SH3-SH2 Domain Orientation in Src Kinases: NMR Studies of Fyn

Structure

Volumn 10, Issue 7, 2002, Pages 901-911

  Ulmer, Tobias S ^a   Werner, Jörn M ^a   Campbell, Iain D ^a,b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Domain orientation; Dynamic domain coupling; Residual dipolar coupling; Rotational diffusion anisotropy; SH3 SH2; Src kinase

Indexed keywords

PROTEIN KINASE FYN; PROTEIN TYROSINE KINASE; UNCLASSIFIED DRUG; FYN PROTEIN, HUMAN; LIGAND; ONCOPROTEIN; PEPTIDE;

ANISOTROPY; ARTICLE; DIFFUSION; DISSOCIATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME ISOLATION; ENZYME KINETICS; PRIORITY JOURNAL; PROTEIN BINDING; PROTON NUCLEAR MAGNETIC RESONANCE; SRC HOMOLOGY DOMAIN; CHEMICAL STRUCTURE; CHEMISTRY; HUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

HUMAN; LIGANDS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; PEPTIDES; PROTO-ONCOGENE PROTEINS; SRC HOMOLOGY DOMAINS; SRC-FAMILY KINASES; SUPPORT, NON-U.S. GOV'T; HUMANS; PROTO-ONCOGENE PROTEINS C-FYN;

EID: 0036652846     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(02)00781-5     Document Type: Article

References (52)

1. Three-dimensional structure of the tyrosine kinase c-Src
2. The 2.35 angstrom crystal structure of the inactivated form of chicken Src: A dynamic molecule with multiple regulatory interactions
3. Crystal structure of the Src family tyrosine kinase Hck
4. Structures of Src-family tyrosine kinases
5. Cellular functions regulated by Src family kinases
6. Csk Inhibition of c-Src activity requires both the Sh2 and Sh3 domains of Src
7. Activation of the Src-family tyrosine kinase Hck by SH3 domain displacement
8. Dynamic coupling between the SH2 and SH3 domains of c-Src and hck underlies their inactivation by C-terminal tyrosine phosphorylation
9. SH2- and SH3-mediated interactions between focal adhesion kinase and Src
10. The role of the Src homology 3-Src homology 2 interface in the regulation of Src kinases
11. Identification and characterization of the hamster polyomavirus middle T-antigen
12. Probing the "two-pronged plug two-holed socket" model for the mechanism of binding of the Src SH2 domain to phosphotyrosyl peptides: A thermodynamic study
13. Binding of a high-affinity phosphotyrosyl peptide to the Src Sh2 domain - Crystal-structures of the complexed and peptide-free forms
14. Alternative modes of tyrosyl phosphopeptide binding to a Src family SH2 domain: Implications for regulation of tyrosine kinase activity
15. Solution structure and peptide binding of the SH3 domain from human Fyn
16. The SH2 domain from the tyrosine kinase Fyn in complex with a phosphotyrosyl peptide reveals insights into domain stability and binding specificity
17. Heteronuclear NMR studies of the combined Src homology domains 2 and 3 of pp60 c-Src: Effects of phosphopeptide binding
18. 2 binding orientations for peptides to the Src Sh3 domain - Development of a general-model for Sh3-ligand interactions
19. Nuclear spin relaxation in ellipsoids undergoing rotational Brownian motion
20. Long-range motional restrictions in a multidomain zinc-finger protein from anisotropic tumbling
21. Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium
22. Crystal-structure of the Sh3 domain in human Fyn - Comparison of the 3-dimensional structures of Sh3 domains in tyrosine kinases and spectrin
23. Structural basis for the binding of proline-rich peptides to Sh3 domains
24. High-resolution nuclear magnetic resonance spectra of orientated molecules
25. Direct determination of changes of interdomain orientation on ligation: Use of the orientational dependence of N-15 NMR relaxation in Abl SH(32)
26. Hydro - A computer-program for the prediction of hydrodynamic properties of macromolecules
27. Hydrodynamic properties of complex, rigid, biological macromolecules - Theory and applications
28. Comparison of SH3 and SH2 domain dynamics when expressed alone or in an SH(3+2) construct: The role of protein dynamics in functional regulation
29. The solution structure of Abl Sh3, and its relationship to Sh2 in the Sh(32) construct
30. Orienting domains in proteins using dipolar couplings measured by liquid-state NMR: Differences in solution and crystal forms of maltodextrin binding protein loaded with beta-cyclodextrin
31. Use of dipolar H-1-N-15 and H-1-C-13 couplings in the structure determination of magnetically oriented macromolecules in solution
32. Order matrix analysis of residual dipolar couplings using singular value decomposition
33. Defining long range order in NMR structure determination from the dependence of heteronuclear relaxation times on rotational diffusion anisotropy
34. Structure of the regulatory domains of the Src-family tyrosine kinase Lck
35. Nonreceptor tyrosine protein-kinases
36. Regulation, substrates and functions of src
37. Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values
38. Backbone dynamics of a free and a phosphopeptide-complexed Src homology 2 domain studied by N-15 NMR relaxation
39. The generation of phase-sensitive 2d N-15-H-1 spectra using gradient pulses for coherence-transfer-pathway selection
40. The importance of not saturating H2O in protein NMR - Application to sensitivity enhancement and NOE measurements
41. TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins
42. A heteronuclear correlation experiment for simultaneous determination of N-15 longitudinal decay and chemical-exchange rates of systems in slow equilibrium
43. Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra
44. Model-free approach to the interpretation of nuclear magnetic-resonance relaxation in macromolecules. 1. Theory and range of validity
45. The use of residual dipolar coupling in concert with backbone relaxation rates to identify conformational exchange by NMR
46. Efficient analysis of macromolecular rotational diffusion from heteronuclear relaxation data
47. Shape and dynamics of a calcium binding EGF domain pair investigated by 15N-NMR relaxation
48. A novel interactive tool for rigid-body modeling of multi-domain macromolecules using residual dipolar couplings
49. Prediction of sterically induced alignment in a dilute liquid crystalline phase: Aid to protein structure determination by NMR
50. Intramolecular interactions of the regulatory domains of the Bcr-Abl kinase reveal a novel control mechanism