Three-step purification of gp96 from human liver tumor tissues suitable for isolation of gp96-bound peptides

Journal of Immunological Methods

Volumn 264, Issue 1-2, 2002, Pages 29-35

  Meng, Song Dong ^a   Song, Jian ^a   Rao, Zihe ^b   Tien, Po ^a   Gao, George F ^d  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b TSINGHUA UNIVERSITY   (China)

^c HARVARD MEDICAL SCHOOL   (United States)

^d HARVARD UNIVERSITY   (United States)

Author keywords

gp96; Peptide isolation; Purification; Tumor tissues

Indexed keywords

AMMONIUM SULFATE; CONCANAVALIN A; GLYCOPROTEIN GP 96; HEAT SHOCK PROTEIN; SEPHAROSE;

ADULT; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; FEMALE; HUMAN; HUMAN TISSUE; LIVER CELL CARCINOMA; MALE; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN PURIFICATION; REPRODUCIBILITY; TECHNIQUE;

AMMONIUM SULFATE; ANTIGENS, NEOPLASM; BASE SEQUENCE; CARCINOMA, HEPATOCELLULAR; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; HEPATITIS B CORE ANTIGENS; HEPATITIS B VIRUS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; LIVER NEOPLASMS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; PEPTIDES; PRECIPITATION; PROTEIN BINDING; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN;

EID: 0036606130     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00093-5     Document Type: Article

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