The degradation of myofibrillar proteins in beef and lamb using denaturing electrophoresis - An overview

Journal of Muscle Foods

Volumn 13, Issue 2, 2002, Pages 81-102

  Hopkins, D L ^a   Thompson, J M ^b  

^a NSW AGRICULTURE   (Australia)

^b UNIVERSITY OF NEW ENGLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DEGRADATION; DELAMINATION; DENSITOMETERS; ELECTROPHORESIS; IMAGE ANALYSIS; MEATS;

MYOFIBRILLAR PROTEINS;

PROTEINS;

LAMB;

EID: 0036613820     PISSN: 10460756     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-4573.2002.tb00323.x     Document Type: Review

References (71)

1. Postmortem degradation of titin and nebulin of beefsteaks varying in tenderness
2. An immunological method to assess protein degradation in post-mortem muscle
3. Effects of postmortem storage and temperature on muscle protein degradation: Analysis by SDS gel electrophoresis
4. Effect of postmortem storage on the Z-line region of titin in bovine muscle
5. Standardisation and application of semi-quantitative SDS-PAGE method for measurement of myofibrillar protein fragments in bovine longissimus muscle
6. Quantification of beef myofibrillar proteins by SDS-PAGE
7. Beef myofibrillar protein salt solubility in relation to tenderness and proteolysis
8. Studies in meat tenderness II. Proteolysis and the aging of beef
9. Studies in meat tenderness: 6. The nature of myofibrillar proteins extracted from meat during aging
10. When the glue comes unstuck
11. Modelling postmortem tenderization-1. Texture of electrically stimulated and non-stimulated beef
12. Free amino acids in bovine muscles and their relationship to tenderness
13. Changes in titin and nebulin in postmortem bovine muscle revealed by gel electrophoresis, western blotting and immunfluorescence microscopy
14. Titin content of beef in relation to tenderness
15. Repetitive titin epitopes with a 42 nm spacing coincide in relative position with known A band striations also identified by major myosin-associated proteins. An immunoelectron-microscopial study on myofibrils
16. The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: A map often nonrepetitive epitopes starting at the Z line extends close to the M line
17. Effect of calpastatin on degradation of myofibrillar proteins by mu-calpain under postmortem conditions
18. Determinants of tenderization in beef longissimus dorsi and triceps brachii muscles
19. Role of proteinases and protein turnover in muscle growth and meat quality
20. Studies of the á-actinin/actin interaction in the Z-disk by using calpain
21. The calpain system and skeletal muscle growth
22. Titin and tenderness
23. Post-mortem changes in myofibrillar proteins in relation to meat texture
24. Trends in skeletal muscle biology and the understanding of toughness in beef
25. Identification of the 30 kDa polypeptide in post mortem skeletal muscle as a degradation product of troponin-T
26. Effect of electrical stimulation on postmortem titin, nebulin, desmin, and troponin-T degradation and ultrastructural changes in bovine longissimus muscle
27. Effects of electrical stimulation and postmortem storage on changes in titin, nebulin, desmin, troponin-T, and muscle ultrastructure in Bos indicus crossbred cattle
28. Inhibition of protease activity: Part 2. Degradation of myofibrillar proteins, myofibril examination and determination of free calcium levels
29. Factors contributing to proteolysis and disruption of myofibrillar proteins and the impact on tenderization in beef and lamb meat
30. Proteolysis of specific muscle structural proteins by mu-calpain at low pH and temperature is similar to degradation in postmortem bovine muscle
31. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting comparisons of purified myofibrils and whole muscle preparations for evaluating titin and nebulin in postmortem muscle
32. Effects of postmortem aging time, animal age, and sex on degradation of titin and nebulin in bovine longissimus muscle
33. Biochemical factors regulating the toughening and tenderization processes of meat
34. Effect of postmortem storage on cold-shortened bovine muscle: Analysis by SDS-polyacrylamide gel electrophoresis
35. Effects of postmortem storage on muscle protein degradation: Analysis by SDS-polyacrylamide gel electrophoresis
36. Titins: Giant proteins in charge of muscle ultrastructure and elasticity
37. The giant protein titin. Emerging roles in physiology and pathophysiology
38. Effect of postmortem storage on degradation of the myofibrillar protein titin in bovine longissimus muscle
39. Connectin/titin, giant elastic protein of muscle
40. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250
41. Relationship of myofibril fragmentation index to measures of beefsteak tenderness
42. Myofibril fragmentation and shear resistance of three bovine muscles during postmortem storage
43. Proteolytic and physicochemical mechanisms involved in meat texture development
44. SDS-PAGE conditions for detection of titin and nebulin in tender and tough bovine muscles
45. Relationship between toughness and troponin T in conditioned beef
46. Measurement of autolysis in beef muscle homogenates
47. Combination of low voltage electrical stimulation and early postmortem temperature conditioning on degradation of myofibrillar proteins in Korean native cattle (Hanwoo)
48. Change in titin position in postmortem bovine muscle
49. Postmortem changes in the myofibrillar and other cytoskeletal proteins in muscle
50. Biochemical and structural properties of titin, nebulin and intermediate filaments in muscle
51. Structure and proteins of the exosarcomeric muscle cell cytoskeleton
52. Calmodulin-sensitive interaction of human nebulin fragments with actin and myosin
53. Bovine muscle shortening and protein degradation after electrical stimulation, excision and chilling
54. SYPRO orange and SYPRO red protein gel stares: One-step fluorescent staining of denaturing gels for detection of nanogram levels of protein
55. Calcium-induced splitting of connectin filaments into β-connection and a 1,200-kDa subfragment
56. Post-mortem changes in skeletal muscle connectin
57. Purification and characterization of the 1,200-kDa subfragment of connectin filaments produced by 0.1 mM calcium ions
58. Is Z-disk degradation responsible for postmortem tenderisation?
59. Electrophoretic analysis of changes in beef myofibrillar proteins during the early post-mortem period
60. The effect of electric stimulation on beef tenderness, protease activity and myofibrillar protein fragmentation
61. Effects of exogenous protease effectors on beef tenderness development and myofibrillar degradation and solubility
62. Effect of meat ultimate pH on rate of titin and nebulin degradation
63. The effect of postmortem time of injection and freezing on the effectiveness of calcium chloride for improving beef tenderness
64. Prerigor and postrigor changes in tenderness of ovine longissimus muscle
65. The extent of proteolysis is independent of sarcomere length in lamb longissimus and psoas major
66. Ionic strength and myofibrillar protein solubilization
67. Effect of temperature and pH on the post-mortem degradation of myofibrillar proteins
68. Postmortem changes in cytoskeletal proteins of muscle
69. Molecular structure of the sarcomeric Z-disk: Two types of titin interactions lead to an asymmetrical sorting of α-actinin
70. Effects of postmortem pH and temperature on bovine muscle structure and meat tenderness