A comparison of hydraulic and laser capture microdissection methods for collection of single B cells, PCR, and sequencing of antibody VDJ

Analytical Biochemistry

Volumn 306, Issue 1, 2002, Pages 55-62

  Obiakor, Harold ^a   Sehgal, Devinder ^a,b   Dasso, Joseph F ^a   Bonner, Robert F ^a   Malekafzali, Arash ^a   Mage, Rose G ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

B cells; Immunoglobulin VDJ; Microdissection; Single cell PCR

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; PRIMER DNA; WATER; ANTIBODY; GENE PRODUCT;

ANIMAL; ANTIBODY SPECIFICITY; APPENDIX; ARTICLE; B LYMPHOCYTE; CELL SEPARATION; COMPARATIVE STUDY; CYTOLOGY; DISSECTION; DNA SEQUENCE; GENETICS; HUMAN; INSTRUMENTATION; LASER; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RABBIT; SPLEEN; ALLELE; ANIMAL TISSUE; GENE REARRANGEMENT; GENE SEQUENCE; GERMINAL CENTER; HUMAN TISSUE; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL;

ANIMALS; ANTIBODY DIVERSITY; APPENDIX; B-LYMPHOCYTES; BASE SEQUENCE; CELL SEPARATION; DISSECTION; DNA PRIMERS; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; LASERS; MICE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RABBITS; SEQUENCE ANALYSIS, DNA; SPLEEN; WATER;

ANTIBODIES; CLONE CELLS; ENCODING (SYMBOLS); GENES; LYMPHOCYTES; MICRODISSECTION; MICROMANIPULATORS; POLYMERASE CHAIN REACTION; TISSUE;

B CELLS; GENE REARRANGEMENT; HEAVY AND LIGHT CHAINS; IMMUNOGLOBULIN VDJ; LASER CAPTURE MICRODISSECTION; MICRO MANIPULATION; PCR AMPLIFICATION; SINGLE CELLS;

CYTOLOGY;

EID: 0036629197     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5671     Document Type: Article

References (13)

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