11. Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods
12. Gene-specific regulation by general translation factors
13. Interaction of the interferon-induced PKR protein kinase with inhibitory proteins P58IPK and vaccinia virus K3L is mediated by unique domains: Implications for kinase regulation
14. Translational initiation factors IF-1 and eIF-2 alpha share an RNA-binding motif with prokaryotic ribosomal protein S1 and polynucleotide phosphorylase
15. The (2′-5′) oligoadenylate (pppA2′-5′A2′-5′A) synthetase and protein kinase(s) from interferon-treated cells
16. Crystal structure of the cytoplasmic domain of the type I TGF beta receptor in complex with FKBP12
17. The TGF beta receptor activation process: An inhibitor- to substrate-binding switch
18. The Eleventh Datta Lecture. The structural basis for substrate recognition and control by protein kinases
19. Improved methods for binding protein models in electron density maps and the localization of errors in these models
20. Regulation of the protein kinase PKR by the vaccinia virus pseudosubstrate inhibitor K3L is dependent on residues conserved between the K3L protein and the PKR substrate eIF2alpha
21. Crystal structures of a schistosomal drug and vaccine target: Glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel
22. A synthetic peptide substrate for initiation factor-2 kinases
23. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
24. Crystal structure of the N-terminal segment of human eukaryotic translation initiation factor 2alpha
25. Processing of X-ray diffraction data collected in oscillation mode
26. Expression of vaccinia virus K3L protein in yeast inhibits eukaryotic initiation factor-2 kinase GCN2 and the general amino acid control pathway
27. Homologous regions of the alpha subunit of eukaryotic translational initiation factor 2 (eIF2alpha) and the vaccinia virus K3L gene product interact with the same domain within the dsRNA-activated protein kinase (PKR)
28. Pancreatic eukaryotic initiation factor-2alpha kinase (PEK) homologues in humans, Drosophila melanogaster and Caenorhabditis elegans that mediate translational control in response to endoplasmic reticulum stress
29. How cells respond to interferons
30. Heterologous dimerization domains functionally substitute for the double-stranded RNA binding domains of the kinase PKR
31. Mechanism of activation of the double-stranded-RNA-dependent protein kinase, PKR: Role of dimerization and cellular localization in the stimulation of PKR phosphorylation of eukaryotic initiation factor-2 (eIF2)
32. Inhibitory sequences in the N-terminus of the double-stranded-RNA- dependent protein kinase, PKR, are important for regulating phosphorylation of eukaryotic initiation factor 2alpha (eIF2alpha)
33. Signal integration via PKR
34. Structural basis for autoinhibition of the Ephb2 receptor tyrosine kinase by the unphosphorylated juxtamembrane region